Abstract
Periostin is essential for periodontal tissue integrity and homeostasis and also associated with periodontitis and periodontitis healing. This study aims to investigate the temporal and spatial expression of Periostin and Wnt5a/CaMKII in periodontitis and how the Wnt5a regulates Periostin through CaMKII signaling pathway in PDLCs in inflammatory environment. The experimental periodontitis mice were adopted to clarify the temporal and spatial expression of Wnt5a, CaMKII and Periostin during early periodontitis. And the Wnt5a, CaMKII and Periostin expression pattern and regulation mechanism in PDLCs were clarified in Porphyromonas gingivalis Lipopolysaccharide (P.g. LPS) induced inflammatory condition. Along with the periodontitis development, Wnt5a, CaMKII and Periostin significantly increased in periodontal ligament and partially increased in gingiva during 0 to 6 day (P < 0.05). They were involved in early periodontitis homeostasis especially in periodontal ligament tissue. Meanwhile, Wnt5a, CaMKII and Periostin were significantly decreased at 12 h (P < 0.05) and increased at 48 h (P < 0.05) in PDLCs after induced by P.g. LPS. Besides, Wnt5a significantly enhanced total CaMKII protein (P < 0.05), pCaMKII (P < 0.001) and Periostin (P < 0.001), and this could be blocked by CaMKII inhibitor KN93 (P < 0.05). In conclusions, in early periodontitis, Wnt5a/CaMKII and Periostin should be involved in maintaining periodontal homeostasis and Wnt5a could up-regulate Periostin via CaMKII pathway in inflammation, which would provide new clues for us to understand the pathogenesis of periodontitis and develop better therapeutic strategies.
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The data that support the findings of this study is available from the corresponding author upon reasonable request.
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Acknowledgements
The work was supported by grants from the National Key Research and Development Program of China (2017YFA0104800) and the Key Research and Development rogram of Sichuan Province (2020YFS0175).
Funding
The work was supported by grants from the National Key Research and Development Program of China (Grant No. 2017YFA0104800) and the Key Research and Development Program of Sichuan Province (Grant No. 2020YFS0175).
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LQ, SG and WT designed the study; LQ, LL, SW and SG performed the experiments; LQ and SG analyzed the data; LQ prepared the first draft of the paper; LQ, SG, HP, YW and WT revised the paper. All authors reviewed and approved the final version. WT and SG were guarantor. All authors agreed to be accountable for the work and to ensure that any questions relating to the accuracy and integrity of the paper was investigated and properly resolved.
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All the experimental procedures containing human periodontal ligament cell culture and animal experiments were approved by the Animal Care and Use Committee and Ethics Committee of West China college of stomatology, Sichuan University (WCHSIRB-D-2017–159).
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Qian, L., Shujuan, G., Ping, H. et al. Wnt5a up-regulates Periostin through CaMKII pathway to influence periodontal tissue destruction in early periodontitis. J Mol Histol 52, 555–566 (2021). https://doi.org/10.1007/s10735-021-09975-z
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DOI: https://doi.org/10.1007/s10735-021-09975-z