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Biocontrol potential of the antagonistic microorganism Streptomyces enissocaesilis against Orobanche cumana

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Abstract

The root parasitic weed Orobanche cumana (sunflower broomrape) constrains sunflower (Helianthus annuus L.) production in many areas of the world. A seed germination experiment, a co-culture experiment, and a pot experiment were conducted to study the effect of some soilborne antagonistic microorganisms on O. cumana. Results showed that culture filtrate of Streptomyces enissocaesilis significantly reduced the germination rate of O. cumana seeds both in the seed germination experiment and the co-culture experiment, with more than 50 and 40 % (after cultivation for eight days) reduction respectively over the control. In the pot experiment, applying S. enissocaesilis reduced the epigaeous number of O. cumana tubercles by 47.5 % after 130 days. Furthermore, S. enissocaesilis also increased polyphenol oxidase activity in sunflower roots and improved the beneficial microflora in the rhizosphere soil of sunflower. Selecting biocontrol agents from soilborne antagonistic microorganisms might be a promising strategy for controlling O. cumana.

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Acknowledgments

The authors thank the National Natural Science Foundation of China (31360505) and the National Science and Technology Support Program (2011BAD31B05) for the financial support of this work. Special acknowledgements are given to the editor and the two reviewers for their careful edits and valuable comments which improved our manuscript.

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Authors and Affiliations

  1. College of Natural Resources and Environment, Northwest A&F University, Yangling, 712100, Shaanxi, China

    J. Chen, Q. H. Xue & X. T. Jia

  2. School of Chemistry, University of Sydney, Sydney, NSW, 2006, Australia

    C. S. P. McErlean

  3. College of Plant Science, Tarim University, Alar City, 843300, Xinjiang Uyghur Autonomous Region, China

    J. H. Zhi

  4. The State Key Laboratory of Soil Erosion and Dryland Farming, Institute of Soil and Water Conservation, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Y. Q. Ma

  5. Institute of Soil and Water Conservation, Chinese Academy of Sciences, Yangling, 712100, Shaanxi, China

    M. Zhang

  6. College of Forestry, Northwest A&F University, Yangling, 712100, Shaanxi, China

    X. X. Ye

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  1. J. Chen
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Correspondence to Y. Q. Ma.

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Handling Editor: S. Raghu.

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Chen, J., Xue, Q.H., McErlean, C.S.P. et al. Biocontrol potential of the antagonistic microorganism Streptomyces enissocaesilis against Orobanche cumana . BioControl 61, 781–791 (2016). https://doi.org/10.1007/s10526-016-9738-z

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  • DOI: https://doi.org/10.1007/s10526-016-9738-z

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