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Runella soli sp. nov., isolated from garden soil

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Abstract

A Gram-stain negative, aerobic, salmon-pink, non-motile and rod-shaped bacterium, designated strain 15J11-1T, was isolated from a soil sample collected from the university garden in Nowongu, South Korea. The 16S rRNA gene sequence analysis showed that strain 15J11-1T is phylogenetically related to Runella slithyformis DSM 19594T and Runella palustris HMF3829T (96.9% and 95.4% sequence similarity, respectively). The major fatty acids of strain 15J11-1T were identified as iso-C15:0, iso-C17:0 3-OH, C16:1ω5c and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The predominant respiratory quinone was identified as MK-7. The polar lipids were found to comprise of phosphatidylethanolamine, three unidentified aminolipids, five unidentified glycolipids, two unidentified aminoglycolipids, an unidentified phospholipid and an unidentified polar lipid. The G + C content in the genomic DNA of the strain 15J11-1T was determined to be 49.9 mol%. Based on the results of genotypic, phenotypic and chemotaxonomic analyses, strain 15J11-1T is concluded to represent a novel species of the genus Runella, for which the name Runella soli sp. nov. (type strain 15J11-1T = KCTC 52021T = NBRC 112817T) is proposed.

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Acknowledgements

This work was supported by a research grant from Seoul Women’s University (2018) and the Project on Survey of Indigenous Species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) (NIBR201701206). We thank Prof Dr. Bernhard Schink (University of Konstanz, Konstanz, Germany) for the species name.

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Authors and Affiliations

  1. Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea

    Geeta Chhetri, Jiyoun Kim, Inhyup Kim & Taegun Seo

  2. Department of Bio and Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 01797, South Korea

    Myung Kyum Kim

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  1. Geeta Chhetri
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  2. Jiyoun Kim
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  3. Inhyup Kim
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  4. Myung Kyum Kim
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Correspondence to Taegun Seo.

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Chhetri, G., Kim, J., Kim, I. et al. Runella soli sp. nov., isolated from garden soil. Antonie van Leeuwenhoek 112, 1245–1252 (2019). https://doi.org/10.1007/s10482-019-01257-9

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