Summary
Backbone-driven assignment methods that utilize covalent connectivities have greatly facilitated spectral assignments of proteins. In nucleic acids, 1H−13C−31P correlations could play a similar role, and several related experiments (HCP) have recently been presented for backbone-driven sequential assignments in RNA. The three-dimensional extension of 1H−31P Het-Cor (P,H-COSY-H,C-HMQC) and Het-TOCSY (P,H-TOCSY-H,C-HMQC) experiments presented here complements HCP experiments as tools for spectral assignments and extraction of dihydral angle constraints. By relying on 1H−31P rather than 13C−31P couplings to generate cross peaks, the strongest connectivities are observed in different spectral regions, increasing the likelihood of resolving spectral overlap. In addition, semiquantitative estimates of 1H−31P and 13C−31P couplings provide dihedral angle constraints for RNA structure determination.
This is a preview of subscription content, log in via an institution to check access.
References
Altona, C. (1982) Recl. Trav. Chim. Pays-Bas, 101, 413–433.
Gehring, K., Leroy, J.-L. and Gueron, M. (1993) Nature, 363, 561–565.
Heus, H.A., Wijmenga, S.S., Van deVen, F.J.M. and Hilbers, C.W. (1994) J. Am Chem. Soc., 116, 4983–4984.
Hines, J.V., Landry, S.M., Varani, G. and TinocoJr., I. (1994) J. Am. Chem. Soc., 116, 5823–5831.
Hines, J.V., Varani, G., Landry, S.M. and TinocoJr., I. (1993) J. Am. Chem. Soc., 115, 11002–11003.
Kellogg, G.W. (1992) J. Magn. Reson., 98, 176–182.
Kellogg, G.W. and Schweitzer, B.I. (1993) J. Biomol. NMR, 3, 577–595.
Kellogg, G.W., Szewczak, A.A. and Moore, P.B. (1992) J. Am. Chem. Soc. 114, 2727–2728.
Marino, J.P., Schwalbe, H., Anklin, C., Bermel, W., Crothers, D.M. and Griesinger, C. (1994) J. Am. Chem. Soc., 116, 6472–6473.
Marino, J.P., Schwalbe, H., anklin, C., Bermel, W., Crothers, D.M. and Griesinger, C. (1995) J. Biomol. NMR, 5, 87–92.
Mooren, M.M.W., Wijmenga, S.S., Van derMarel, G.A., VanBoom, J.H. and Hilbers, C.W. (1994) Nucleic Acids Res., 22, 2658–2666.
Nikonowicz, E.P. and Pardi, A. (1993) J. Mol. Biol., 232, 1141–1156.
Saenger, W. (1984) Principles of Nucleic Acid Structure, Springer Verlag, New York, NY.
Schmieder, P., Ippel, J.H., Van denElst, H., Van derMarel, G.A., VanBoom, J.H., Altona, C. and Kessler, H. (1992) Nucleic Acids Res., 20, 4747–4751.
Schwalbe, H., Marino, J.P., King, G.C., Wechselberger, R., Bermel, W. and Griesinger, C., (1994) J. Biomol. NMR, 4, 631–644.
Schwalbe, H., Samstag, W., Engels, J.W., Bermel, W. and Griesinger, C. (1993) J. Biomol. NMR, 3, 479–486.
Shaka, A.J., Barker, P. and Freeman, R. (1985) J. Magn. Reson., 64, 547–552.
Sklenář, V., Miyashiro, H., Zon, G. and Bax, A. (1986) FEBS Lett., 208, 94–98.
Szewczak, A.A., Moore, P.B., Chan, Y.-L. and Wool, I.G. (1993) Proc. Natl. Acad. Sci. USA, 90, 9581–9585.
Tate, S.-I., Ono, A. and Kainosho, M. (1995) J. Magn. Reson. Ser. B, 106, 89–91.
VanGelder, C.W.G., Gunderson, S.I., Jansen, E.J.R., Boelens, W.C., Polycarpou-Schwartz, M., Mattaj, I.W. and Vanvenrooij, W.J., (1993) EMBO J., 12 5191–5200.
Varani, G., Cheong, C. and TinocoJr., I. (1991) Biochemistry 30, 3280–3289.
Varani, G. and TinocoJr., I. (1991a) J. Am. Chem. Soc., 113, 9349–9354.
Varani, G. and TinocoJr., I. (1991b) Q.Rev. Biophys., 24, 479–532.
Wijmenga, S.S., Heus, H.A., Leeuw, H.A.E., Hoppe, H., Van derGraaf, M. and Hilbers, C.W. (1995) J. Biomol. NMR, 5, 82–86.
Wimberly, B.T. (1992) Ph.D. Thesis, University of California, Berkeley, CA.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Varani, G., Aboul-ela, F., Allain, F. et al. Novel three-dimensional 1H−13C−31P triple resonance experiments for sequential backbone correlations in nucleic acids. J Biomol NMR 5, 315–320 (1995). https://doi.org/10.1007/BF00211759
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00211759