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Streptococcosis in Oreochromis sp.: is feed-based biofilm vaccine of Streptococcus agalactiae effective?

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Abstract

This study was carried out to determine the potential of a biofilm vaccine of Streptococcus agalactiae in protecting tilapia from streptococcocal infection. The tilapia were vaccinated orally using the biofilm vaccine with 1091010 CFU/g (FBV) incorporated in feed and killed whole-cell vaccine (FCV) of S. agalactiae also prepared in feed, and the unvaccinated control group (Cx) was fed with standard feed formulation. Throughout the study, serum, mucus and gut lavage fluid were collected to evaluate the antibody responses among the groups. The results showed that tilapia vaccinated with FBV exhibited significantly (p < 0.05) stronger immune response and high protection with the relative percentage survival (RPS) of more than 70% (85%) compared with the FCV group, which was only 56%. None of the tilapia in the Cx group survived (0%) post-challenge. Thus, the study revealed that the feed-based biofilm vaccine (FBV) significantly confers the immune protection against the high-dose challenge of S. agalactiae by secretion of the protective immunity in the mucosal and humoral in tilapia.

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Acknowledgement

We would like to thank the farmer involved in this study and staff of Aquaculture Experimental Station, Faculty of Agriculture, UPM, Puchong, Selangor, for their excellent technical assistance.

Funding

This project was funded by the Ministry of Science, Technology and Innovation, Malaysia (MOSTI) under the vote no. 5450652.

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Authors and Affiliations

  1. Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    M. Kahieshesfandiari & M. Y. Ina-salwany

  2. Deparment of Veterinary Pathology & Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    M. Y. Sabri, O. Noraini, A. A. Ajadi & A. I. Isiaku

  3. Department of Veterinary Clinical, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    M. D. Hassan

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  1. M. Kahieshesfandiari
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  2. M. Y. Sabri
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  3. M. Y. Ina-salwany
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  4. M. D. Hassan
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  5. O. Noraini
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  7. A. I. Isiaku
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Correspondence to M. Y. Sabri.

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All applicable institutional guidelines for the care and use of animals were followed by the authors.

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Kahieshesfandiari, M., Sabri, M.Y., Ina-salwany, M.Y. et al. Streptococcosis in Oreochromis sp.: is feed-based biofilm vaccine of Streptococcus agalactiae effective?. Aquacult Int 27, 817–832 (2019). https://doi.org/10.1007/s10499-019-00372-8

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