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Difference in response to drought stress among Tibet wild barley genotypes

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Abstract

Wild barley germplasms are a treasure trove of useful genes and provide rich sources of genetic variation for crop improvement. We carried out a hydroponic and pot evaluation of diverse germplasms in response to drought stress using 47 Tibet annual wild barley genotypes. Our results from the hydroponic experiment showed that SPAD (soil–plant analyses development analyses, based on chlorophyll meter readings), plant height, and biomass of shoot/root were significantly reduced in plants exposed to drought stress (exposure of plant roots to air for 6 h daily for a period of 7 days) compared with control plants. There were significant differences among the 47 wild barley genotypes in terms of the reduction in these growth parameters, with variation coefficients and diversity indexes ranging from 23.2 to 49.1% and 1.46 to 1.62, respectively, suggesting a rich genetic diversity among the tested accessions. Genotypes XZ5 and XZ150 showed the least reduction, indicating their high tolerance to drought stress, while genotypes XZ54 and XZ147 showed the greatest reduction, and drought stress symptoms appeared rapidly and severely in these latter genotypes. The trends in the different responses of these genotypes to drought stress were fairly consistent in both the hydroponic and the two pot selection experiments, with XZ5 and XZ150 screened as drought-resistant genotypes and XZ54, XZ147 as drought-sensitive ones. Significant genotypic differences in leaf soluble sugar content and water use efficiency in response to drought stress were also observed in pot experiment two, with the drought-tolerant genotype XZ5 showing a markedly higher recovery and the two drought-sensitive genotypes XZ54 and XZ147 showing a markedly lower recovery than the control plants.

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Abbreviations

Ca:

Ambient CO2 molar ratio

CV:

Coefficient of variation

DW:

Dry weight

FW:

Fresh weight

ICARDA:

International Center for Agricultural Research in the Dry Areas

PPFD:

Photosynthetic photo flux density

SG:

Sensitive genotype

SPAD:

Soil–plant analyses development

TG:

Tolerant genotype

WUE:

Water use efficiency

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Acknowledgements

Financial support from Zhejiang Provincial Natural Science Foundation for Outstanding Young Scientists (R306202), the Special Foundation for the Author of National Excellent Doctoral Dissertation of China (FANEDD 200556), and the National Natural Science Foundation of China (30630047) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Huajiachi Campus, Hangzhou, 310029, China

    Jing Zhao, Hongyan Sun, Huaxin Dai, Guoping Zhang & Feibo Wu

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  1. Jing Zhao
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  2. Hongyan Sun
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  3. Huaxin Dai
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  4. Guoping Zhang
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  5. Feibo Wu
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Correspondence to Feibo Wu.

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Zhao, J., Sun, H., Dai, H. et al. Difference in response to drought stress among Tibet wild barley genotypes. Euphytica 172, 395–403 (2010). https://doi.org/10.1007/s10681-009-0064-8

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  • DOI: https://doi.org/10.1007/s10681-009-0064-8

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