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Effects of Catechin on Activity of Angiotensin-Converting Enzyme and Generation of Reactive Oxygen Species in Rat Aorta

  • BIOPHYSICS AND BIOCHEMISTRY
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Activity of angiotensin-converting enzyme (ACE) and intensity of ROS generation in the aorta were studied in male Wistar rats intraperitoneally injected with a mixture of (+) and (-) stereoisomers of catechin. ACE activity in aortal segments was evaluated by hydrolysis of hippuryl-histidine-leucine; ROS generation was measured by oxidation of dichlorodihydrofluorescein. The dynamics of ACE activity and intensity of ROS generation in the aorta after catechin administration and their dependence on the catechin dose were studied. The effects of dihydroquercetin and fucoidin on the studied parameters were analyzed. Catechin increased ACE activity; the maximum increase was achieved in 3 h after administration. Catechin dose producing a half-maximum increase in ACE activity was 0.04 μg/kg. The effects of catechin on ACE activity was attenuated by dihydroquercetin and completely abolished by fucoidin. Catechin did not enhance ROS production in the aorta, and in a dose of 0.1 μg/kg even inhibited this process. It is hypothesized that isomers of catechin produce opposite effects on ROS generation in the aorta.

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References

  1. Ackermann A, Fernández-Alfonso MS, Sánchez de Rojas R, Ortega T, Paul M, González C. Modulation of angiotensin-converting enzyme by nitric oxide. Br. J. Pharmacol. 1998;124(2):291-298.

    CAS  Google Scholar 

  2. Almeida Rezende B, Pereira AC, Cortes SF, Lemos VS. Vascular effects of flavonoids. Curr. Med. Chem. 2016;23(1):87-102.

    Article  CAS  Google Scholar 

  3. Dzau VJ. Theodore Cooper Lecture: Tissue angiotensin and pathobiology of vascular disease: a unifying hypothesis. Hypertension 2001;37(4):1047-1052.

    Article  CAS  Google Scholar 

  4. Grassi D, Desideri G, Ferri C. Flavonoids: antioxidants against atherosclerosis. Nutrients. 2010;2(8):889-902.

    Article  CAS  Google Scholar 

  5. Griendling KK, Minieri CA, Ollerenshaw JD, Alexander RW. Angiotensin II stimulates NADH and NADPH oxidase activity in cultured vascular smooth muscle cells. Circ. Res. 1994;74(6):1141-1148.

    Article  CAS  Google Scholar 

  6. Heeneman S, Sluimer JC, Daemen MJ. Angiotensin-converting enzyme and vascular remodeling. Circ. Res. 2007;101(5):441-454.

    Article  CAS  Google Scholar 

  7. Hodgson JM, Croft KD. Tea flavonoids and cardiovascular health. Mol. Aspects Med. 2010;31(6):495-502.

    Article  CAS  Google Scholar 

  8. Ikeda I, Tsuda K, Suzuki Y, Kobayashi M, Unno T, Tomoyori H, Goto H, Kawata Y, Imaizumi K, Nozawa A, Kakuda T. Tea catechins with a galloyl moiety suppress postprandial hypertriacylglycerolemia by delaying lymphatic transport of dietary fat in rats. J. Nutr. 2005;135(2):155-159.

    Article  CAS  Google Scholar 

  9. Kanwar S, Bullard DC, Hickey MJ, Smith CW, Beaudet AL, Wolitzky BA, Kubes P. The association between alpha4-integrin, P-selectin, and E-selectin in an allergic model of inflammation. J. Exp. Med. 1997;185(6):1077-1087.

    Article  CAS  Google Scholar 

  10. Kofink M, Papagiannopoulos M, Galensa R. (-)-Catechin in cocoa and chocolate: occurrence and analysis of an atypical flavan-3-ol enantiomer. Molecules 2007;12(7):1274-1288.

    Article  CAS  Google Scholar 

  11. Korystov YN, Emel’yanov MO, Korystova AF, Levitman MK, Shaposhnikova VV. Determination of reactive oxygen and nitrogen species in rat aortausing the dichlorofluorescein assay. Free Radic. Res. 2009;43(2):149-155.

    Article  CAS  Google Scholar 

  12. Korystova AF, Kublik LN, Kim YA, Levitman MK, Shaposhnikova VV, Korystov YN. Dihydroquercetin and fucoidin inhibit the increase of angiotensin-converting enzyme activity in the rat aorta after irradiation. Bull. Exp. Biol. Med. 2018;165(3):360-363.

    Article  CAS  Google Scholar 

  13. Nyfeler F, Moser UK, Walter P. Stereospecific effects of (+)- and (−)-catechin on glycogen metabolism in isolated rat hepatocytes. Biochim. Biophys. Acta 1983;763(1):50-57.

    Article  CAS  Google Scholar 

  14. Smith SW. Chiral toxicology: it’s the same thing...only different. Toxicol. Sci. 2009;110(1):4-30.

  15. van het Hof KH, Kivits GA, Weststrate JA, Tijburg LB. Bioavailability of catechins from tea: the effect of milk. Eur. J. Clin. Nutr. 1998;52(5):356-359.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Moscow region, Russia

    V. A. Anikina, A. F. Korystova, M. Kh. Levitman, V. V. Shaposhnikova & Yu. N. Korystov

  2. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia

    Yu. A. Kim

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  1. V. A. Anikina
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  2. Yu. A. Kim
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  3. A. F. Korystova
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  4. M. Kh. Levitman
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  5. V. V. Shaposhnikova
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  6. Yu. N. Korystov
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Correspondence to Yu. N. Korystov.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 11, pp. 565-568, November, 2019

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Anikina, V.A., Kim, Y.A., Korystova, A.F. et al. Effects of Catechin on Activity of Angiotensin-Converting Enzyme and Generation of Reactive Oxygen Species in Rat Aorta. Bull Exp Biol Med 168, 627–630 (2020). https://doi.org/10.1007/s10517-020-04766-0

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  • DOI: https://doi.org/10.1007/s10517-020-04766-0

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