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Development of Protein Capped Nano Gold for NIR Photothermal and Molecular Imaging Applications for Diagnosis of Cancer Cells: In Vitro Studies

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Abstract

The current work demonstrated an in vitro model of diastase capped gold nanoparticles (Au NPs) and nanoplates (Au NPts) as unique contrast agents for molecular imaging and photothermal cancer therapy, respectively. The Au NPs/Au NPts were fabricated and conjugated with monoclonal antibodies of anti-epidermal growth factor receptor (anti-EGFR). Anti-EGFR-AuNPs bioconjugate was studied for molecular imaging allowing to interact with the nasopharyngeal carcinoma (CNE2 cells). Confocal immunofluorescence microscopic results revealed the increased scattering and reflectance properties of CNE2 cells after attachment and localization of Anti-EGFR-AuNPs bioconjugate on the cell surface, consequently providing the good optical contrast for the cancer cell imaging. On the other hand, Anti-EGFR-Au NPts were studied for photothermal applications using two malignant oral epithelial cell lines (HSC 3 and HOC 313 clone 8) and a nonmalignant epithelial cell line (HaCat), which revealed that the malignant cells require almost half of the laser energy to be photothermally eliminated after exposure to continuous red laser at 800 nm when compared with the nonmalignant cells. Further, the present approach may have potential for development of NPs for selective photothermal therapy and a successful diagnostics of cancer cell for bioimaging applications.

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References

  1. I. H. El-Sayed, X. Huang, and M. A. El-Sayed (2005). Selective laser photo-thermal therapy of epithelial carcinoma using anti-EGFR antibody conjugated gold nanoparticles. Cancer Lett. 239, 129–135.

    Article  Google Scholar 

  2. C. Loo, A. Lowery, N. Halas, J. West, and R. Drezek (2005). Immunotargeted nanoshells for integrated cancer imaging and therapy. Nano Lett. 5, 709–711.

    Article  CAS  Google Scholar 

  3. R. Weissleder (2001). A clearer vision for in vivo imaging. Nat. Biotechnol. 19, 316–317.

    Article  CAS  Google Scholar 

  4. N. W. Shi Kam, M. O’Connell, J. A. Wisdom, and H. Dai (2005). Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destruction. Proc. Natl. Acad. Sci. USA 102, 11600–11605.

    Article  Google Scholar 

  5. E. Hao and G. C. Schatz (2004). Electromagnetic fields around silver nanoparticles and dimers. J. Chem. Phys. 120, 357–366.

    Article  CAS  Google Scholar 

  6. E. Hao, G. C. Schatz, and J. T. Hupp (2004). Synthesis and optical properties of anisotropic metal nanoparticles. J. Fluoresc. 14, 331–341.

    Article  CAS  Google Scholar 

  7. F. Koenig, J. Knittel, and H. Stepp (2001). Diagnosing cancer in vivo. Science 292, 1401–1403.

    Article  CAS  Google Scholar 

  8. K. Sokolov, J. Aaron, et al. (2003). Optical systems for in vivo molecular imaging of cancer. Technol. Cancer Res. Treat. 2, 491–504.

    Article  CAS  Google Scholar 

  9. K. Sokolov, M. Follen, J. Aaron, I. Pavlova, A. Malpica, R. Lotan, and R. Richards-Kortum (2003). Real-time vital optical imaging of precancer using anti-epidermal growth factor receptor antibodies conjugated to gold nanoparticles. Cancer Res. 63, 1999–2004.

    CAS  PubMed  Google Scholar 

  10. P. Mulvaney (1996). Surface plasmon spectroscopy of nanosized metal particles. Langmuir 12, 788–800.

    Article  CAS  Google Scholar 

  11. W. D. Geoghegan and G. A. Ackerman (1977). Adsorption of horseradish peroxidase, ovomucoid, and antiimmunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin, and goat anti-human immunoglobulin G on cell surfaces at the electron microscope level: a new method, theory, and application. J. Histochem. Cytochem. 25, 1187–1200.

