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Physiological and biochemical responses to drought stress in cultivated and Tibetan wild barley

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Abstract

Greenhouse pot experiments were conducted to investigate genotypic differences in response to drought stress between Tibetan wild barley genotypes (XZ5 and XZ150, drought-tolerant; XZ54 and XZ147, drought-sensitive) and cv ZAU3. Drought stress of 4 % soil moisture content (SMC) significantly decreased water potential (WP) and osmotic potential (OP), while increased water saturation deficit (WSD) and the bound water content (BWC) in leaves, with the least decrease/increase in XZ5, which recorded the highest levels in WP and OP but the lowest in WSD and BWC under 4 % SMC. Under 15 % SMC, when compared with control, XZ5 and XZ150 had more elevation in soluble sugar content relative to the other 3 genotypes, while XZ5 had the least increase in soluble protein. Under 4 % SMC, endogenous ABA content increased more in XZ5 and XZ150 than in the other three genotypes, but proline content increased least in XZ5. After rewatering, the transpiration rate increased in sensitive genotypes but decreased in tolerant genotypes. The less elevated MDA accumulation was observed in XZ5 and XZ150 with higher POD and CAT activities under 15 and 4 % SMC than in the other three genotypes. Drought stress of 4 % SMC significantly up-regulated the expression levels of CAT1 and Cu/ZnSOD in XZ5 and MnSOD in XZ150.Our results indicated that drought tolerance of wild barley XZ5 is mainly associated with the osmo-regulation of soluble sugar and stomatal regulation of ABA. This mechanism could be applied to improve the drought tolerance of cultivated barley and the further marker-assisted breeding.

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References

  • Apel KH, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399

    Article  CAS  PubMed  Google Scholar 

  • Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59(2):206–216

    Article  CAS  Google Scholar 

  • Ashraf M, Ahmad A, McNeilly T (2001) Growth and photosynthetic characteristics in pearl millet under water stress and different potassium supply. Photosynthetica 39(3):389–394

    Article  CAS  Google Scholar 

  • Bornare SS, Prasad LC, Prasad R, Lal JP (2012) Perspective of barley drought tolerance; methods and mechanisms comparable to other cereals. J Progress Agric 3:68–70

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Ceccarelli S (2010) Drought and drought resistance. Encycl Biotechnol Agric Food 1:205–207

    Google Scholar 

  • Centritto M (2005) Photosynthetic limitations and carbon partitioning in cherry in response to water deficit and elevated [CO2]. Agric Ecosyst Environ 106(2–3):233–242

    Article  CAS  Google Scholar 

  • Cochard H, Coll L, Le Roux X, Ameglio T (2002) Unraveling the effects of plant hydraulics on stomatal closure during water stress in walnut. Plant Physiol 128(1):282–290

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Colom MR, Vazzana C (2003) Photosynthesis and PSII functionality of drought-resistant and drought-sensitive weeping lovegrass plants. Environ Exp Bot 49(2):135–144

    Article  CAS  Google Scholar 

  • Cornic G, Fresneau C (2002) Photosynthetic carbon reduction and carbon oxidation cycles are the main electron sinks for photosystem II activity during a mild drought. Ann Bot 89:887–894

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Crozier A, Sandberg G, Monteiro AM, Sundberg B (1986) The use of immunological techniques in plant hormone analysis. In: Bopp M (ed)   Plant growth substances 1985. Springer, Berlin, pp 13–21

  • de Souza TC, Magalhães PC, de Castro EM, Carneiro NP, Padilha FA, Júnior CCG (2014) ABA application to maize hybrids contrasting for drought tolerance: changes in water parameters and in antioxidant enzyme activity. Plant Growth Regul 73(3):205–217

    Article  Google Scholar 

  • Desikan R, Cheung MK, Bright J, Henson D, Hancock JT, Neill SJ (2004) ABA, hydrogen peroxide and nitric oxide signaling in stomatal guard cells. J Exp Bot 55:205–212

    Article  CAS  PubMed  Google Scholar 

  • Ellis RP, Forster BP, Robinson D, Handley LL, Gordon DC, Russell JR, Powell W (2000) Wild barley: a source of genes for crop improvement in the 21st century? J Exp Bot 342(51):9–17

    Article  Google Scholar 

  • Flexas J, Bota J, Loreto F, Cornic G, Sharkey TD (2004) Diffusive and metabolic limitations to photosynthesis under drought and sanlinity in C3 plants. Plant Biol 6:269–279

    Article  CAS  PubMed  Google Scholar 

  • Foyer CH, Noctor G (2005) Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant, Cell Environ 28:1056–1071

    Article  CAS  Google Scholar 

  • Gupta N, Thind SK, Bains NS (2014) Glycine betaine application modifies biochemical attributes of osmotic adjustment in drought stressed wheat. Plant Growth Regul 72(3):221–228

    Article  CAS  Google Scholar 

  • Hartung W, Sauter A, Hose E (2002) Abscisic acid in the xylem: where does it come from, where does it go to? J Exp Bot 53:27–37

    Article  CAS  PubMed  Google Scholar 

  • Hong ZL, Lakkineni K, Zhang ZM, Verma DPS (2000) Removal of feedback inhibition of Δ1-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiol 122:1129–1136

