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The side chain of glutamine 13 is the acyl-donor amino acid modified by type 2 transglutaminase in subunit T of the native rabbit skeletal muscle troponin complex

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Abstract

Subunit T of the native muscle troponin complex is a recognised substrate of transglutaminase both in vitro and in situ with formation of isopeptide bonds. Using a proteomic approach, we have now determined the precise site of in vitro labelling of the protein. A preparation of troponin purified from ether powder from mixed rabbit skeletal muscles was employed as transglutaminase substrate. The only isoform TnT2F present in our preparation was recognised as acyl-substrate by human type 2 transglutaminase which specifically modified glutamine 13 in the N-terminal region. During the reaction, the troponin protein complex was polymerized. Results are discussed in relation to the structure of the troponin T subunit, in the light of the role of troponins in skeletal and cardiac muscle diseases, and to the rules governing glutamine side chain selection by tissue transglutaminase.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Ferrara, Via Borsari 46, 44121, Ferrara, Italy

    Monica Squerzanti, Carlo Cervellati, Blendi Ura, Carlo Mischiati & Carlo M. Bergamini

  2. CEINGE Biotecnologie Avanzate and Department of Organic Chemistry and Biochemistry, University of Naples Federico II, Naples, Italy

    Piero Pucci, Stefano Annunziata & Carla Iannone

  3. Department of Biology, Biocomputing Group, University of Bologna, Bologna, Italy

    Rita Casadio

  4. Department of Chemistry, University of Salerno, Baronissi, SA, Italy

    Carla Esposito

Authors
  1. Monica Squerzanti
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  2. Carlo Cervellati
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  3. Blendi Ura
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  4. Carlo Mischiati
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  5. Piero Pucci
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  6. Stefano Annunziata
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  7. Carla Iannone
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  8. Rita Casadio
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  9. Carlo M. Bergamini
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  10. Carla Esposito
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Corresponding author

Correspondence to Carlo M. Bergamini.

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Squerzanti, M., Cervellati, C., Ura, B. et al. The side chain of glutamine 13 is the acyl-donor amino acid modified by type 2 transglutaminase in subunit T of the native rabbit skeletal muscle troponin complex. Amino Acids 44, 227–234 (2013). https://doi.org/10.1007/s00726-011-1144-3

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  • DOI: https://doi.org/10.1007/s00726-011-1144-3

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