Skip to main content
SpringerLink
Log in

Identification of QTLs for seed germination capability after various storage periods using two RIL populations in rice

  • Published:
Molecules and Cells

Abstract

Seed germination capability of rice is one of the important traits in the production and storage of seeds. Quantitative trait loci (QTL) associated with seed germination capability in various storage periods was identified using two sets of recombinant inbred lines (RILs) which derived from crosses between Milyang 23 and Tong 88-7 (MT-RILs) and between Dasanbyeo and TR22183 (DT-RILs). A total of five and three main additive effects (QTLs) associated with seed germination capability were identified in MT-RILs and DT-RILs, respectively. Among them, six QTLs were identified repeatedly in various seed storage periods designated as qMT-SGC5.1, qMT-SGC7.2, and qMT-SGC9.1 on chromosomes 5, 7, and 9 in MT-RILs, and qDT-SGC2.1, qDT-SGC3.1, and qDT-SGC9.1 on chromosomes 2, 3, and 9 in DT-RILs, respectively. The QTL on chromosome 9 was identified in both RIL populations under all three storage periods, explaining up to 40% of the phenotypic variation. Eight and eighteen pairs additive × additive epistatic effect (epistatic QTL) were identified in MT-RILs and DT-RILs, respectively. In addition, several near isogenic lines (NILs) were developed to confirm six repeatable QTL effects using controlled deterioration test (CDT). The identified QTLs will be further studied to elucidate the mechanisms controlling seed germination capability, which have important implications for long-term seed storage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bentsink, L., Alonso-Blanco, C., Vreugdenhil, D., Tesnier, K., Groot, S.P.C., and Koornneef, M. (2000). Genetic analysis of seedsoluble oligosaccharides in relation to seed storability of Arabidopsis. Plant Physiol. 124, 1595–1604.

    Article  PubMed  CAS  Google Scholar 

  • Bewley, J.D., and Black, M. (1994). Seeds: physiology of development and germination. (New York, NY, USA: Plenum Press).

    Google Scholar 

  • Cho, Y.I., Jiang, W., Chin, J.H., Piao, Z., Cho, Y.G., McCouch, S.R., and Koh, H.J., (2007). Identification of QTLs associated with physiological nitrogen use efficiency in rice. Mol. Cells 23, 72–79.

    PubMed  CAS  Google Scholar 

  • Chung, G.S., and Heu, M.H. (1991). Improvement of tongil-type rice cultivars from indica/japonica hybridization in Korea. In Biotechnology in Agriculture and Forestry 14-Rice, Y.P.S. Bajaj, ed. (Springer, Berlin Heidelberg, New York), pp. 105–112.

    Google Scholar 

  • Clerkx, E.J.M., Blankestijn-De Vries, H., Ruys, G.J., Groot, S.P.C., and Koornneef, M. (2004). Genetic differences in seed longevity of various Arabidopsis mutants. Physiol. Plant 121, 448–461.

    Article  CAS  Google Scholar 

  • Doi, K., Yasui, H., and Yoshimura, A. (2008). Genetic variation in rice. Curr. Opin. Plant Biol. 11, 144–148.

    Article  PubMed  CAS  Google Scholar 

  • Delouche, J.C., and Baskin, C.C. (1973). Accelerated aging techniques for predicting the relative storability of seed lots. Seed Sci. Technol. 1, 427–452.

    Google Scholar 

  • Ellis, R.H., Roberts, and E.H. (1981). The quantification of aging and survival in orthodox seeds. Seed Sci. Technol. 9, 373–409.

    Google Scholar 

  • Ellis, R.H., Osei-Bonsu, K., and Roberts, E.H. (1982). The influence of genotype, temperature and moisture on seed longevity in chickpea, cowpea and soybean. Ann. Bot. 50, 69–82.

    Google Scholar 

  • Falconer, D.S., and Mackay, T.F.C. (1996). Introduction to quantitative genetics. (Harlow, Essex, UK: Longmans Green).

    Google Scholar 

  • Gu, X.Y., Kianian, S.F., and Foley, M.E. (2004). Multiple loci and epistases control genetic variation for seed dormancy in weedy rice (Oryza sativa). Genetics 166, 1503–1516.

