Abstract
Reactive oxygen species (ROS) is a chemically reactive chemical substance containing oxygen and a natural by-product of normal oxygen metabolism. Excessive ROS affect the growth process of crops, which will lead to the decrease of yield. Nitrogen, as a critical nutrient element in plants and plays a vital role in plant growth and crop production. Nitrate is the primary nitrogen source available to plants in agricultural soil and various natural environments. However, the molecular mechanism of ROS-nitrate crosstalk is still unclear. In this study, we used the foxtail millet (Setaria italica L.) as the material to figure it out. Here, we show that excessive NaCl inhibits nitrate-promoted plant growth and nitrogen use efficiency (NUE). NaCl induces ROS accumulation in roots, and ROS inhibits nitrate-induced gene expression in a short time. Surprisingly, low concentration ROS slight promotes and high concentration of ROS inhibits foxtail millet growth under long-term H2O2 treatment. These results may open a new perspective for further exploration of ROS-nitrate signaling pathway in plants.
Key message
Inhibition of nitrogen use efficiency under stress environments may be caused by compromise of ROS-mediated nitrogen signal transduction.
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Acknowledgements
This work was supported by National Natural Science Foundation (32200222 for J. W); High-level Talents Start-up Fund of Shanxi Agricultural University (J242198006 for J. W); Start-up Fund of Shanxi Agricultural University (2021BQ84 for X. C); Shanxi Province Outstanding Doctoral and Post-Doctoral Scholarship Award Foundation (SXBYKY2021055 for J. W, SXBYKY2022033 for X. C, and SXBYKY2021059 for G.Y) and Hou Ji Laboratory Foundation (202204010910001-32 for J. W).
Funding
This work was supported by National Natural Science Foundation (32200222 for J. W); High-level Talents Start-up Fund of Shanxi Agricultural University (J242198006 for J. W); Start-up Fund of Shanxi Agricultural University (2021BQ84 for X. C); Shanxi Province Outstanding Doctoral and Post-Doctoral Scholarship Award Foundation (SXBYKY2021055 for J. W, SXBYKY2022033 for X. C, and SXBYKY2021059 for G.Y) and Hou Ji Laboratory Foundation (202204010910001-32 for J. W).
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Meng, HX., Wang, YZ., Yao, XL. et al. Reactive oxygen species (ROS) modulate nitrogen signaling using temporal transcriptome analysis in foxtail millet. Plant Mol Biol 114, 37 (2024). https://doi.org/10.1007/s11103-024-01435-y
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DOI: https://doi.org/10.1007/s11103-024-01435-y