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Acclimatization of tissue cultured plantlets: from laboratory to land

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Abstract

The ultimate success of micropropagation on a commercial scale depends on the ability to transfer plants out of culture on a large scale, at low cost and with high survival rates. During field transfer the in vitro grown plantlets are unable to compete with soil microbes and to cope with the environmental conditions. The in vitro culture conditions result in the plantlets with altered morphology, anatomy and physiology. In order to increase growth and reduce mortality in plantlets at the acclimatisation stage, efforts are focused on the control of both physical and chemical environment and biohardening of micropropagated plantlets. This review describes the abiotic and biotic stresses and current developing methods for the acclimatization of microshoots.

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Acknowledgments

SC is highly thankful for the financial support from University Grants Commission (UGC), GOI, New Delhi for the major project [F. No. 37-111/2009 (SR)]. The authors are thankful to the Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi, for providing infrastructure facilities. SC also wishes to thank Dr. M. M. Sharma (Department of Botany, Rajasthan University) for his support. Authors also thank M. Senthilkumar (Horticultural College and Research Institute, Tamilnadu Agricultural University) for his permission to use the photographs in this article.

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Authors and Affiliations

  1. Birla Institute of Technology, Mesra, Ranchi, Jharkhand, 835215, India

    Sheela Chandra, Rajib Bandopadhyay, Vijay Kumar & Ramesh Chandra

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  1. Sheela Chandra
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  2. Rajib Bandopadhyay
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  3. Vijay Kumar
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  4. Ramesh Chandra
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Correspondence to Sheela Chandra.

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Chandra, S., Bandopadhyay, R., Kumar, V. et al. Acclimatization of tissue cultured plantlets: from laboratory to land. Biotechnol Lett 32, 1199–1205 (2010). https://doi.org/10.1007/s10529-010-0290-0

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  • DOI: https://doi.org/10.1007/s10529-010-0290-0

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