Abstract
An aluminium hydroxide adjuvant induced a more elevated and rapid immune responses against short peptides conjugated to the Keyhole Lympet Hemocyanin carrier than immuneasy adjuvant. Furthermore, since carrier proteins may compete with the fused or chemically linked polypeptides in eliciting antigen-specific immune response, we classified the immunogenicity of the most common carrier proteins used in molecular biology for antigen expression and mouse immunisation. The disulfide isomerase protein A gave a carrier with the lowest immunogenicity whilst disulfide isomerase protein C gave the highest immunogenicity and therefore should be avoided as a fusion partner. Using this protein as a model, we identified and located the immunodominant epitopes along its sequence. These results now enable the combination of carrier and immunisation conditions to be optimized.
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Acknowledgments
The authors wish to thank N. Rosenthal and C. Boulin for having made accessible the EMBL Facilities and M. Leuener and C. Fasci for technical assistance. PC is supported by FIRB-Idee Progettuali RBIP0695BB_001.
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Purpose of work
Short-peptide immunisation remains a trial-and-error procedure; however, single components of the protocol, like the rational choice of carriers and adjuvants that can influence the animal immune response, can be optimized.
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Chiarella, P., Edelmann, B., Fazio, V.M. et al. Antigenic features of protein carriers commonly used in immunisation trials. Biotechnol Lett 32, 1215–1221 (2010). https://doi.org/10.1007/s10529-010-0283-z
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DOI: https://doi.org/10.1007/s10529-010-0283-z