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The effect of PLGA sphere diameter on rabbit mesenchymal stem cells in adipose tissue engineering

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Abstract

To investigate the effect of injectable PLGA sphere’s diameter on adipose tissue engineering, rabbit mesenchymal stem cells were attached to various diameters of injectable PLGA spheres (<75; 75–100; 100–150; 150–200; and 200–250 μm). These five groups were cultured in adipogenic media for 2 weeks in vitro and injected into necks of nude mice. Prior to in vivo study, cell proliferation and adipogenic differentiation were determined by hexosaminidase assay and Oil red O staining after 2 weeks. Group C (100–150 μm) showed the highest adipogenic differentiation and the proliferation capacity of Group B (75–100 μm) was significantly higher than that of any other group. We harvested newly formed tissues from necks of nude mice after 1 and 4 weeks. Although PLGA spheres have not been degraded and there was no significant histological difference among various sizes of spheres after 1 week, well-organized fat pads (PLGA spheres were completely degraded) could be observed, and the histology of the 100–150 μm groups resembled that of native tissue after 4 weeks. Based on these experiments, we could conclude that the optimal size of PLGA spheres for adipogenesis was 100–150 μm.

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Acknowledgements

This research was supported by the Ministry of Commerce, Industry and Energy (project number 10005680), Korea.

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Authors and Affiliations

  1. Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752, South Korea

    Yu Suk Choi, Si-Nae Park & Hwal Suh

  2. BK21 Project Team of Nanobiomaterials for the Cell-based Implants, Yonsei University, Seongsanno 250, Seodaemun-gu, Seoul, 120-752, South Korea

    Si-Nae Park & Hwal Suh

Authors
  1. Yu Suk Choi
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  2. Si-Nae Park
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  3. Hwal Suh
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Correspondence to Hwal Suh.

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Choi, Y.S., Park, SN. & Suh, H. The effect of PLGA sphere diameter on rabbit mesenchymal stem cells in adipose tissue engineering. J Mater Sci: Mater Med 19, 2165–2171 (2008). https://doi.org/10.1007/s10856-007-3320-2

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