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An efficient method for CTCs screening with excellent operability by integrating Parsortix™-like cell separation chip and selective size amplification

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Abstract

In this article, an attempt for efficient screening of circulating tumor cells (CTCs) with excellent operability on microfluidic chips was reported. A Parsortix™-like cell separation chip was manufactured in our lab. This chip allowed lateral flow of fluid which increased the flow rate of blood. And, an air valve controlled injection pump was manufactured which allowed eight chips working simultaneously. This greatly facilitated the blood treatment process and saved time. As for the mechanism of screening circulating tumor cells, selective size amplification was utilized. By size amplification of cancer cells, both the hardness and the size of CTCs increased which differentiated them from blood cells. And the modification procedure of beads used for size amplification of cancer cells was optimized. Finally, by integrating the commercialized Parsortix™-like cell separation chip and selective size amplification, a practical method for screening circulating tumor cells was established.

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Acknowledgements

This research was supported by the National High-tech R&D Program of China (863 Program 2015AA020401), the National Basic Research Program (2015CB931801) and the National Natural Science Foundation of China (51690151, 51473093).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China

    Xin Jin, Rui Chen, Bai Xue & Xinyuan Zhu

  2. State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren’ai Road, Suzhou, 215123, China

    Rui Chen

  3. National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Department of Micro/Nano Electronics, Shanghai Jiao Tong University, Shanghai, 200240, China

    Shikun Zhao & Xiang Chen

  4. Department of Nuclear Medicine, and Department of Gastrointestinal Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China

    Peiyong Li

Authors
  1. Xin Jin
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  2. Rui Chen
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  3. Shikun Zhao
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  4. Peiyong Li
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  5. Bai Xue
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Correspondence to Rui Chen or Xiang Chen.

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Jin, X., Chen, R., Zhao, S. et al. An efficient method for CTCs screening with excellent operability by integrating Parsortix™-like cell separation chip and selective size amplification. Biomed Microdevices 20, 51 (2018). https://doi.org/10.1007/s10544-018-0293-5

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