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Association of Streptococcus mutans collagen binding genes with severe childhood caries

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Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objective

An important factor in the assessment of caries risk is the presence of specific oral microflora, especially Streptococcus mutans. Some S. mutans strains possess proteins capable of binding collagen, such as the Cnm and Cbm proteins. The aim is to determine the presence of S. mutans strains carrying collagen binding proteins in a group of subjects with severe early childhood caries (S-ECC).

Materials and methods

S. mutans strains isolated from 15 S-ECC children were analyzed for collagen binding domains (cbd) of the cnm (cbd/cnm) and cbm (cbd/cbm) genes and their ability to bind to collagen.

Results

S. mutans strains positive for cbd/cnm or cbd/cbm were only found in 3 subjects with the most severe caries profile, with one subject having both cbd/cnm and cbd/cbm, and the other two with one of each. cnm/cbm-positive S. mutans strains bound to collagen substrate more avidly compared with negative S. mutans strains from each of the three groups.

Conclusions

Our findings of an association between the presence of the collagen binding domains of the cnm/cbm genes in plaque S. mutans and the most aggressive form of caries profile in children offer a potential strategy to identify an individual’s risk for caries progression. Our study should be replicated in other settings and communities in longitudinal and longer-term studies.

Clinical relevance

Our data offer a potential tool in the caries risk management and assessment in children.

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Acknowledgments

The authors would like to acknowledge Dr. Ryota Nomura, Osaka University, for the kind gift of the S. mutans strains, NN2193-1 (k); Dr. Aaron Bottner for the recruitment and collection of plaque samples of some of the subjects in the study and to Andrea Sarbu for some of the initial analyses of the plaque samples.

Funding

This study was funded by the University of Toronto Dentistry Research Funds.

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Authors and Affiliations

  1. Faculty of Dentistry, Orthodontics, University of Toronto, 124 Edward Street, Toronto, ON, M2G 1G6, Canada

    Gurkaran S. Lamba, Iacopo Cioffi & Siew-Ging Gong

  2. Faculty of Dentistry, Microbiology, University of Toronto, Toronto, Canada

    Delphine Dufour & Céline M. Lévesque

  3. Faculty of Dentistry, Pediatric Dentistry, University of Toronto, Toronto, Canada

    S. M. Hashim Nainar

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  1. Gurkaran S. Lamba
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  2. Delphine Dufour
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Correspondence to Siew-Ging Gong.

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All procedures performed in this study that involved human participants were in accordance with the ethical standards of the University of Toronto (Research Ethics Board Protocol No.: 32740) and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from guardians of all individual participants included in the study.

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Lamba, G.S., Dufour, D., Nainar, S.M.H. et al. Association of Streptococcus mutans collagen binding genes with severe childhood caries. Clin Oral Invest 24, 3467–3475 (2020). https://doi.org/10.1007/s00784-020-03217-4

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