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Elevated CO2 concentration and temperature effects on the partitioning of chemical components along juvenile Scots pine stems (Pinus sylvestris L.)

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Abstract

The effects of doubled ambient [CO2] and different temperature levels on young Pinus sylvestris growing in phytotron chambers were studied. Five chambers were supplied with ~380 (‘ambient air’) and five with ~700 μmol mol−1 CO2 (‘elevated [CO2]’). Temperature levels in the chambers ranged in increment steps of 2°C from −4°C to +4°C relative to the long-term monthly (day and night) average air temperature levels in Berlin–Dahlem. Substrate was medium fertile; soil moisture and air humidity were kept constant. After three vegetation periods twigs and stems were harvested, weighed, homogenized, and analyzed chemically. There was no significant temperature effect on wood mass accumulation, clearest positive [CO2] effect occurred in the youngest twigs. In total, wood mass increased by 28.5% at doubled ambient [CO2]. N-contents (percentage) decreased at elevated [CO2] in the uppermost stem sections and not in twig wood causing wider C/N ratios in total. In response to elevated temperature, N-contents decreased slightly in twigs (~0.3%). Traces of free glucose, fructose and sucrose, which decreased from the top to the bottom, were found in stem wood, in contrast to traces of starch that increased from the top to the bottom. In response to elevated [CO2] only a little more (0.05%) was accumulated in the top shoot and in tendency; glucose, fructose, and sucrose contents were lower at the bottom of stems as compared to the control. There was no obvious response of these non-structural carbohydrates to elevated temperature except for starch that decreased to half of the content from the lowest to the highest temperature level. Among the hemicellulose compounds, rhamnose and arabinose declined from the top shoot to the bottom of stem, whereas 4-O-methyl-d-glucuronic-acid, mannose, and xylose increased. Contents (percentage) of galactose remained approximately stable along the stem. The clearest positive effect of elevated [CO2] along the whole stem was found for mannose with differences of 0.6–0.3%. In contrast to rhamnose and arabinose that showed a negative response to elevated [CO2], mannose was reduced towards the uppermost stem sections. The 4-O-methyl-d-glucuronic-acid was slightly lowered at the bottom, and galactose and xylose showed no [CO2] response. The only hemicellulose compound which reacted to temperature elevation was galactose. It increased slightly (~0.1% per 1°C). Cellulose and lignin (Klason) behaved oppositely: cellulose increased and lignin decreased from the top to the bottom. These structural components behaved reversely also in response to elevated [CO2]. In stem parts above the bottom section, cellulose content was slightly higher at elevated [CO2], and lignin content was slightly lower at the bottom. Lignin reacted to temperature elevation by a very slight increase on the average (~0.1% per one 1°C). Cellulose, however, decreased by ~0.2% per 1°C temperature elevation. The importance of persistent sinks of carbon in woody plant parts is discussed in respect to the greenhouse effect.

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Acknowledgments

The research was funded by the 5th Framework programme of the European Commission through the MEFYQUE research contract QLKS-CT-2004. Special thanks are addressed to Dr. J. Puls from the Institute of Wood Chemistry and Chemical Technology of Wood/Research Centre for Forestry and Wood-Economy in Hamburg, Germany. He contributed know-how, use of instruments and many analyses especially to the part about hemicellulose components, cellulose and lignin. Language was ‘polished’ by Sally Johnson.

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  1. TU-Berlin, Institut für Ökologie/Ökologie der Gehölze, Königin-Luise-Str. 22, 14195, Berlin, Germany

    Dieter Overdieck & Karin Fenselau

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  1. Dieter Overdieck
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Correspondence to Dieter Overdieck.

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Communicated by U. Lüttge.

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Overdieck, D., Fenselau, K. Elevated CO2 concentration and temperature effects on the partitioning of chemical components along juvenile Scots pine stems (Pinus sylvestris L.). Trees 23, 771–786 (2009). https://doi.org/10.1007/s00468-009-0319-y

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