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GFT projection NMR for efficient 1H/13C sugar spin system identification in nucleic acids

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Abstract

A newly implemented G-matrix Fourier transform (GFT) (4,3)D HC(C)CH experiment is presented in conjunction with (4,3)D HCCH to efficiently identify 1H/13C sugar spin systems in 13C labeled nucleic acids. This experiment enables rapid collection of highly resolved relay 4D HC(C)CH spectral information, that is, shift correlations of 13C–1H groups separated by two carbon bonds. For RNA, (4,3)D HC(C)CH takes advantage of the comparably favorable 1′- and 3′-CH signal dispersion for complete spin system identification including 5′-CH. The (4,3)D HC(C)CH/HCCH based strategy is exemplified for the 30-nucleotide 3′-untranslated region of the pre-mRNA of human U1A protein.

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Abbreviations

GFT:

G-matrix Fourier transformation

r.f.:

Radio-frequency

RNA:

Ribonucleic acid

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Acknowledgments

This work was supported by the National Science Foundation (MCB 0817857 to T.S., and MCB 051644 to G.V.), the Department of Science and Technology, India (to H.S.A.), and by the CSIR, India (fellowship to G.J.) We thank Dr. A. Eletski for helpful discussions.

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Authors and Affiliations

  1. Department of Chemistry, State University of New York at Buffalo, Buffalo, NY, 14260, USA

    Bharathwaj Sathyamoorthy, Victor Beaumont & Thomas Szyperski

  2. NMR Research Centre, Indian Institute of Science, Bangalore, 560012, India

    Hanudatta S. Atreya & Garima Jaipuria

  3. Department of Chemistry, University of Washington, Seattle, WA, 98195, USA

    Gabriele Varani

  4. Department of Biochemistry, University of Washington, Seattle, WA, 98195, USA

    Gabriele Varani

Authors
  1. Hanudatta S. Atreya
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  2. Bharathwaj Sathyamoorthy
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  3. Garima Jaipuria
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  4. Victor Beaumont
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  5. Gabriele Varani
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  6. Thomas Szyperski
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Corresponding author

Correspondence to Thomas Szyperski.

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Atreya, H.S., Sathyamoorthy, B., Jaipuria, G. et al. GFT projection NMR for efficient 1H/13C sugar spin system identification in nucleic acids. J Biomol NMR 54, 337–342 (2012). https://doi.org/10.1007/s10858-012-9687-5

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