    Article  CAS  Google Scholar 

  12. D. M. Shin, J. Y. Ro, W. K. Hong, and W. N. Hittelman (1994). Dysregulation of epidermal growth factor receptor expression in premalignant lesions during head and neck tumorigenesis. Cancer Res. 54, 3153–3159.

    CAS  PubMed  Google Scholar 

  13. I. H. El-Sayed, X. Huang, and M. A. El-Sayed (2005). Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated gold nanoparticles in cancer diagnostics: applications in oral cancer. Nano Lett. 5, 829–834.

    Article  CAS  Google Scholar 

  14. J. C. Kah, C. J. Sheppard, C. G. Lee, M. C. Olivo, Application of antibody-conjugated gold nanoparticles for optical molecular imaging of epithelial carcinoma cells. in Nanobiophotonics and Biomedical Applications III (International Society for Optics and Photonics, 2006), p. 609503.

  15. M. J. Abrams and B. A. Murrer (1993). Metal compounds in therapy and diagnosis. Science 261, 725–730.

    Article  CAS  Google Scholar 

  16. M. Sireesh Babu, M. Badal Kumar, R. Shivendu, and D. Nandita (2015). Diastase assisted green synthesis of size controllable gold nanoparticles. RSC Adv. 5, 26727.

    Article  Google Scholar 

  17. A. M. E. Nouri, C. Thompson, H. Cannell, M. Symes, S. Purkiss, and Z. Amirghofran (2000). Profile of epidermal growth factor receptor (EGFr) expression in human malignancies: effects of exposure to EGF and its biological influence on established human tumor cell lines. Int. J. Mol. Med. 6, 495–500.

    CAS  PubMed  Google Scholar 

  18. S. B. Maddinedi, B. K. Mandal, and K. K. Anna (2017). Environment friendly approach for size controllable synthesis of biocompatible Silver nanoparticles using diastase. Environ. Toxicol. Pharmacol. 49, 131–136.

    Article  CAS  Google Scholar 

  19. A. J. Shnoudeh, I. Hamad, R. W. Abdo, L. Qadumii, A. Y. Jaber, H. S. Surchi, and S. Z. Alkelany, Synthesis, characterization, and applications of metal nanoparticles. In Biomaterials and Bionanotechnology. (Academic Press, Boca Raton, 2019), pp. 527–612.

    Google Scholar 

  20. R. S. Herbst and D. M. Shin (2002). Monoclonal antibodies to target epidermal growth factor receptor–positive tumors. Cancer 94, 1593–1611.

    Article  CAS  Google Scholar 

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Acknowledgements

Authors are thankful to Wisdom Health care Institute, Chongqing City Management College, Chongqing for their support for this research work.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Smart Healthcare Industry, Chongqing City Management College, No. 151 South 2nd Road, Huxi University Town, Gaoxin District, Chongqing, 401331, China

    Ke Tao

  2. School of Foreign Languages Studies, Zhejiang Sci-Tech University, 5 Second Avenue, Xiasha Higher Education Zone, Hangzhou, 310018, China

    Gopi Krishna Murakonda

  3. Department of Physical Education and Sports, Guntur Medical College, Guntur, Andhra Pradesh, 522004, India

    Ramu Jarubula

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  1. Ke Tao
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  2. Gopi Krishna Murakonda
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  3. Ramu Jarubula
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Correspondence to Ramu Jarubula.

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Tao, K., Murakonda, G.K. & Jarubula, R. Development of Protein Capped Nano Gold for NIR Photothermal and Molecular Imaging Applications for Diagnosis of Cancer Cells: In Vitro Studies. J Clust Sci 33, 2643–2650 (2022). https://doi.org/10.1007/s10876-021-02179-1

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  • DOI: https://doi.org/10.1007/s10876-021-02179-1

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