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Microbispora tritici sp. nov., a novel actinomycete isolated from a root of wheat (Triticum aestivum L.)

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Abstract

A novel actinomycete, designated strain NEAU-HRGS1-13T, was isolated from a root of wheat (Triticum aestivum L.) and characterised using a polyphasic approach. Morphological and chemotaxonomic characteristics were consistent with those of members of the genus Microbispora. The major menaquinones were identified as MK-9(H2) and MK-9(H4) and the whole cell hydrolysates found to contain meso-diaminopimelic acid and madurose. The phospholipid profile was found to consist of diphosphatidylglycerol, a ninhydrin-positive glycophospholipid, phosphatidylinositol mannosides, phosphatidylmonomethylethanolamine, an unidentified glycolipid and an unidentified lipid. The major fatty acids were identified as iso-C16:0, C16:0, 10-methyl C17:0, C18:0 and C17:0. The 16S rRNA gene sequence analysis showed that the isolate is closely related to Microbispora triticiradicis NEAU-HRDPA2-9T (99.4%), Microbispora bryophytorum NEAU-TX2-2T (99.0%), Microbispora camponoti 2C-HV3T (98.8%), Microbispora hainanensis DSM 45428T (98.8%), Microbispora amethystogenes JCM 3021T (98.6%), Microbispora siamensis NBRC 104113T (98.5%), Microbispora corallina JCM 10267T (98.3%) and Microbispora rosea subsp. rosea JCM 3006T (98.2%). However, DNA–DNA relatedness and cultural, physiological and biochemical data showed that strain NEAU-HRGS1-13T can be distinguished from its close relatives. Therefore, it is concluded that strain NEAU-HRGS1-13T represents a novel species of the genus Microbispora, for which the name Microbispora tritici sp. nov. is proposed. The type stain is NEAU-HRGS1-13T (= CGMCC 4.7402T = DSM 104650T).

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Acknowledgements

We are grateful to Professor Aharon Oren for helpful advice on the specific epithet.

Funding

This work was supported in part by Grants from the National Key Research and Development Plan (No. 2017YFD0201606), the China Postdoctoral Science Foundation (2018M631907) and the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (UNPYSCT-2017017).

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Authors and Affiliations

  1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Chuanyu Han, Junwei Zhao, Bing Yu, Haoran Shi, Chen Zhang, Xiaowei Guo, Wensheng Xiang & Xiangjing Wang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Wensheng Xiang

Authors
  1. Chuanyu Han
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  2. Junwei Zhao
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  3. Bing Yu
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  5. Chen Zhang
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Contributions

Chuanyu Han performed the laboratory experiments, analyzed the data, and drafted the manuscript. Bing Yu contributed to the biochemical characterization. Junwei Zhao contributed to the polyphasic taxonomy. Haoran Shi contributed to the morphological analyzes. Chen Zhang contributed to the fatty acids determination. Xiaowei Guo participated to the discussions of each section of experiments. Xiangjing Wang and Wensheng Xiang designed the experiments and revised the manuscript.

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Correspondence to Wensheng Xiang or Xiangjing Wang.

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Han, C., Zhao, J., Yu, B. et al. Microbispora tritici sp. nov., a novel actinomycete isolated from a root of wheat (Triticum aestivum L.). Antonie van Leeuwenhoek 112, 1137–1145 (2019). https://doi.org/10.1007/s10482-019-01246-y

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