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kaur K, Kaur N, Gupta AK, Singh I (2013) Exploration of the antioxidative defense system to characterize chickpea genotypes showing differential response towards water deficit conditions. Plant Growth Regul 70(1):49–60

    Article  CAS  Google Scholar 

  • Kautz B, Noga G, Hunsche M (2014) Sensing drought-and salinity-imposed stresses on tomato leaves by means of fluorescence techniques. Plant Growth Regul 73(3):279–288

    Article  CAS  Google Scholar 

  • Krause GH, Wei E (1991) Chlorophyll fluorescence and photosynthesis: the basics. Ann Rev Plant Physiol Plant Mol Biol 42:313–349

    Article  CAS  Google Scholar 

  • Liang M, Chen D, Lin M, Zheng Q, Huang Z, Lin Z, Zhao G (2014) Isolation and characterization of two DREB1 genes encoding dehydration-responsive element binding proteins in chicory (Cichorium intybus). Plant Growth Regul 73(1):45–55

    Article  CAS  Google Scholar 

  • Nayyar H, Walia DP (2003) Water stress induced proline accumulation in contrasting wheat genotypes as affected by calcium and abscisic acid. Biol Plant 46:275–279

    Article  CAS  Google Scholar 

  • Pennisi E (2008) The blue revolution, drop by drop, gene by gene. Science 320:171–173

    Article  CAS  PubMed  Google Scholar 

  • Pinheiro HA, DaMatta FM, Chaves ARM, Fontes EPB, Loureiro ME (2004) Drought tolerance in relation to protection against oxidative stress in clones of Coffea canephora subjected to long-term drought. Plant Sci 167(6):1307–1314

    Article  CAS  Google Scholar 

  • Ranney TG, Bassuk NL, Whitlow TH (1991) Osmotic adjustment and solute constituents in leaves and roots of water-stressed cherry (Prunus) trees. J Am Soc Hortic Sci 116(4):684–688

    CAS  Google Scholar 

  • Saradadevi R, Bramley H, Siddique KHM, Edwards E, Palta JA (2014) Contrasting stomatal regulation and leaf ABA concentrations in wheat genotypes when split root systems were exposed to terminal drought. Field Crop Res 162:77–86

    Article  Google Scholar 

  • Singh T, Paleg I, Aspinall D (1973) Stress metabolism I. Nitrogen metabolism and growth in the barley plant during water stress. Aust J Biol Sci 26:45–56

    CAS  Google Scholar 

  • Song XS, Hu WH, Mao WH, Ogweno JO, Zhou YH, Yu JQ (2005) Response of ascorbate peroxidase isoenzymes and ascorbate regeneration system to abiotic stresses in Cucumis sativus L. Plant Physiol Biochem 43(12):1082–1088

    Article  CAS  PubMed  Google Scholar 

  • Szalai G, Kellős T, Galiba G, Kocsy G (2009) Glutathione as an antioxidant and regulatory molecule in plants under abiotic stress conditions. J Plant Growth Regul 28(1):66–80

    Article  CAS  Google Scholar 

  • Tang QY, Feng MG (2002) DPS data processing system for practical statistics. Science Press, Bejing

    Google Scholar 

  • Wang W, Vinocur B, Shoseyov O, Altman A (2004) Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci 9(5):244–252

    Article  CAS  PubMed  Google Scholar 

  • Wang A, Yu Z, Ding Y (2009) Genetic diversity analysis of wild close relatives of barley from Tibet and the Middle East by ISSR and SSR markers. C R Biol 332(4):393–403

    Article  CAS  PubMed  Google Scholar 

  • Wu FB, Zhang GP, Dominy P (2003) Four barley genotypes respond differently to cadmium: lipid peroxidation and activities of antioxidant capacity. Environ Exp Bot 50(1):67–78

  • Yemm EW, Willis AJ (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57:508–514

    PubMed Central  CAS  PubMed  Google Scholar 

  • Zhang XZ (1992) The measurement and mechanism of lipid peroxidation and SOD, POD and CAT activities in biological system. In: Zhang XZ (ed) Research methodology of crop physiology. Agriculture Press, Beijing, pp 208–211

    Google Scholar 

  • Zhang X, Zhang L, Dong FC, Gao JF, Galbraith DW, Song CP (2001) Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba. Plant Physiol 126:1438–1448

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhao J, Sun HY, Dai HX, Zhang GP, Wu FB (2010) Difference in response to drought stress among Tibet wild barley genotypes. Euphytica 172(3):395–403

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Crop Science, Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Mian Zhang, Jing Zhao, Guoping Zhang & Feibo Wu

  2. Cixi Research Institute of Agricultural Science, Cixi, 315300, People’s Republic of China

    Zhu-Qun Jin

Authors
  1. Mian Zhang
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  2. Zhu-Qun Jin
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  3. Jing Zhao
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  4. Guoping Zhang
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  5. Feibo Wu
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Corresponding author

Correspondence to Feibo Wu.

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Mian Zhang and Zhu-Qun Jin have contributed equally to this article.

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Zhang, M., Jin, ZQ., Zhao, J. et al. Physiological and biochemical responses to drought stress in cultivated and Tibetan wild barley. Plant Growth Regul 75, 567–574 (2015). https://doi.org/10.1007/s10725-014-0022-x

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  • DOI: https://doi.org/10.1007/s10725-014-0022-x

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