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Host–parasite interactions and host species susceptibility of the marine oomycete parasite, Olpidiopsis sp., from Korea that infects red algae

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Abstract

Porphyra farms in Korea occasionally suffer from Olpidiopsis infection. As Porphyra farming proceeds from October to March, this obligatory biotrophic parasite may need an alternative host to survive during other months of the year. To find a possible alternative summer host, we collected algae from Wando, Korea, where extensive Porphyra plantations are located, and discovered an oomycete assignable to the genus Olpidiopsis from Heterosiphonia pulchra. Host susceptibility tests showed that this oomycete could also infect Heterosiphonia japonica, Dasya sp., Dasysiphonia chejuensis, and also blades of Porphyra tenera. The minimum incubation time for this Olpidiopsis sp. to infect its hosts was approximately 4 h. Zoosporangia matured in 2 days and biflagellate zoospores were released. Free zoospores remained infective in seawater for up to 7 days. The infection of Olpidiopsis sp. to H. japonica was cell-type specific and extended rhizoid-like apical cells of determinate branches were preferentially infected. FITC-conjugated lectin staining showed specific binding of concanavalin A (ConA) to extended rhizoid-like apical cells. Attachment of Olpidiopsis sp. zoospores to the host cells was inhibited by α-mannosidase. Monosaccharide inhibition experiments showed that d(+)-mannose, complementary to the lectin ConA, could also block the infection, suggesting a lectin–carbohydrate interaction during host–parasite recognition.

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Acknowledgments

This research was supported by a grant (RP-2011-AQ-009) from National Fisheries Research and Development Institute, Korea. We thank Dr. John A. West (University of Melbourne) for sending us several algal isolates used in the susceptibility experiment.

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Authors and Affiliations

  1. Department of Biology, Kongju National University, Kongju, Chungnam, 314-701, South Korea

    Tatyana A. Klochkova, Jun Bo Shim & Gwang Hoon Kim

  2. National Fisheries Research and Development Institute (NFRDI), Mokpo, South Korea

    Mi Sook Hwang

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  1. Tatyana A. Klochkova
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  2. Jun Bo Shim
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  4. Gwang Hoon Kim
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Correspondence to Gwang Hoon Kim.

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Klochkova, T.A., Shim, J.B., Hwang, M.S. et al. Host–parasite interactions and host species susceptibility of the marine oomycete parasite, Olpidiopsis sp., from Korea that infects red algae. J Appl Phycol 24, 135–144 (2012). https://doi.org/10.1007/s10811-011-9661-8

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  • DOI: https://doi.org/10.1007/s10811-011-9661-8

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