    Article  PubMed  CAS  Google Scholar 

  • Hill, J., Becker, H.C., and Tigerstedt, P.M.A. (1998). Quantitative and ecological aspects of plant breeding. (London, UK: Chapman & Hall).

    Google Scholar 

  • Jiang, W., Chu, S.H., Piao, R.H., Chin, J.H., Jin, Y.M., Lee, J., Qiao, Y., Han, L., Piao, Z., and Koh, H.J. (2008). Fine mapping and candidate gene analysis of hwh1 and hwh2, a set of complementary genes controlling hybrid breakdown in rice. Theor. Appl. Genet. 116, 1117–1127.

    Article  PubMed  CAS  Google Scholar 

  • Jiang, W., Lee, J., Chu, S.H., Ham, T.H., Woo, M.O., Cho, Y.I., Chin, J.H., Han, L.Z., Xuan, Y., Yuan, D., et al. (2010). Genotype × environment interactions for chilling tolerance of rice recombinant inbred lines under different low temperature environments. Field Crops Res. 117, 226–236.

    Article  Google Scholar 

  • Kameswara, N., and Jackson, M.T. (1996). Seed longevity of environment and storage longevity of japonica rices (Oryza sativa L.). Seed Sci. Res. 6, 17–21.

    Google Scholar 

  • Kameswara, N., and Jackson, M.T. (1997). Variation in seed longevity of rice cultivars belonging to different isozyme groups. Gene Res. Crop Evol. 44, 159–164.

    Article  Google Scholar 

  • Kim, K.M., Kown, Y.S., Lee, J.J., Eun, M.Y., and Shon, J.K. (2004). QTL mapping and molecular marker analysis for the resistance of rice to ozone. Mol. Cells 17, 151–155.

    PubMed  Google Scholar 

  • Kosambi, D.D. (1944). The estimation of map distances from recombination values. Ann. Eugn. 12, 172–175.

    Article  Google Scholar 

  • Kwon, Y.S., Kim, K.M., Eun, M.Y., and Sohn, J.K. (2001). Quantitative trait loci mapping associated with plant regeneration ability from seed derived calli in rice (Oryza sativa L.). Mol. Cells 11, 64–67.

    PubMed  CAS  Google Scholar 

  • Lincoln, S., Daly, M., and Lander, E.S. (1992). Constructing genetic maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report, 2nd eds. (Massachusetts, USA: Whitehead Institute, Cambridge).

    Google Scholar 

  • McCouch, S.R., Cho, Y.G., Yano, M., Paul, E., and Kinoshita, T. (1997). Report on QTL nomenclature. Rice Genet. Newslett. 14, 11–13.

    Google Scholar 

  • Miura, K., Lin, S.Y., Yano, M., and Nagamine, T. (2002). Mapping quantitative trait loci controlling seed longevity in rice (Oryza sativa L.). Theor. Appl. Genet. 104, 981–986.

    Article  PubMed  CAS  Google Scholar 

  • Nelson, J.C. (1997). QGENE, software for marker-based genome analysis and breeding. Mol. Breed. 3, 239–245.

    Article  CAS  Google Scholar 

  • Padma, V., and Reddy, B.M. (2000). Evaluation of rice genotypes for dormancy duration and seed storability under natural and accelerated ageing. Seed Res. 28, 158–165.

    Google Scholar 

  • Powell, A., and Matthews, S. (1984). Application of the controlled deterioration vigour test to detect seed lots of Brussels sprouts with low potential for storage under commercial conditions. Seed Sci. Technol. 12, 649–657.

    Google Scholar 

  • Rahman, M.L., Jiang, W., Chu, S.H., Qiao, Y., Ham, T.H., Woo, M.O., Lee, J.H., Khanam, M.S., Chin, J.H., Jeung, J.U., et al. (2009). High-resolution mapping of two rice brown planthopper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minuta. Theor. Appl. Genet. 119, 1237–1246.

    Article  PubMed  Google Scholar 

  • Rajjou, L., and Debeaujon, I. (2008). Seed longevity: survival and maintenance of high germination ability of dry seeds. C.R. Biol. 331, 796–805.

    Article  PubMed  Google Scholar 

  • Roberts, E.H. (1972). Loss of viability and crop yields. In Viability of Seeds, E.H. Roberts, ed. (London, UK: Chapman and Hall), pp. 307–359.

