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Reactive oxygen species localization in roots of Arabidopsis thaliana seedlings grown under phosphate deficiency

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Abstract

Arabidopsis plants responding to phosphorus (P) deficiency increase lateral root formation and reduce primary root elongation. In addition the number and length of root hairs increases in response to P deficiency. Here we studied the patterns of radical oxygen species (ROS) in the roots of Arabidopsis seedlings cultured on media supplemented with high or low P concentration. We found that P availability affected ROS distribution in the apical part of roots. If plants were grown on high P medium, ROS were located in the root elongation zone and quiescent centre. At low P ROS were absent in the elongation zone, however, their synthesis was detected in the primary root meristem. The proximal part of roots was characterized by ROS production in the lateral root primordia and in elongation zones of young lateral roots irrespective of P concentration in the medium. On the other hand, plants grown at high or low P differed in the pattern of ROS distribution in older lateral roots. At high P, the elongation zone was the primary site of ROS production. At low P, ROS were not detected in the elongation zone. However, they were present in the proximal part of the lateral root meristem. These results suggest that P deficiency affects ROS distribution in distal parts of Arabidopsis roots. Under P-sufficiency ROS maximum was observed in the elongation zone, under low P, ROS were not synthesized in this segment of the root, however, they were detected in the apical root meristem.

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Abbreviations

DCF:

2′,7′-Dichlorofluorescein

DCFH:

2′,7′-Dichlorodihydrofluorescein

NBT:

Nitroblue tetrazolium

QC:

Quiescent centre

ROS:

Radical oxygen species

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Acknowledgments

This work was financially supported by the Grant of the Rector of Nicolaus Copernicus University (Grant No. 525-B).

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Authors and Affiliations

  1. Department of Biotechnology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100, Toruń, Poland

    Jarosław Tyburski, Kamila Dunajska & Andrzej Tretyn

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  1. Jarosław Tyburski
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  2. Kamila Dunajska
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  3. Andrzej Tretyn
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Correspondence to Jarosław Tyburski.

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Tyburski, J., Dunajska, K. & Tretyn, A. Reactive oxygen species localization in roots of Arabidopsis thaliana seedlings grown under phosphate deficiency. Plant Growth Regul 59, 27–36 (2009). https://doi.org/10.1007/s10725-009-9385-9

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  • DOI: https://doi.org/10.1007/s10725-009-9385-9

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