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Taurine attenuates lung ischemia–reperfusion injury after lung transplantation in rats

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Abstract

Purpose

Taurine, the major intracellular free amino acid found in high concentrations in mammalian cells, is known to be an endogenous antioxidant and a membrane-stabilizing agent. It was hypothesized that taurine may be effective in reducing ischemia–reperfusion injury after lung transplantation and an experimental study was conducted in a rat model.

Methods

The number of Sprague–Dawley rats used in the study was 35. Animals were randomized into five groups of 7 rats each, including control, donor I, donor II, ischemia–reperfusion injury, and treatment groups. All animals were exposed to the same experimental conditions in the preoperative period. Rats were fixed in a supine position after the induction. After the rats were shaved, a left pneumonectomy was performed following sternotomy in control, donor I, and donor II groups. The harvested grafts in donor I and donor II groups were transplanted to the rats of the ischemia–reperfusion group and treatment group, respectively. However, taurine was administered intraperitoneally for 3 days before the harvesting procedure in donor II. All harvested lungs were kept in a Euro-Collins solution at +4 °C for 24 h in a half-inflated manner. After harvesting and transplantation, lungs were sampled for histopathological and biochemical analysis.

Results

Malondialdehyde and superoxide dismutase, glutathione peroxidase, and catalase levels were lower in the treatment group than the other groups (p < 0.05). Histopathological findings were better in treatment group than the ischemia–reperfusion group (p < 0.05).

Conclusion

It was demonstrated that donor treatment with taurine resulted in preservation of transplanted lung tissue in respect to histopathological and biochemical findings.

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References

  1. Hosenpud JD, Bennett LE, Keck BM, Fiol B, Boucek MM, Novick RJ. The Registry of the International Society for Heart and Lung Transplantation: sixteenth official report–1999. J Heart Lung Transplant: Off Publ Int Soc Heart Transplant. 1999;18(7):611–26 PubMed PMID: 10452337. Epub 1999/08/19. eng.

    Article  CAS  Google Scholar 

  2. Welbourn CR, Goldman G, Paterson IS, Valeri CR, Shepro D, Hechtman HB. Pathophysiology of ischaemia reperfusion injury: central role of the neutrophil. British J Surg. 1991;78(6):651–5 PubMed PMID: 2070226. Epub 1991/06/01. eng.

    Article  CAS  Google Scholar 

  3. Fischer S, Maclean AA, Liu M, Kalirai B, Keshavjee S. Inhibition of angiotensin-converting enzyme by captopril: a novel approach to reduce ischemia–reperfusion injury after lung transplantation. J Thoracic Cardiovasc Surg. 2000;120(3):573–80 PubMed PMID: 10962421. Epub 2000/08/30. eng.

    Article  CAS  Google Scholar 

  4. Inci I, Dutly A, Rousson V, Boehler A, Weder W. Trimetazidine protects the energy status after ischemia and reduces reperfusion injury in a rat single lung transplant model. J Thorac Cardiovasc Surg. 2001;122(6):1155–61 PubMed PMID: 11726890. Epub 2001/12/01. eng.

    Article  CAS  PubMed  Google Scholar 

  5. Inci I, Inci D, Dutly A, Boehler A, Weder W. Melatonin attenuates post-transplant lung ischemia-reperfusion injury. Annals Thorac Surg. 2002;73(1):220–5 PubMed PMID: 11834013. Epub 2002/02/09. eng.

    Article  Google Scholar 

  6. Kim JD, Baker CJ, Roberts RF, Darbinian SH, Marcus KA, Quardt SM, Starnes VA, Barr ML. Platelet activating factor acetylhydrolase decreases lung reperfusion injury. Annals Thorac Surg. 2000;70(2):423–8. PubMed PMID: 10969656. Epub 2000/09/02. eng.

    Google Scholar 

  7. Kuwaki K, Komatsu K, Sohma H, Abe T. Improvement of ischaemia-reperfusion injury by lazaroid U74389G in rat lung transplantation model. Scand Cardiovasc J. 2000;34(2):209–12 PubMed PMID: 10872712. Epub 2000/06/29. eng.

    Article  CAS  PubMed  Google Scholar 

  8. Mathias MA, Tribble CG, Dietz JF, Nguyen RP, Shockey KS, Kern JA, Kron IL. Aprotinin improves pulmonary function during reperfusion in an isolated lung model. Annals Thorac Surg. 2000;70(5):1671–4. PubMed PMID: 11093508. Epub 2000/11/28. eng.

    Google Scholar 

  9. Kincius M, Liang R, Nickkholgh A, Hoffmann K, Flechtenmacher C, Ryschich E, Gutt CN, Gebhard MM, Schmidt J, Büchler MW, Schemmer P. Taurine protects from liver injury after warm ischemia in rats: the role of kupffer cells. Eur Surg Res Europaische chirurgische Forschung Recherches chirurgicales europeennes. 2007;39(5):275–83. PubMed PMID: 17519554. Epub 2007/05/24. eng.

    Google Scholar 

  10. Shiny KS, Kumar SH, Farvin KH, Anandan R, Devadasan K. Protective effect of taurine on myocardial antioxidant status in isoprenaline-induced myocardial infarction in rats. J Pharm Pharmacol. 2005;57(10):1313–7 PubMed PMID: 16259760. Epub 2005/11/02. eng.

    Article  CAS  PubMed  Google Scholar 

  11. Sahin MA, Yucel O, Guler A, Doganci S, Jahollari A, Cingoz F, Arslan S, Gamsizkan M, Yaman H, Demirkilic U. Is there any cardioprotective role of Taurine during cold ischemic period following global myocardial ischemia? J Cardiothorac Surg. 2011;6:31. PubMed PMID: 21418563. Pubmed Central PMCID: PMC3068941. Epub 2011/03/23. eng.

