Abstract
Osteochondral grafts are suitable materials for repair of articular cartilage defect and plastic and reconstructive surgery. In our study, osteochondral allografts from rabbits were preserved in vitro for 28 days, and chondrocyte death, degradation of collagen and proteoglycan, morphological alterations, and inflammatory reaction were observed in the grafts. Supplementing of Aucubin with 10 or 20 μM in the preservation solution inhibited chondrocyte death, matrix degradation, pathological alterations and inflammation in allografts preserved in vitro, compared with that preserved in standard preservation solution. In addition, after transplantation of 20 μM Aucubin-treated allografts, the osteochondral repair and regeneration of rabbits with knee joint defect were accelerated. In conclusion, Aucubin was beneficial for maintaining chondrocyte viability and normal morphology, and inhibiting inflammatory occurrence in rabbit osteochondral grafts preserved in vitro, and facilitated osteochondral repair and regeneration of rabbits with knee defect. These findings might provide novel insights for preservation of grafts for clinical articular cartilage repair and plastic surgery.
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Abbreviations
- TNF:
-
Tumor necrosis factor
- FDA:
-
Fluorescein diacetate
- EB:
-
Ethidium bromide
- HE:
-
Hematoxylin eosin
- RIPA:
-
Radio immunoprecipitaion
- PMSF:
-
Phenylmethanesulfnyl fluoride
- SDS-PAGE:
-
Sodium dodecyl sulfate–polyacrylamide gelelectrophoresis
- PVDF:
-
Vinylidene fluoride
- GAPDH:
-
Glyceraldehyde-3-phosphate dehydrogenase
- TBST:
-
Tris Buffered Saline with Tween
- ECL:
-
Electrochemi luminescence
- HRP:
-
Horse radish peroxidase
- M-MLV:
-
Moloney murine leukemia virus
- SD:
-
Standard deviation
- IL:
-
Interleukin
- NFκB:
-
Nuclear factor kappa-B
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GZ and HJ designed the study together. GZ performed most of experiments, analyzed data and drafted the manuscript. HJ modified the manuscript. All authors approved the submission.
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Zhao, G., Jiang, H. Beneficial effects of Aucubin on restoration of rabbits with cartilage defect. Cell Tissue Bank 23, 887–897 (2022). https://doi.org/10.1007/s10561-022-10004-w
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DOI: https://doi.org/10.1007/s10561-022-10004-w