Abstract
In vitro and in vivo antihypertensive effects of grass carp peptides (GCP) were investigated. The amino acid composition and the angiotensinconverting enzyme inhibitory activity were evaluated using HPLC. GCP was administrated to spontaneously hypertensive rats (SHRs) and Wistar Kyoto rats (WKY) by single and long-term administration before blood pressure measurements. Plasma levels of angiotensin II (Ang II), rennin (RA), nitric oxide (NO), and plasma angiotensin I converting enzyme (ACE) activity were measured. The GCP molecular weight was between 725 and 1,228 Da with high levels of Leu, Asp, Phe, Gly, and Pro. The in vitro IC50 value was 0.23 mg/mL. GCP lowered SHR blood pressure dose and timedependently. Little change occurred in WKY. The plasma level of Ang II and the ACE activity decreased. SHR RA and NO concentrations in plasma increased. The antihypertensive effect of GCP was associated with NO regulation and the rennin-angiotensin system.
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Androulakis ES, Tousoulis D, Papageorgiou N, Tsioufis C, Kallikazaros I, Stefanadis C. Essential hypertension: Is there a role for inflammatory mechanisms. Cardiol. Rev. 17: 216–221 (2009)
Chockalingam A, Campbell NR, Fodor JG. Worldwide epidemic of hypertension. Can. J. Cardiol. 22: 553–555 (2006)
Jang JH, Jeong SC, Kim JH, Lee YH, Ju YC, Lee JS. Characterisation of a new antihypertensive angiotensin I-converting enzyme inhibitory peptide from Pleurotus cornucopiae. Food Chem. 127: 412–418 (2011)
Oshima G, Shimabukuro H, Nagasawa K. Peptide inhibitors of angiotensin I-converting enzyme in digests of gelatin by bacterial collagenase. Biochim. Biophys. Acta 566: 128–137 (1979)
Pihlanto-Leppala A, Koskinen P, Piilola K, Tupasela T, Korhonen H. Angiotensin I-converting enzyme inhibitory properties of whey protein digests: Concentration and characterization of active peptides. J. Dairy. Res. 67: 53–64 (2000)
Wang X, Wang L, Cheng X, Zhou J, Tang X, Mao XY. Hypertension-attenuating effect of whey protein hydrolysate on spontaneously hypertensive rats. Food Chem. 134: 122–126 (2012)
Arihara K, Nakashima Y, Mukai T, Ishikawa S, Itoh M. Peptide inhibitors for angiotensin I-converting enzyme from enzymatic hydrolysates of porcine skeletal muscle proteins. Meat Sci. 57: 319–324 (2001)
Fritz M, Vecchi B, Rinaldi G, Añón MC. Amaranth seed protein hydrolysates have in vivo and in vitro antihypertensive activity. Food Chem. 126: 878–884 (2011)
Ishiguro K, Sameshima Y, Kume T, Ikeda K, Matsumoto J, Yoshimoto M. Hypotensive effect of a sweetpotato protein digest in spontaneously hypertensive rats and purification of angiotensin Iconverting enzyme inhibitory peptides. Food Chem. 131: 774–779 (2012)
Zhao YH, Li BF, Dong SY, Liu ZY, Zhao X, Wang JF, Zeng MY. A novel ACE inhibitory peptide isolated from Acaudina molpadioidea hydrolysate. Peptides 30: 1028–1033 (2009)
Fujita H, Yoshikawa M. LKPNM: A prodrug-type ACE-inhibitory peptide derived from fish protein. Immunopharmacology 44: 123–127 (1999)
Suetsuna K. Antioxidant peptides from the protease digest of prawn (Penaeus japonicus) muscle. Mar. Biotechnol. 2: 5–10 (2000)
Li L, Zong L, Wang JJ, Cheng SJ. Research status and development trend of massive freshwater fish pre-treatment processing technology and equipment. Fish. Modernization 5: 43–47 (2010)
Chen JW, Wang YM, Zhong QX, Wu YN, Xia WS. Purification and characterization of a novel angiotensin-I converting enzyme (ACE) inhibitory peptide derived from enzymatic hydrolysate of grass carp protein. Peptides 33: 52–58 (2012)
Wang YM. Isolation, Purification and quantitative structure activity relationship of ACE inhibitory activity peptides from grass carp protein. MS thesis, Wuhan Polytechnic University, Wuhan, China (2011)
