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Curcumin inhibits the expression of proinflammatory mediators and MMP-9 in gingival epithelial cells stimulated for a prolonged period with lipopolysaccharides derived from Porphyromonas gingivalis

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Abstract

Curcumin, a yellow phytochemical found in the rhizomes of Curcuma longa, has various biological effects, including anti-oxidant and anti-inflammatory activities. In the present study, we examined the effect of curcumin on the expression of inflammatory cytokines in human gingival epithelial progenitor cells (HGEPs) stimulated for a prolonged period with lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. The cells were alternately cultured with LPS and/or curcumin every 3 days for 18 days. The expression levels of TNF-α, IL-1β, IL-6, TIMP-1, and MMP-9 in the HGEPs were evaluated by quantitative real-time polymerase chain reaction. Enzyme-linked immunosorbent assay was used to measure the concentrations of these five proteins in the supernatant and nuclear factor (NF)-κB in the nuclear extracts. Curcumin inhibited the mRNA expression levels of TNF-α, IL-1β, IL-6, and MMP-9 in HGEPs treated with curcumin over a prolonged period. Similarly, the expression levels of IL-1β, IL-6, and MMP-9 were decreased in the culture supernatants. NF-κB activity was also inhibited in the cells cultured with curcumin. In conclusion, these findings indicate that curcumin inhibits the expression of inflammatory cytokines and MMP-9 in primary gingival epithelial cells stimulated with P. gingivalis-derived LPS via NF-κB activation.

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Acknowledgements

This study was supported in part by a Grant-in-Aid for the 2017–2018 Research Project of the Research Institute of Personalized Health Sciences, Health Sciences University of Hokkaido.

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Authors and Affiliations

  1. Division of Disease Control and Molecular Epidemiology, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan

    Seiko Toraya, Osamu Uehara & Itsuo Chiba

  2. Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan

    Daichi Hiraki, Puja Neopane, Tetsuro Morikawa, Koki Yoshida, Hirofumi Matsuoka & Yoshihiro Abiko

  3. Division of Oral and Maxillofacial Surgery, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan

    Fumiya Harada

  4. The Research Institute of Health Sciences, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan

    Rie Takai

  5. Department of Ophthalmology, Health Sciences University of Hokkaido Hospital, 2-5 Ainosato, Kita-ku, Sapporo, Hokkaido, 002-8072, Japan

    Nobuyoshi Kitaichi

Authors
  1. Seiko Toraya
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  2. Osamu Uehara
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  3. Daichi Hiraki
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  9. Hirofumi Matsuoka
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  10. Nobuyoshi Kitaichi
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  11. Itsuo Chiba
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  12. Yoshihiro Abiko
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Correspondence to Yoshihiro Abiko.

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Toraya, S., Uehara, O., Hiraki, D. et al. Curcumin inhibits the expression of proinflammatory mediators and MMP-9 in gingival epithelial cells stimulated for a prolonged period with lipopolysaccharides derived from Porphyromonas gingivalis. Odontology 108, 16–24 (2020). https://doi.org/10.1007/s10266-019-00432-8

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  • DOI: https://doi.org/10.1007/s10266-019-00432-8

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