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Conferring resistance to pre-harvest sprouting in durum wheat by a QTL identified in Triticum spelta

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Abstract

Pre-harvest sprouting (PHS) causes significant yield loss and degrade the end-use quality of wheat, especially in regions with prolonged wet weather during the harvesting season. Unfortunately, the gene pool of Triticum durum (tetraploid durum wheat) has narrow genetic base for PHS resistance. Therefore, finding out new genetic resources from other wheat species to develop PHS resistance in durum wheat is of importance. A major PHS resistance QTL, Qphs.sicau-3B.1, was mapped on chromosome 3BL in a recombinant inbred line population derived from ‘CSCR6’ (Triticum spelta), a PHS resistant hexaploid wheat and ‘Lang’, a PHS susceptible Australian hexaploid wheat cultivar. This QTL, Qphs.sicau-3B.1, is positioned between DArT marker wPt-3107 and wPt-6785. Two SCAR markers (Ph3B.1 and Ph3B.2) were developed to track this major QTL and were used to assay a BC2F8 tetraploid population derived from a cross between the durum wheat ‘Bellaroi’ (PHS susceptible) and ‘CSCR6’ (PHS resistant). Phenotypic assay and marker-assisted selection revealed five stable tetraploid lines were highly PHS resistant. This study has successfully established that PHS-resistance QTL from hexaploid wheat could be efficiently introgressed into tetraploid durum wheat. This tetraploid wheat germplasm could be useful in developing PHS resistant durum cultivars with higher yield and good end-use quality.

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Acknowledgements

This work was supported by the National Basic Research Program of China (2014CB147200), the National Natural Science Foundation of China (31171555, 31230053, and 31571654), and Research Fund for the Doctoral Program of Higher Education of China (20135103110008).

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    Authors and Affiliations

    1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China

      Yujiao Liu, Yaxi Liu, Yong Zhou, Zhien Pu, Pengfei Qi, Qiantao Jiang, Mei Deng, Zaoxia Wang, Yuming Wei, Dengcai Liu, Youliang Zheng & Jirui Wang

    2. Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, Ottawa, ON, K1A 0C6, Canada

      Yujiao Liu, Charlene Wight, Wenguang Cao & Judith Frégeau-Reid

    3. CSIRO Agriculture Flagship, 306 Carmody Road, St Lucia, QLD, 4067, Australia

      Chunji Liu

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    1. Yujiao Liu
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    Correspondence to Jirui Wang.

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    Yujiao Liu and Yaxi Liu have contributed equally for this work.

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    Liu, Y., Liu, Y., Zhou, Y. et al. Conferring resistance to pre-harvest sprouting in durum wheat by a QTL identified in Triticum spelta . Euphytica 213, 19 (2017). https://doi.org/10.1007/s10681-016-1796-x

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