    Google Scholar 

  12. Yucel O, Kunak ZI, Macit E, Gunal A, Gozubuyuk A, Gul H, Genc O. Protective efficacy of taurine against pulmonary edema progression: experimental study. J Cardiothorac Surg. 2008;3:57. PubMed PMID: 18957088. Pubmed Central PMCID: PMC2583976. Epub 2008/10/30. eng.

  13. Yücel O, Sirin YS, Guler A, Gamsızkan M, Sahin MA, Eken A, Arslan S, Çubuk S, Genç O. Easy applicable model of ischemia and reperfusion on lung transplantation (in Turkish with English abstract). J Clin Anal Med. 2010;1(1):13–20

    Google Scholar 

  14. Al-Fawaeir S, Akgül EÖ, Çaycı T, Demirin H, Kurt YG, Aydın İ, Agilli M, Ozkan E, Yaman H, Cakır E, Erbil MK. Comparison of two Methods for Malondialdehyde Measurement. J Clin Anal Med. 2011;2:11-4.

    Google Scholar 

  15. Eken A, Aydin A, Sayal A, Ustundag A, Duydu Y, Dundar K. The effects of hyperbaric oxygen treatment on oxidative stress and SCE frequencies in humans. Clin Biochem. 2005;38(12):1133–7 PubMed PMID: 16226735. Epub 2005/10/18. eng.

    Article  CAS  PubMed  Google Scholar 

  16. Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121–6 PubMed PMID: 6727660. Epub 1984/01/01. eng.

    Article  CAS  PubMed  Google Scholar 

  17. Ng CS, Wan S, Yim AP. Pulmonary ischaemia-reperfusion injury: role of apoptosis. Eur Respir J: Off J Eur Soc Clin Respir Physiol. 2005;25(2):356–63 PubMed PMID: 15684303. Epub 2005/02/03. eng.

    Article  CAS  Google Scholar 

  18. Huang Y, Shan J, Wang C, Ma J, Li D, Li L, Li S, Li Y. Can ischemic preconditioning alone really protect organs from ischemia reperfusion injury in transplantation. Transplant Immunol. 2009;20(3):127–31. PubMed PMID: 18761408. Epub 2008/09/02. eng.

    Google Scholar 

  19. Schemmer P, Liang R, Kincius M, Flechtenmacher C, Bunzendahl H, Gutt CN, Mehrabi A, Gebhard MM, Büchler MW, Kraus TW. Taurine improves graft survival after experimental liver transplantation. Liver Transplant: Off Publ Am Assoc Study Liver Dis Int Liver Transplant Soc. 2005;11(8):950–9. PubMed PMID: 16035074. Epub 2005/07/22. eng.

  20. Guan X, Dei-Anane G, Liang R, Gross ML, Nickkholgh A, Kern M, Ludwig J, Zeier M, Büchler MW, Schmidt J, Schemmer P. Donor preconditioning with taurine protects kidney grafts from injury after experimental transplantation. The J Surg Res. 2008;146(1):127–34. PubMed PMID: 18061615. Epub 2007/12/07.eng.

    Google Scholar 

  21. Dreyer N, Muhlfeld C, Fehrenbach A, Pech T, von Berg S, Nagib R, Richter J, Wittwer T, Wahlers T, Ochs M. Exogenous surfactant application in a rat lung ischemia reperfusion injury model: effects on edema formation and alveolar type II cells. Respir Res. 2008;9:5. PubMed PMID: 18205928. Pubmed Central PMCID: PMC2265285. Epub 2008/01/22. eng.

    Google Scholar 

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Acknowledgments

Leyla Guler, Murat Tavlasoglu, Orhan Yücel, Adem Güler, and Mehmet Ali Sahin contributed to the pre-operative and operative planning of the groups, and performed the surgical procedures. Mustafa Kurkluoglu, Yusuf Sirin, Ayse Eken, Mehmet Gamsizkan, Mehmet Dakak, Sedat Gurkok, and Onur Genc recorded and analyzed follow-up data. All authors read and approved the final version of manuscript.

Author information

Authors and Affiliations

  1. Department of Anaesthesiology, Gazi University, Ankara, Turkey

    Leyla Guler

  2. Department of Cardiovascular Surgery, Diyarbakir Military Medical Hospital, Yenişehir, 21100, Diyarbakir, Turkey

    Murat Tavlasoglu

  3. Department of Thoracic Surgery, Gülhane Military Medical Academy, Ankara, Turkey

    Orhan Yucel, Mehmet Dakak, Sedat Gurkok & Onur Genc

  4. Cardiac and Vascular Surgery, Gülhane Military Medical Academy, Ankara, Turkey

    Adem Guler & Mehmet Ali Sahin

  5. Department of Cardiovascular Surgery, Children’s National Medical Center, Childrenʼs National Heart Institute, Washington, USA

    Mustafa Kurkluoglu

  6. Research Center, Gülhane Military Medical Academy, Ankara, Turkey

    Yusuf Sirin

  7. Department of Biochemistry, Gülhane Military Medical Academy, Ankara, Turkey

    Ayse Eken

  8. Department of Pathology, Gülhane Military Medical Academy, Ankara, Turkey

    Mehmet Gamsizkan

Authors
  1. Leyla Guler
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  2. Murat Tavlasoglu
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Corresponding author

Correspondence to Murat Tavlasoglu.

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Guler, L., Tavlasoglu, M., Yucel, O. et al. Taurine attenuates lung ischemia–reperfusion injury after lung transplantation in rats. J Anesth 28, 347–353 (2014). https://doi.org/10.1007/s00540-013-1741-2

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  • DOI: https://doi.org/10.1007/s00540-013-1741-2

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