    Google Scholar 

  • SAS Institute Inc. (1999). SAS/Stat User’s Guide, Version 8.2. SAS institute, Inc., Cary, NC, USA.

    Google Scholar 

  • Sasaki, K., Fukuta, Y., and Sato, T. (2005). Mapping of quantitative trait loci controlling seed longevity of rice (Oryza sativa L.) after various periods of seed storage. Plant Breed. 124, 361–366.

    Article  Google Scholar 

  • Shigemune, A., Miura, K., Sasahara, H., Goto, A., and Yoshida, T. (2008). Role of maternal tissues in qLG-9 control of seed longevity in rice (Oryza sativa L.). Breed. Sci. 58, 1–5.

    Article  Google Scholar 

  • Siddique, S.B., Seshu, D.V., and Pardee, W.D. (1988). Rice cultivar variability in tolerance for accelerated aging of seed. IRRI Res. Pap. Ser. 131, 2–7.

    Google Scholar 

  • Singh, R.K., and Ram, H.H. (1986). Inheritance study of soybean seed storability using an accelerated aging test. Field Crops Res. 13, 89–98.

    Article  Google Scholar 

  • Wang, D.L., Zhu, J., Li, Z.K., and Paterson, A.H. (1999). Mapping QTLs with epistatic effects and QTL × environment interactions by mixed linear model approaches. Theor. Appl. Genet. 99, 1255–1264.

    Article  Google Scholar 

  • Xue, Y., Zhang, S.Q., Yao, Q.H., Peng, R.H., Xiong, A.S., Li, X., Zhu, W.M., Zhu, Y.Y., and Zha, D.S. (2008). Identification of quantitative trait loci for seed storability in rice. Euphytica 164, 739–744.

    Article  CAS  Google Scholar 

  • Yamamoto, T., Yonemaru, J., and Yano, M. (2009). Towards the understanding of complex traits in rice: substantially of superficially? DNA Res. 16, 141–154.

    Article  PubMed  CAS  Google Scholar 

  • Yang, X., Guo, Y., Yan, J., Zhang, J., Song, T., Rocheford, T., and Li, J.S. (2010). Major and minor QTL and epistasis contribute to fatty acid composition and oil content in high-oil maize. Theor. Appl. Genet. 120, 665–678.

    Article  PubMed  CAS  Google Scholar 

  • Yano, M., Kojima, S., Takahashi, Y., Lin, H.X., and Sasaki, T. (2001). Genetic control of flowering time in rice, a short-day plant. Plant Physiol. 127, 1425–1429.

    Article  PubMed  CAS  Google Scholar 

  • Zeng, D.L., Qian, Q., Yasukumi, K., Teng, S., and Hirosi, F. (2002). Study on storability and morphological index in rice (Oryza sativa L.) under artificial aging. Acta Agron. Sin. 28, 551–554.

    Google Scholar 

  • Zeng, D.L., Guo, L.B., Xu, Y.B., Yasukumi, K., Zhu, L.H., and Qian, Q. (2006). QTL analysis of seed storability in rice. Plant Breed. 125, 57–60.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Plant Science, Jilin University, Changchun, 130062, China

    Wenzhu Jiang, Hong-Yu Pan & Xinglin Du

  2. Department of Plant Science, Research Institute of Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul, 151-921, Korea

    Joohyun Lee, Yongli Qiao, Rihua Piao, Sun Mi Jang, Mi-Ok Woo & Hee-Jong Koh

  3. School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul, 151-921, Korea

    Yong-Mei Jin

  4. Department of Agricultural Sciences, Korea National Open University, Seoul, 110-791, Korea

    Soon-Wook Kwon

  5. Department of Agricultural Sciences, Yanbian University, Yanji, 133000, China

    Xianhu Liu

Authors
  1. Wenzhu Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joohyun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yong-Mei Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yongli Qiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rihua Piao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sun Mi Jang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mi-Ok Woo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Soon-Wook Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Xianhu Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Hong-Yu Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Xinglin Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Hee-Jong Koh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xinglin Du or Hee-Jong Koh.

About this article

Cite this article

Jiang, W., Lee, J., Jin, YM. et al. Identification of QTLs for seed germination capability after various storage periods using two RIL populations in rice. Mol Cells 31, 385–392 (2011). https://doi.org/10.1007/s10059-011-0049-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10059-011-0049-z

Keywords

Search

Navigation