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The calmodulin-binding transcription factor OsCBT suppresses defense responses to pathogens in rice

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Molecules and Cells

Abstract

We previously isolated the OsCBT gene, which encodes a calmodulin (CaM)-binding protein, from a rice expression library constructed from fungal elicitor-treated rice suspension cells. In order to understand the function of OsCBT in rice, we isolated and characterized a T-DNA insertion mutant allele named oscbt-1. The oscbt-1 mutant exhibits reduced levels of OsCBT transcripts and no significant morphological changes compared to wild-type plant although the growth of the mutant is stunted. However, oscbt-1 mutants showed significant resistance to two major rice pathogens. The growth of the rice blast fungus Magnaporthe grisea, as well as the bacterial pathogen Xanthomonas oryzae pv. oryzae was significantly suppressed in oscbt-1 plants. Histochemical analysis indicated that the hypersensitive-response was induced in the oscbt-1 mutant in response to compatible strains of fungal pathogens. OsCBT expression was induced upon challenge with fungal elicitor. We also observed significant increase in the level of pathogenesis-related genes in the oscbt-1 mutant even under pathogen-free condition. Taken together, the results support an idea that OsCBT might act as a negative regulator on plant defense.

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Authors and Affiliations

  1. Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, Korea

    Sung Cheol Koo, Hyun Jin Chun, Kyu Young Kang, Dae-Jin Yun, Woo Sik Chung, Moo Je Cho & Min Chul Kim

  2. National Institute of Crop Science, Rural Development Administration, Suwon, 441-857, Korea

    Man Soo Choi, Dong Bum Shin, Yul Ho Kim & Hyang-Mi Park

  3. Department of Plant Science, Seoul National University, Seoul, 151-742, Korea

    Bong Soo Park & Hak Soo Seo

  4. School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Korea

    Jong Tae Song

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  1. Sung Cheol Koo
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  2. Man Soo Choi
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  14. Min Chul Kim
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Correspondence to Min Chul Kim.

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Koo, S.C., Choi, M.S., Chun, H.J. et al. The calmodulin-binding transcription factor OsCBT suppresses defense responses to pathogens in rice. Mol Cells 27, 563–570 (2009). https://doi.org/10.1007/s10059-009-0081-4

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  • DOI: https://doi.org/10.1007/s10059-009-0081-4

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