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Application of cationized magnetoferritin for magnetic field-assisted delivery of short interfering RNA in vitro

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Abstract

Cationized magnetoferritin is used for development of a simple, efficient, and fast delivery of short interference RNA into cells using combination of magnetophoresis for pre-concentration of siRNA-magnetoferritin complex on the surface of plated cells with subsequent application of nanosecond laser pulses producing stress waves in transfection chamber, which permeabilize cell membrane for the facilitated delivery of siRNA into the cell interior. As has been quantified using siRNA inducing cell death assay, by combination of these two physical factors we have obtained high efficiency for tested three different human carcinoma cells. Proposed method of gene silencing based on cationized magnetoferritin is a versatile and easily accessible platform with many possible applications in gene therapy.

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Funding

This work was supported by a grant of Slovak grant agencies VEGA 1/0810/18 and APVV 16-0600.

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Authors and Affiliations

  1. Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina F1, 842 48, Bratislava, Slovakia

    Melánia Babincová & Peter Babinec

  2. Department of Surgical Oncology, Saint Elisabeth Cancer Institute and Faculty of Medicine, Comenius University Bratislava, Heydukova 10, Bratislava, Slovakia

    Štefan Durdík

  3. Department of Dermatovenerology, Faculty of Medicine, Mickiewiczova 13, 813 69, Bratislava, Slovakia

    Natália Babincová

  4. Oklahoma Cancer Specialists and Research Institute, 12697 East 51st Street South, Tulsa, OK, 74146, USA

    Paul Sourivong

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  1. Melánia Babincová
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  2. Štefan Durdík
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  5. Peter Babinec
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Correspondence to Peter Babinec.

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Babincová, M., Durdík, Š., Babincová, N. et al. Application of cationized magnetoferritin for magnetic field-assisted delivery of short interfering RNA in vitro. Lasers Med Sci 33, 1807–1812 (2018). https://doi.org/10.1007/s10103-018-2547-0

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  • DOI: https://doi.org/10.1007/s10103-018-2547-0

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