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Diversity analysis of Saccharomyces cerevisiae isolated from natural sources by multilocus sequence typing (MLST)

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Abstract

We used multilocus sequence typing (MLST) to analyze the diversity of natural isolates of Saccharomyces cerevisiae, the most important microorganism in alcoholic fermentation. Six loci, ADP1, RPN2, GLN4, ACC1, MET4, and NUP116, in S. cerevisiae genome were selected as MLST markers. To investigate genetic diversity within S. cerevisiae, 42 S. cerevisiae isolated from natural sources in Korea as well as six S. cerevisiae obtained from Genbank and four industrial S. cerevisiae were examined using MLST. Twenty-six polymorphic sites were found in the six loci. Among them, ACC1 had the most genetic variation with eight polymorphic sites. MLST differentiated the 52 strains into three clades. Alcohol fermentation results revealed that S. cerevisiae in Clade III produced less alcohol than those in Clades I and II. These results suggested that MLST is a powerful tool to differentiate S. cerevisiae and can potentially be used to select S. cerevisiae suitable for industrial use.

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Acknowledgements

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201629202).

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Authors and Affiliations

  1. Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104, Korea

    You-Jung Eeom, Su-Yeong Son, Dong-Hyun Jung & Cheon-Seok Park

  2. Microorganism Resources Division, National Institute of Biological Resources, Incheon, 22689, Korea

    Moon-Suk Hur & Chang-Mu Kim

  3. Research Institute, Kooksoondang Brewery Co. Ltd., Seongnam, 13202, Korea

    Sun-Young Park & Woo-Chang Shin

  4. College of Pharmacy, Sookmyung Women’s University, Seoul, 04310, Korea

    Sang-Jin Lee

  5. Department of Food Science and Technology, Chung-Ang University, Ansung, 17546, Korea

    Joong-Hyuck Auh

  6. Brewing Research Center, Academic Industry Cooperation, Hankyong National University, Anseong, 17579, Korea

    Gye-Won Kim

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  1. You-Jung Eeom
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  2. Su-Yeong Son
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Correspondence to Cheon-Seok Park.

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Eeom, YJ., Son, SY., Jung, DH. et al. Diversity analysis of Saccharomyces cerevisiae isolated from natural sources by multilocus sequence typing (MLST). Food Sci Biotechnol 27, 1119–1127 (2018). https://doi.org/10.1007/s10068-018-0335-z

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  • DOI: https://doi.org/10.1007/s10068-018-0335-z

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