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Molecular evidence does not support the current division of Orthotrichum subgenus Gymnoporus

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Abstract

Eight Orthotrichum species of subgenus Gymnoporus were compared using the internally transcribed spacer regions-1 and -2 and the chloroplast trnH-psbA region. A phylogenetic analysis did not reflect the current division of this subgenus into sections Affinia and Leiocarpa. The investigated sequences revealed a close relationship between O. striatum, a typical species of section Leiocarpa and O. affine, a typical species of section Affinia. An easily distinguishable group was formed by samples of the dioecious O. lyellii, placed into section Leiocarpa. A large number of fixed differences between O. lyellii and other species of subgenus Gymnoporus raises doubts concerning its position within this subgenus. No marker mutations enabling to differentiate O. fastigiatum from O. affine have been found. In absence of such mutations for O. affine and O. striatum, the status of O. fastigiatum cannot be determined unambiguously.

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Authors and Affiliations

  1. Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-727, Olsztyn, Poland

    Jakub Sawicki & Monika Szczecińska

  2. Department of Biology and Ecology, University of Ostrava, Chittussiho 10, 71000, Ostrava, Czech Republic

    Vítězslav Plášek

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  1. Jakub Sawicki
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Correspondence to Jakub Sawicki.

Appendix 1

Appendix 1

Accession data for plants included in the molecular analysis of Orthotrichum subg. Gymnoporus. GenBank accession numbers are given in the following sequence: ITS1, ITS2, trnH-psbA.

O. affine Schrad. ex Brid., Opava-4, Czech Republic: Pivon, FJ159248, FJ168668, FJ036876;

O. affine Schrad. ex Brid., Opava-9, Czech Republic: Bohemia, EU860400, EU072690, FJ036878

O. affine Schrad. ex Brid., Opava-5, Poland: Bialskie Mts, FJ159249, FJ168669, FJ036877

O. fastigiatum Bruch ex Brid., Opava-6, Czech Republic: Cesky Les, FJ159253, FJ168671, FJ036880

O. fastigiatum Bruch ex Brid., Opava-7, Czech Republic: Morava, EU860401, EU072692, FJ036881

O. fastigiatum Bruch ex Brid., Opava-8, Czech Republic: Silesia, FJ159254, FJ168672, FJ036882

O. fastigiatum Bruch ex Brid., Opava-10, Czech Republic: Jasenik, FJ159251, FJ168673, FJ036883

O. fastigiatum Bruch ex Brid., NYBG-36, Armenia: Razdan, FJ159252, FJ168674, FJ036884

O. lyellii Hook. et Taylor, Opava-12, Slovakia: Poloniny MTS, EU863206, EU072689, FJ036872

O. lyellii Hook. et Taylor, Opava-13, Czech Republic: Bohemia, FJ159245, FJ168679, FJ036873

O. lyellii Hook. et Taylor, OLS-14, Poland: Tuczki, FJ159246, FJ168680, FJ036874

O. lyellii Hook. et Taylor, NYBG-42, France: Herault, FJ159247, FJ168681, FJ036875

O. pulchellum Brunt., NYBG-00462757, USA: Clallam Country, EU443996, EU484065, FJ036888

O. pylaisii Brid., NYBG-00151764, Greenland: Godthab, EU863210, EU871637, FJ036869

O. pylaisii Brid., NYBG-00151773, Alaska: Simeonof Island, FJ159260, FJ168667, FJ036868

O. sordidum Sull. et Lesq., NYBG-54, Armenia: Yerevan, EU863212, EU871639, FJ036871

O. sordidum Sull. et Lesq., NYBG-00760280, USA: Connecticut, FJ159261, FJ168666, FJ036870

O. speciosum Nees, Opava-1, Czech Republic: Bohemia, EU863213, EU072695, FJ036863

O. speciosum Nees, Opava-2, Poland: Bialskie Mts, FJ159258, FJ168663, FJ036866

O. speciosum Nees, Opava-3, Slovakia: Liptovice, FJ159257, FJ168664, FJ036865

O. speciosum Nees, Opava-11, Czech Republic: Silesia, FJ159259, FJ168665, FJ036864

O. stramineum Hornsch. ex Brid., Opava-19, Slovakia: Durkovice, FJ159243, FJ168678, FJ036889

O. stramineum Hornsch. ex Brid., Opava-20, Poland: Bialskie Mts, FJ159244, FJ168677, FJ036890

O. stramineum Hornsch. ex Brid., Opava-21, Czech Republic: Bohemia, EU443999, EU072696,

FJ036891

O. striatum Hedw., Opava-153827, Czech Republic: Moravia, EU443993, EU072697,FJ036885

O. striatum Hedw., Opava-16, Poland: Bialskie Mts, FJ159256, FJ168676, FJ036886

O. striatum Hedw., Opava-17, Slovakia: Liptovice, FJ159255, FJ168675, FJ036887

O. vladikavkanum Venturi, NYBG-50, Tajikistan: Dushanbe, EU863214, EU871640, FJ036867

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Sawicki, J., Plášek, V. & Szczecińska, M. Molecular evidence does not support the current division of Orthotrichum subgenus Gymnoporus . Plant Syst Evol 279, 125–137 (2009). https://doi.org/10.1007/s00606-009-0153-0

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