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Corynoline Exhibits Anti-inflammatory Effects in Lipopolysaccharide (LPS)-Stimulated Human Umbilical Vein Endothelial Cells through Activating Nrf2

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Abstract

Corynoline, a bioactive compound isolated from Corydalis bungeana Turcz., has been known to have anti-inflammatory activity. However, its effects on the inflammation of the cardiovascular system have not been reported yet. The aim of this study was to investigate the anti-inflammatory effects of corynoline on lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVECs). The results showed that LPS significantly increased the expression of VCAM-1 and ICAM-1. The production of cytokines TNF-α and IL-8 was also up-regulated by LPS. However, these increases were concentration-dependently suppressed by the treatment of corynoline. To investigate the anti-inflammatory mechanism of corynoline, we checked the activation of NF-κB and the expression of Nrf2. The results showed that LPS-induced NF-κB activation was suppressed by corynoline. The expression of Nrf2 and HO-1 was up-regulated by the treatment of corynoline. Knockdown of Nrf2 could reverse the anti-inflammatory effects of corynoline. In conclusion, the results indicated that corynoline exhibited anti-inflammatory activity by activating Nrf2.

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Authors and Affiliations

  1. Cardiovascular Disease Center, First Hospital of Jilin University, Changchun, Jilin Province, 130021, People’s Republic of China

    Bin Liu

  2. Department of Otolaryngology Head and Neck Surgery, Second Hospital of Jilin University, Changchun, Jilin Province, 130041, People’s Republic of China

    Kai Su

  3. Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People’s Republic of China

    Jiaxin Wang, Jingjing Wang & Yunhe Fu

  4. Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, Jilin Province, 130062, People’s Republic of China

    Zhuoyuan Xin, Fan Li & Yunhe Fu

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  1. Bin Liu
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  2. Kai Su
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  3. Jiaxin Wang
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  7. Yunhe Fu
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Correspondence to Yunhe Fu.

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Liu, B., Su, K., Wang, J. et al. Corynoline Exhibits Anti-inflammatory Effects in Lipopolysaccharide (LPS)-Stimulated Human Umbilical Vein Endothelial Cells through Activating Nrf2. Inflammation 41, 1640–1647 (2018). https://doi.org/10.1007/s10753-018-0807-6

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  • DOI: https://doi.org/10.1007/s10753-018-0807-6

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