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One-step RT-LAMP analysis for efficient detection of Odontoglossum ringspot virus from Oncidiinae hybrids (Orchidaceae)

  • Viral and Viroid Diseases
  • Published:
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Abstract

Odontoglossum ringspot virus (ORSV) is one of the most serious pathogens in the orchid industry. The production of virus-free orchid seedlings is the most efficient strategy to counter this virus problem. Therefore, an easy, efficient process to detect viruses is important to the orchid industry. One-step reverse transcription (RT) loop-mediated isothermal amplification (LAMP) technique was developed to detect ORSV in Oncidiinae hybrids. RT-LAMP analysis primers were designed based on the conserved regions of the RNA-dependent RNA polymerase (RdRp) gene among ORSV isolates. RT-LAMP reaction conditions were optimized to detect ORSV from Oncidiinae hybrids. This one-step RT-LAMP method proved to be easy, efficient, sensitive, and inexpensive for detecting ORSV in orchids and is the first application of a one-step RT-LAMP to detect ORSV.

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Acknowledgments

This study is dedicated to the memory of Dr. C.C. Tsai, who unexpectedly passed away on November 05, 2015. Dr. C.C. Tsai was a research scientist studying genetics and breeding at the Crops Improvement Division, Kaohsiung District Agricultural Research and Extension Station, Pingtung, Taiwan. This research was funded by the Ministry of Science and Technology, Taiwan, MOST 103-2621-B-067E-001-MY3 to Chi-Chu Tsai.

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Authors and Affiliations

  1. Kaohsiung District Agricultural Research and Extension Station, Pingtung, 908, Taiwan

    Chi-Chu Tsai, Chun-Yen Huang, I-Szu Weng & Wen-Lin Liu

  2. National Pingtung University of Science and Technology, Pingtung, 912, Taiwan

    Chi-Chu Tsai, Chun-Yen Huang & I-Szu Weng

  3. Research Center for Biodiversity, China Medical University, Taichung, 404, Taiwan

    Chun-Yen Huang, I-Szu Weng & Chang-Hung Chou

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  1. Chi-Chu Tsai
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  2. Chun-Yen Huang
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  3. I-Szu Weng
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  4. Wen-Lin Liu
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  5. Chang-Hung Chou
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Correspondence to Chi-Chu Tsai.

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Tsai, CC., Huang, CY., Weng, IS. et al. One-step RT-LAMP analysis for efficient detection of Odontoglossum ringspot virus from Oncidiinae hybrids (Orchidaceae). J Gen Plant Pathol 82, 89–95 (2016). https://doi.org/10.1007/s10327-016-0645-z

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  • DOI: https://doi.org/10.1007/s10327-016-0645-z

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