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Comparative phenotypic and physiological characteristics of spotted Leaf 6 (spl6) and brown leaf Spot2 (bl2) Lesion Mimic Mutants (LMM) in rice

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Molecules and Cells

Abstract

Spontaneous necrotic lesions were found in a lesion mimic mutant brown leaf spot 2 (bl2) without pathogenic infection. Small spots in the seedlings appeared at the four leaves stage and gradually grew into a large round and black area with a gray center on the leaf surfaces. Lower growth habit and lower agronomic trait values with reduced stature, tiller, and panicle number, as well as lower yield potential were noted in the mutants relative to the trait values of the wild-type plants. Microscopic analysis revealed that mesophyll chloroplast was severely damaged or absent in the spotted area of the mutant leaves. Total chlorophyll content, hydrogen peroxide level, and catalase activity were increased at up to 45 days after germination and were dropped at 60 d in the mutant leaves. However, the total protein contents were reduced slightly with a growth period of up to 45 days and were increased at 60 days after germination. A gradual increment of the total ascorbic acid contents in the mutants were observed with advanced plant age, but increased until 45 days and dropped comparatively at 60 days in the wild-type leaves. Increased gene transcriptions of OsPDI and OsGPX1 were noted in the spotted leaves as compared to the non-spotted leaves of the mutant and wild-type leaves, whereas transcripts of OsTPX were transcribed at lower levels in the spotted leaves as compared to the non-spotted leaves. The genetic nature of the bl2 mutant indicated that the F1 plants evidenced the wild-type phenotype and that bl2 was governed by a single recessive gene.

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Authors and Affiliations

  1. Molecular Genetics Laboratory, School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Korea

    Mohammad Nurul Matin, Saifullah Ahmed Saief, Mohammad Mominur Rahman & Sang Gu Kang

  2. National Institute of Horticultural and Herbal Science, RDA, Jeju, 697-943, Korea

    Dong Hoon Lee

  3. Department of Biological and Environmental Science, College of Life Science and Biotechnology, Dongguk University, Seoul, 100-715, Korea

    Hoduck Kang

  4. Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry, Yunnan Agricultural University, Kunming, 650201, China

    Dong Sun Lee

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  1. Mohammad Nurul Matin
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  2. Saifullah Ahmed Saief
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  5. Hoduck Kang
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  6. Dong Sun Lee
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Correspondence to Sang Gu Kang.

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Matin, M.N., Saief, S.A., Rahman, M.M. et al. Comparative phenotypic and physiological characteristics of spotted Leaf 6 (spl6) and brown leaf Spot2 (bl2) Lesion Mimic Mutants (LMM) in rice. Mol Cells 30, 533–543 (2010). https://doi.org/10.1007/s10059-010-0151-7

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