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The role of cytokinins on in vitro shoot production in Salix tetrasperma Roxb.: a tree of ecological importance

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Abstract

A valuable tropical tree, Salix tetrasperma Roxb. commonly known as Indian willow has been investigated for its in vitro regeneration potential using nodal explants obtained from a 30-year-old elite tree. Agar-solidified Woody Plant Medium (WPM) containing different concentrations of Plant Growth Regulators (PGRs) was used in the study. Shoot induction response was best on WPM supplemented with 6-benzyladenine (5.0 μM) where 90% explants responded with an average shoot number (4.40 ± 0.50) and shoot length (0.92 ± 0.04) after 6 weeks of culture. However, multiplication and elongation was best recorded when BA (5.0 μM) treated shoot clusters were transferred to WPM containing BA (1.0 μM) + NAA (0.5 μM) where 18.40 ± 0.92 well-grown healthy shoots with an average shoot length of 5.30 ± 0.43 cm were obtained on completion of 12 weeks culture period. In vitro rooting of shoots was best achieved in half-strength WPM containing 0.5 μM IBA. Well-rooted plantlets were successfully hardened off and acclimatized in plastic cups containing sterile Soilrite. These plantlets were then transferred to pots containing normal garden soil followed by transfer to greenhouse and ultimately to field under full sun.

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Abbreviations

BA:

6-Benzylaminopurine

NAA:

α Naphthalene acetic acid

WPM:

Woody plant medium

PGRs:

Plant growth regulators

References

  • Agrawal DC, Gebhardt K (1994) Rapid micropropagation of hybrid willow (Salix) established by ovary culture. J Plant Physiol 143:763–765

    CAS  Google Scholar 

  • Ahmad N, Anis M (2007) Rapid clonal multiplication of a woody tree, Vitex negundo L. through axillary shoots proliferation. Agroforest Syst 71:195–200

    Article  Google Scholar 

  • Ahmad N, Anis M (2010) An efficient in vitro process for recurrent production of cloned plants of Vitex negundo L. Eur J For Res. doi: 10.1007/s10342-010-0415-y

  • Ahmad N, Wali SA, Anis M (2008) In vitro production of true-to-type plants of Vitex negundo L. from nodal explants. J Hortic Sci Biotech 83(3):313–317

    CAS  Google Scholar 

  • Ahuja MR (1993) Regeneration and germplasm in aspen—Populus. In: Ahuja MR (ed) Micropropagation of woody plants. Kluwer, Dordrecht, pp 187–194

    Google Scholar 

  • Amo Marco JB, Lledo MD (1996) In vitro propagation of Salix tarraconensis Pau ex Font Quer, an endemic and threatened plant. In Vitro Cell Dev Biol Plant 32:42–46

    Article  Google Scholar 

  • Anis M, Husain MK, Faisal M, Shahzad A, Ahmad N, Siddique I, Khan H (2009) In vitro approaches for plant regeneration and conservation of some medicinal plants. In: Kumar A, Sopory SK (eds) Recent advances in plant biotechnology and its applications, vol 14. IK International Pvt Ltd, New Delhi, pp 397–410

    Google Scholar 

  • Anis M, Varshney A, Siddique I (2010) In vitro clonal propagation of Balanites aegyptiaca (L.) Del. Agroforest Syst 78:151–158

    Article  Google Scholar 

  • Anonymous (2003) The wealth of India: a dictionary of Indian raw materials and industrial products, CSIR, New Delhi, 169–175

  • Bergman L, von Arnold S, Erickson T (1985) Effects of N6-benzyladenine on shoots of five willow clones (Salix spp) cultured in vitro. Plant Cell Tissue Organ Cult 4:135–144

    Article  Google Scholar 

  • Bhojwani SS (1980) Micropropagation method for a hybrid willow (Salix matsudana X alba NZ-1002). N Z J Bot 18:209–214

    Google Scholar 

  • Chalupa V (1983) Micropropagation of conifer and broad leaved forest trees. Commun Instit Forest Cechoslov 13:7–39

    Google Scholar 

  • Dhir KK, Angrish R, Bajaj M (1984) Micropropagation of Salix babylonica through in vitro shoot proliferation. Proc Indian Acad Sci (Plant Sci) 93(6):655–660

    Google Scholar 

  • Faisal M, Anis M (2002) Rapid in vitro propagation of Rauvolfia tetraphylla L.—an endangered medicinal plant. Physiol Mol Biol Plants 8:295–299

    Google Scholar 

  • Faisal M, Ahmad N, Anis M (2007) An efficient micropropagation system for Tylophora indica: an endangered, medicinally important plant. Plant Biotechnol Rep 1:765–772

    Article  Google Scholar 

  • Gebhardt K (1992) Grudlagen der Zuhtung pharmazeutisch wertvoller Weiden. Die Holzzucht 46:9–14

    Google Scholar 

  • Giri CC, Shyamkumar B, Anjaneyulu C (2004) Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview. Trees 18:115–135

