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Identification and validation of a major QTL conferring crown rot resistance in hexaploid wheat

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Abstract

Crown rot (CR), caused by various Fusarium species, is a chronic wheat disease in Australia. As part of our objective of improving the efficiency of breeding CR resistant wheat varieties, we have been searching for novel sources of resistance. This paper reports on the genetic control of one of these newly identified resistant genotypes, ‘CSCR6’. A population derived from a cross between CSCR6 and an Australian variety ‘Lang’ was analyzed using two Fusarium isolates belonging to two different species, one Fusarium pseudograminearum and the other Fusarium graminearum. The two isolates detected QTL with the same chromosomal locations and comparable magnitudes, indicating that CR resistance is not species-specific. The resistant allele of one of the QTL was derived from ‘CSCR6’. This QTL, designated as Qcrs.cpi-3B, was located on the long arm of chromosome 3B and explains up to 48.8% of the phenotypic variance based on interval mapping analysis. Another QTL, with resistant allele from the variety ‘Lang’, was located on chromosome 4B. This QTL explained up to 22.8% of the phenotypic variance. A strong interaction between Qcsr.cpi-3B and Qcsr.cpi-4B was detected, reducing the maximum effect of Qcrs.cpi-3B to 43.1%. The effects of Qcrs.cpi-3B were further validated in four additional populations and the presence of this single QTL reduced CR severity by up to 42.1%. The fact that significant effects of Qcrs.cpi-3B were detected across all trials with different genetic backgrounds and with the use of isolates belonging to two different Fusarium species make it an ideal target for breeding programs as well as for further characterization of the gene(s) involved in its resistance.

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Acknowledgments

JM is grateful to the University of Western Australia for financial support through an International Postgraduate Research Scholarship and the University Postgraduate Awards, CYZ to the Service Centre for Experts and Scholars of Hebei Province, China, and YXL to the China Scholarship Council. The research reported in this paper was partially funded by the Grains Research and Development Corporation (GRDC).

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Authors and Affiliations

  1. CSIRO Plant Industry, 306 Carmody Road, St Lucia, QLD, 4067, Australia

    J. Ma, H. B. Li, C. Y. Zhang, X. M. Yang, Y. X. Liu & C. J. Liu

  2. School of Plant Biology, The University of Western Australia, Perth, WA, 6009, Australia

    J. Ma, G. J. Yan & C. J. Liu

  3. College of Life Science, Agricultural University of Hebei, Hebei, People’s Republic of China

    C. Y. Zhang

  4. Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, People’s Republic of China

    X. M. Yang

  5. Key Laboratory of Crop Genetic Resource and Improvement, Ministry of Education Sichuan Agricultural University, Yaan, 625014, Sichuan, People’s Republic of China

    Y. X. Liu

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  1. J. Ma
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  2. H. B. Li
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Correspondence to C. J. Liu.

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Communicated by G. Bryan.

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Ma, J., Li, H.B., Zhang, C.Y. et al. Identification and validation of a major QTL conferring crown rot resistance in hexaploid wheat. Theor Appl Genet 120, 1119–1128 (2010). https://doi.org/10.1007/s00122-009-1239-3

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  • DOI: https://doi.org/10.1007/s00122-009-1239-3

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