Abstract
A partial cDNA clone (CG-1) encoding a sequence-specific DNA-binding protein (CG-1) was isolated from a parsley (Petroselinum crispum L.) cDNA expression library by a DNA-ligand screening. The nucleotide sequence CGCG is a required motif in this protein's binding site. Interestingly, the mRNA coding for CG-1 accumulated rapidly and transiently in parsley cultured cells after treatment with UV-containing white light. Although the target gene(s) for CG-1 has not been identified, its sequence-specific DNA binding and expression pattern, make CG-1 a possible member of a light signal transduction chain in parsley.
References
Quail PH: Phytochrome: a light-activated molecular switch that regulates plant gene expression. Annu Rev Genet 25: 389–409 (1991).
Weisshaar B, Armstrong GA, Block A, da Costa e Silva O, Hahlbrock K: Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness. EMBO J 10: 1777–1786 (1991).
Schindler U, Menkens AE, Beckman H, Ecker JR, Cashmore AR: Heterodimerization between light-regulated and ubiquitously expressedArabidopsis GBF bZIP proteins. EMBO J 11: 1261–1273 (1992).
Ahmad M, Cashmore AR:HY4 gene ofA. thaliana encodes a protein with characteristics of a blue-light photoreceptor. Nature 366: 162–166 (1993).
Somssich IE, Bollmann J, Hahlbrock K, Kombrink E, Schulz W: Differential early activation of defense-related genes in elicitor-treated parsley cells. Plant Mol Biol 12: 227–234 (1989).
Lois R, Dietrich A, Hahlbrock K, Schulz W: A phenylalanine ammonia-lyase gene from parsley: structure, regulation and identification of elicitor and light responsivecis-acting elements. EMBO J 8: 1641–1648 (1989).
da Costa e Silva O, Klein L, Schmelzer E, Trezzini GF, Hahlbrock K: BPF-1, a pathogen-induced DNA-binding protein involved in the plant defense response. Plant J 4: 125–135 (1993).
Kawalleck P, Plesch G, Hahlbrock K, Somssich IE: Induction by fungal elicitor ofS-adenosyl-L-methionine synthetase andS-adenosyl-L-homocysteine hydrolase mRNAs in cultured cells and leaves ofPetroselinum crispum. Proc Natl Acad Sci USA 89: 4713–4717 (1992).
Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).
Korfhager U, Trezzini GF, Meier I, Hahlbrock K, Somssich IE: Plant homeodomain protein involved in transcriptional regulation of a pathogen-defense related gene. Plant Cell 6: 695–708 (1994).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Schulze-Lefert P, Dangl JL, Becker-André M, Hahlbrock K, Schulz W: Induciblein vivo DNA footprints define sequences necessary for UV light activation of the parsley chalcone synthase gene. EMBO J 8: 651–656 (1989).
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Silva, O.d.C.e. CG-1, a parsley light-induced DNA-binding protein. Plant Mol Biol 25, 921–924 (1994). https://doi.org/10.1007/BF00028887
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DOI: https://doi.org/10.1007/BF00028887