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Rapd analysis of wine Saccharomyces cerevisiae strains differentiated by pulsed field gel electrophoresis

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Biotechnology Techniques

Summary

Enological yeast strains involved simultaneously during a fermentation can be identified through the analysis of their electrophoretic karyotype. The right assignment of yeasts to different strains has been checked by analysing the random amplified polymorphic DNA (RAPD), using a simple minipreparation protocol to obtain the template DNA for the polymerase chain reaction (PCR).

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Authors and Affiliations

  1. Laboratorio di genetica molecolare, Istituto Agrario di San Michele all'Adige, Via Mach 1, 38010, San Michele all'Adige, Trente, Italy

    M. S. Grando

  2. Departamento de Química Analítica y Tecnología de alimentos, Universidad de Castilla - La Mancha, Campus Universitario, 13072, Ciudad Real, España

    J. Ubeda & A. I. Briones

Authors
  1. M. S. Grando
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  2. J. Ubeda
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  3. A. I. Briones
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Grando, M.S., Ubeda, J. & Briones, A.I. Rapd analysis of wine Saccharomyces cerevisiae strains differentiated by pulsed field gel electrophoresis. Biotechnol Tech 8, 557–560 (1994). https://doi.org/10.1007/BF00152145

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  • DOI: https://doi.org/10.1007/BF00152145

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