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Streptomyces ginkgonis sp. nov., an endophyte from Ginkgo biloba

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Abstract

A novel endophytic actinomycete strain, designated KM-1-2T, was isolated from seeds of Ginkgo biloba at Yangling, China. A polyphasic approach was used to study the taxonomy of strain KM-1-2T and it was found to show a range of phylogenetic and chemotaxonomic properties consistent with those of members of the genus Streptomyces. The diamino acid of the cell wall peptidoglycan was identified as LL-diaminopimelic acid. No diagnostic sugars were detected in whole cell hydrolysates. The predominant menaquinones were identified as MK-9(H6) and MK-9(H8). The diagnostic phospholipids were found to be phosphatidylethanolamine and phosphatidylcholine. The DNA G + C content of the novel strain was determined to be 72.9 mol%. The predominant cellular fatty acids (> 10.0 %) were identified as iso-C14 : 0, iso-C16 : 0, C16 : 0 and C17 : 0 cyclo. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain is closely related to Streptomyces carpaticus JCM 6915T (99.3%), Streptomyces harbinensis DSM 42076T (98.9%) and Streptomyces cheonanensis JCM 14549T (98.5%). DNA-DNA hybridizations with these three close relatives gave similarity values of 39.1 ± 1.9, 35.8 ± 2.3, and 47.4 ± 2.7%, respectively, which indicated that strain KM-1-2T represents a novel species of the genus Streptomyces. This is consistent with the morphological, physiological and chemotaxonomic data. Cumulatively, these data suggest that strain KM-1-2T represents a novel Streptomyces species, for which the name Streptomyces ginkgonis sp. nov. is proposed, with the type strain KM-1-2T (= CCTCC AA2016004T = KCTC 39801T).

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Acknowledgements

This work was supported by Foundation Research Project of Shaanxi Province (No. 2017JZ006); National Natural Science Foundation of China (No. 31101476); Science and Technology Program of Yangling Demonstration Zone (No. 2014NY-41).

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, China

    Xia Yan, Yanfang Li, Nana Wang, Yue Chen & Li-li Huang

  2. College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Li-li Huang

  3. College of Life Sciences, Northwest A&F University, Yangling, 712100, China

    Xia Yan, Yanfang Li, Nana Wang & Yue Chen

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  1. Xia Yan
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Correspondence to Li-li Huang.

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Yan, X., Li, Y., Wang, N. et al. Streptomyces ginkgonis sp. nov., an endophyte from Ginkgo biloba . Antonie van Leeuwenhoek 111, 891–896 (2018). https://doi.org/10.1007/s10482-017-0987-3

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