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Establishment of an immortalized cell line derived from the pupal ovary of Mythimna separata (Lepidoptera: Noctuidae) and identification of the cell source

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Abstract

Determining the source of primary cells is conductive to enriching sufficient cells with immortal potential thereby improving the success rate of establishing cell lines. However, most of the existing insect cell lines are established by mixing and fragmentation of explants. At present, the origin of cell lines can only be determined according to the cultured tissues, so it is impossible to determine which cell types they come from. In this study, a new cell line designated IOZCAS-Myse-1 was generated from pupal ovaries of the migratory pest Mythimna separata by explant tissues to derive adherent cultures. This paper mainly shows the further descriptive information on the origin of primary cells in the process of ovarian tissue isolation and culture. Phospho-histone H3 antibody-labeled cells with mitotic activity showed that the rapidly developing somatic cells in vivo gradually stopped proliferation when cultured ex vivo. The primary cells dissociated outside the tissue originated from the lumen cells, rather than the germ cells or the follicular epithelium cells. The results suggest that the newly established cell line IOZCAS-Myse-1 had two possible sources. One is the mutation of lumen cells in the vitellarium, and the other is the stem cells with differentiation potential in the germarium of the ovarioles. Moreover, the newly established cell line is sensitive to the infection of Autographa californica multiple nucleopolyhedrovirus, responds to 20-hydroxyecdysone and has weak encapsulation ability. Therefore, the new cell line can be a useful platform for replication of viral insecticides, screening of hormone-based insecticides and immunology research.

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Acknowledgements

We thank Prof. Chang-You Li for providing cell line QAU-Se-E-3 established from embryo of Spodoptera exigua.

Funding

This work was supported by the National Nature Science Foundation of China (Project No. 31671404), and the Key Research Program of the Chinese Academy of Sciences (No. KJZD-SW-L07).

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Authors and Affiliations

  1. State Key Laboratory of Integrated Management of Pests and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China

    Yan Tong, Lu-Qiang Cheng, Xuan Li, Xu-Peng Yu, Rui-Hao Shu, Ji-Hong Zhang, Qian Meng, Qi-Lian Qin, Kai Tang & Huan Zhang

  2. Institutes of Physical Science and Information Technology, Anhui University, Hefei, China

    Yan Tong

  3. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China

    Lu-Qiang Cheng & Jian-Xiang Xu

  4. University of Chinese Academy of Sciences, Beijing, China

    Yan Tong, Xu-Peng Yu & Rui-Hao Shu

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  1. Yan Tong
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  3. Xuan Li
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Correspondence to Jian-Xiang Xu or Huan Zhang.

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Tong, Y., Cheng, LQ., Li, X. et al. Establishment of an immortalized cell line derived from the pupal ovary of Mythimna separata (Lepidoptera: Noctuidae) and identification of the cell source. Cell Tissue Res 386, 661–677 (2021). https://doi.org/10.1007/s00441-021-03528-2

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