    Google Scholar 

  • Granel T, Robinson B, Mills T, clothier B, Green S, Fung L (2002) Cadmium accumulation by willow clones used for soil conservation, stock fodder, and phytoremediation. Aust J Soil Res 40:1331–1337

    Article  CAS  Google Scholar 

  • Grönroos L, von Arnold S, Erikson T (1989) Callus production and somatic embryogenesis from floral explants of basket willow (Salix viminalis L.). J Plant Physiol 134:558–566

    Google Scholar 

  • Husain MK, Anis M (2009) Rapid in vitro multiplication of Melia azaderach L. (A multipurpose woody tree). Acta Physiol Plant 31:765–772

    Article  CAS  Google Scholar 

  • Johansen DA (1940) Plant microtechnique. McGraw-Hill, New York, pp 126–154

    Google Scholar 

  • Khalafalla MM, Hattori K (2000) Ethylene inhibitors enhance in vitro root formation on faba bean shoots regenerated on medium containing thidiazuron. Plant Growth Regul 32:59–63

    Article  CAS  Google Scholar 

  • Kuzovkina YA, Quigley MF (2005) Willows beyond wetlands: uses of Salix species for environmental projects. Water Air Soil Pollut 162:183–204

    Article  CAS  Google Scholar 

  • Lloyd G, Mc Cown B (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Proc Int Plant Propag Soc 30:421–427

    Google Scholar 

  • Lyyra S, Lima A, Merkle SA (2006) In vitro regeneration of Salix nigra from adventitious shoots. Tree Physiol 26:969–975

    PubMed  CAS  Google Scholar 

  • Neuner H, Beiderback R (1993) In vitro propagation of Salix caprea L by single node explants. Silvae Genet 42:308–310

    Google Scholar 

  • Park SY, Kim YW, Moon HK, Murthy HN, Choi YH, Cho HM (2008) Micropropagation of Salix pseudolasiogne from nodal explants. Plant Cell Tissue Organ Cult 93:341–346

    Article  Google Scholar 

  • Perttu KL, Kowalik PJ (1997) Salix vegetation filters for purification of waters and soils. Biomass Bioenergy 12:9–19

    Article  CAS  Google Scholar 

  • Raskin I (1992) Role of salicylic acid in plants. Annu Rev Plant Physiol Mol Biol 43:439–463

    Article  CAS  Google Scholar 

  • Read PE, Garton S, Tormala T (1989) Willows (Salix spp.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Trees II, vol 4. Springer, Berlin, pp 370–386

    Google Scholar 

  • Shukla S, Shukla SK, Mishra SK (2009) In vitro plant regeneration from seedling explants of Stereospermum personatum DC: a medicinal tree. Trees 23:409–413

    Article  Google Scholar 

  • Siddique I, Anis M (2009) Direct plant regeneration from nodal explants of Balanites aegyptiaca L. (Del.)—a valuable medicinal tree. New Forests 37:53–62

    Article  Google Scholar 

  • Stoehr MU, Cai M, Zsuffa L (1989) In vitro plant regeneration via callus culture of mature Salix exigua. Can J For Res 19:1634–1638

    Article  Google Scholar 

  • Thorpe TA, Harry IS, Kumar PP (1991) Application of micropropagation to forestry. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Kluwer, Dordrecht, pp 311–336

    Google Scholar 

  • Vervaeke P, Luyssaert S, Mertens J, Meers E, Tack FMG, Lust N (2003) Phytoremediation prospects of willow stands on contaminated sediment: a field trial. Environ Pollut 126:275–282

    Article  PubMed  CAS  Google Scholar 

  • Vinocur B, Carmi T, Altman A, Ziv M (2000) Enhanced bud regeneration in aspen (Populus tremula L.) roots cultured in liquid media. Plant Cell Rep 19:1146–1154

    Article  CAS  Google Scholar 

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Acknowledgments

Financial assistance to Md Imran Khan in the form of JRF (NET) by Council of Scientific and Industrial Research (CSIR), Govt. of India, New Delhi is gratefully acknowledged. Research support provided by the Department of Science and Technology (DST) and University Grants Commission (UGC) Govt. of India, New Delhi, in the form of DST-FIST (2005) and UGC-SAP (2009) Programmes is duly acknowledged.

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, India

    Md Imran Khan, Naseem Ahmad & Mohammad Anis

  2. Department of Plant Production, College of Food and Agriculture Science, King Saud University, PO Box 2460, Riyadh, 11451, Saudi Arabia

    Mohammad Anis

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  1. Md Imran Khan
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  2. Naseem Ahmad
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  3. Mohammad Anis
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Correspondence to Mohammad Anis.

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Communicated by J. Carlson.

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Khan, M.I., Ahmad, N. & Anis, M. The role of cytokinins on in vitro shoot production in Salix tetrasperma Roxb.: a tree of ecological importance. Trees 25, 577–584 (2011). https://doi.org/10.1007/s00468-010-0534-6

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  • DOI: https://doi.org/10.1007/s00468-010-0534-6

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