Abstract
Results from recent studies are breaking the paradigm that all viruses depend on their host machinery to glycosylate their proteins. Chloroviruses encode several genes involved in glycan biosynthesis and some of their capsid proteins are decorated with N-linked oligosaccharides with unique features. Here we describe the elucidation of the N-glycan structure of an unusual chlorovirus, NE-JV-1, that belongs to the Pbi group. The host for NE-JV-1 is the zoochlorella Micractinium conductrix. Spectroscopic analyses established that this N-glycan consists of a core region that is conserved in all of the chloroviruses. The one difference is that the residue 3OMe-l-rhamnose is acetylated at the O-2 position in a non-stoichiometric fashion.
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Acknowledgements
Funding for this study was partially provided by the NSF-EPSCoR Grant EPS-1004094 (JVE), the COBRE program of the National Center for Research Resources Grant P20-RR15535 (JVE), and National Institute for General Medical Science Grant 8P20GM103427 (to G.D.).
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Speciale, I., Agarkova, I., Duncan, G.A. et al. Structure of the N-glycans from the chlorovirus NE-JV-1. Antonie van Leeuwenhoek 110, 1391–1399 (2017). https://doi.org/10.1007/s10482-017-0861-3
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DOI: https://doi.org/10.1007/s10482-017-0861-3