Podzimek S, The use of GPC coupled with a multiangle laser light scattering photometer for the characterization of polymers. On the determination of molecular weight, size and branching. J. Appl. Polym. Sci. 54: 91–103 (1994)
Adeyeye EI. Amino acid composition of three species of Nigerian sh: Clarias anguillaris, Oreochromis niloticus, and Cynoglossus senegalensis. Food Chem. 113: 43–46 (2009)
Wu JP, Aluko RE, Muir AD. Improved method for direct highperformance liquid chromatography assay of angiotensin-converting enzyme-catalyzed reactions. J. Chromatogr. A 950: 125–130 (2002)
Luo LF, Wu WH, Zhou YJ, Yan J, Yang GP, Ouyang DS. Antihypertensive effect of Eucommia ulmoides Oliv. extracts in spontaneously hypertensive rats. J. Ethnopharmacol. 129: 238–243 (2010)
Liu X, Zhang MS, Zhang C, Liu CH. Angiotensin converting enzyme (ACE) inhibitory, antihypertensive and antihyperlipidaemic activities of protein hydrolysates from Rhopilema esculentum. Food Chem. 134: 2134–2140 (2012)
Hernández-Ledesma B, Mar Contreras MD, Recio I. Antihypertensive peptides: Production, bioavailability, and incorporation into foods. Adv. Colloid. Interfac. 165: 23–35 (2011)
Li GH, Le GW, Shi YH, Shrestha S. Angiotensin I-converting enzyme inhibitory peptides derived from food proteins and their physiological and pharmacological effects. Nutr. Res. 24: 469–486 (2004)
Elizabeth RC, William JD. Amino acid side chain descriptors for quantitative structure-activity relationship studies of peptide analogs. J. Med. Chem. 38: 2705–2713 (1995)
Nakamura Y, Yamamoto N, Sakai K, Okubo A, Yamazaki S, Takano T. Purification and characterization of angiotensin Iconverting enzyme inhibitors from sour milk. J. Dairy Sci. 78: 777–783 (1995)
Bougatef A, Nedjar-Arroume N, Ravallec-Plé R, Leroy Y, Guillochon D, Barkia A, Nasri M. Angiotensin I-converting enzyme (ACE) inhibitory activities of sardinelle (Sardinella aurita) byproducts protein hydrolysates obtained by treatment with microbial and visceral fish serine proteases. Food Chem. 111: 350–356 (2008)
Peach MJ. Renin-angiotensin system: Biochemistry and mechanisms of action. Physiol. Rev. 57: 313–370 (1977)
Staljanssens D, Camp VJ, Herregods G, Dhaenens M, Deforce D, De Voorde JV, Smagghe G. Antihypertensive effect of insect cells: In vitro and in vivo evaluation. Peptides 32: 526–530 (2011)
Ngo DH, Vo TS, Ngo DN, Wijesekara I, Kim SK. Biological activities and potential health benefits of bioactive peptides derived from marine organisms. Int. J. Biol. Macromol. 51: 378–383 (2012)
Friebe A, Koesling D. Regulation of nitric oxide-sensitive guanylyl cyclase. Circ. Res. 93: 96–105 (2003)
Looft-Wilson RC, Billaud M, Johnstone SR, Straub AC, Isakson BE. Interaction between nitric oxide signaling and gap junctions: Effects on vascular function. BBA-Biomembranes 1818: 1895–1902 (2012)
Desjardins F, Balligand J. Nitric oxide-dependent endothelial function and cardiovascular disease. Acta Clin. Belg. 61: 326–334 (2006)
Zhao CX, Xu XZ, Cui YH, Wang PH, Wei X, Yang SL, Edin ML, Zeldin DC, Wang DW. Increased endothelial nitric-oxide synthase expression reduces hypertension and hyperinsulinemia in fructosetreated rats. J. Pharmacol. Exp. Ther. 328: 610–620 (2009)
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Wang, S., Lin, Lm., Wu, Yn. et al. Angiotensin I Converting Enzyme (ACE) inhibitory activity and antihypertensive effects of grass carp peptides. Food Sci Biotechnol 23, 1661–1666 (2014). https://doi.org/10.1007/s10068-014-0226-x
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DOI: https://doi.org/10.1007/s10068-014-0226-x