Abstract
Rodents have been widely studied as intermediate hosts of Sarcocystis; however, only a few reports on these parasites in the black rat (Rattus rattus) are known. Having examined 13 black rats captured in Latvia, sarcocysts were found in skeletal muscles of two mammals and were described as Sarcocystis ratti n. sp. Under a light microscope, sarcocysts were ribbon-shaped, 0.9–1.3 × 0.09–0.14 mm in size and had a thin (0.8–1.3 μm) and smooth cyst wall. The lancet-shaped bradyzoites were 8.3 × 4.3 (7.5–9.3 × 3.9–4.8) μm. Under a transmission electron microscope, the cyst wall was up to 1.3 μm thick, wavy, the ground substance appeared smooth, type 1a-like. Morphologically, sarcocysts of S. ratti were somewhat similar to those of S. cymruensis, S. rodentifelis, and S. dispersa-like previously identified in the brown rat (Rattus norvegicus). On the basis of 18S rDNA, 28S rDNA, and cox1, significant genetic differences (at least 2.3, 4.5, and 5.8%, respectively) were observed when comparing S. ratti with other Sarcocystis species using rodents as intermediate hosts. While ITS1 sequences of S. ratti were highly distinct from other Sarcocystis species available in GenBank. Phylogenetic and ecological data suggest that predatory mammals living near households are definitive hosts of S. ratti.
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References
Antunes Murata FH, Cerqueira-Cézar CK, Thompson PC, Tiwari K, Mowery JD, Verma SK, Rosenthal BM, Sharma RN, Dubey JP (2018) Sarcocystis cymruensis: discovery in Western hemisphere in the Brown rat (Rattus norvegicus) from Grenada, West Indies: redescription, molecular characterization, and transmission to IFN-γ gene knockout mice via sporocysts from experimentally infected domestic cat (Felis catus). Parasitol Res 117:1195–1204. https://doi.org/10.1007/s00436-018-5799-5
Beaver PC, Maleckar JR (1981) Sarcocystis singaporensis Zaman and Colley, (1975) 1976, Sarcocystis villivilliso sp. n., and Sarcocystis zamani sp. n.: development, morphology, and persistence in the laboratory rat, Rattus norvegicus. J Parasitol 67:241–256
Burnie D, Wilson DE (2006) Animal: the definitive visual guide. Mammals Part I. Dorling Kindersley Limited, London
Dolezel D, Koudela B, Jurků M, Hypsa V, Obornik M, Votýpka J, Modrý D, Slapeta JR, Lukes J (1999) Phylogenetic analysis of Sarcocystis spp. of mammals and reptiles supports the coevolution of Sarcocystis spp. with their final hosts. Int J Parasitol 29:795–798. https://doi.org/10.1016/S0020-7519(99)00018-1
Dubey JP, Cawthorn RJ, Speer CA, Wobeser GA (2003) Redescription of the sarcocysts of Sarcocystis rileyi (Apicomplexa: Sarcocystidae). J Eukaryot Microbiol 50:476–482. https://doi.org/10.1111/j.1550-7408.2003.tb00274.x
Dubey JP, Verma SK, Dunams D, Calero-Bernal R, Rosenthal BM (2015) Molecular characterization and development of Sarcocystis speeri sarcocysts in gamma interferon gene knockout mice. Parasitology 142:1555–1562. https://doi.org/10.1017/S0031182015001109
Dubey JP, Calero-Bernal R, Rosenthal BM, Speer CA, Fayer R (2016) Sarcocystosis of animals and humans, 2nd edn. CRC Press, Boca Raton
Gjerde B (2013) Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome c oxidase subunit I gene. Int J Parasitol 43:579–591. https://doi.org/10.1016/j.ijpara.2013.02.004
Gjerde B (2014) Molecular characterisation of Sarcocystis rileyi from a common eider (Somateria mollissima) in Norway. Parasitol Res 113:3501–3509. https://doi.org/10.1007/s00436-014-4062-y
Gjerde B, Vikøren T, Hamnes IS (2018) Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway. Int J Parasitol Parasites Wildl 7:1–11. https://doi.org/10.1016/j.ijppaw.2017.12.001
Grikienienė J, Arnastauskienė T, Kutkienė L (1993) On some disregarded ways of Sarcosporidians’ circulation and remarks about systematics of the genus Sarcocystis Lankester, 1882 with the description of the new species from rodents. Ekologija 1:16–24
Grikienienė J, Malakauskas M, Mažeikytė R, Balčiauskas L, Senutaitė J (2001) Muscle parasites (Sarcocystis, Trichinella, Alaria) of wild mammals in Lithuania. Theriol Lituanica 1:29–46
Hu JJ, Ma TC, Li XR (2005) A new species of sarcocysts (Sporozoea, Eucoccidiida) from Rattus norvegicus. Acta Zootaxon Sin 30:287–290. https://doi.org/10.1645/GE-2831.1
Hu JJ, Liao JY, Meng Y, Guo YM, Chen XW, Zuo YX (2011) Identification of Sarcocystis cymruensis in wild Rattus flavipectus and Rattus norvegicus from Peoples Republic of China and its transmission to rats and cats. J Parasitol 97:421–424. https://doi.org/10.1645/GE-2633.1
Hu JJ, Meng Y, Guo YM, Liao JY, Song JL (2012) Completion of the life cycle of Sarcocystis zuoi, a parasite from the Norway rat, Rattus norvegicus. J Parasitol 98:550–553. https://doi.org/10.1645/GE-2831.1
Jäkel T, Khoprasert Y, Sorger I, Kliemt D, Seehabutr V, Suasa-ard K, Hongnark S (1997) Sarcosporidiasis in rodents from Thailand. J Wildl Dis 33:860–867. https://doi.org/10.7589/0090-3558-33.4.860
Kampe-Pērsone G (2017) Latvijas zīdītāji. Pilnīgs sugu apraksts. Zvaigzne ABC, Riga
Kan SP, Dissanaike AS (1977) Ultrastructure of Sarcocystis sp. from the Malaysian house rat, Rattus rattus diardii. Z Parasitenkd 52:219–227
Koudela B, Modrý D (2000) Sarcocystis muris possesses both diheteroxenous and dihomoxenous characters of life cycle. J Parasitol 86:877–879. https://doi.org/10.1645/0022-3395(2000)086[0877:SMPBDA]2.0.CO;2
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev/msw054
Kutkienė L, Prakas P, Sruoga A, Butkauskas D (2010) The mallard duck (Anas platyrhynchos) as intermediate host for Sarcocystis wobeseri sp. nov. from the barnacle goose (Branta leucopsis). Parasitol Res 107:879–888. https://doi.org/10.1007/s00436-010-1945-4
Kutkienė L, Prakas P, Butkauskas D, Sruoga A (2012) Description of Sarcocystis turdusi sp. nov. from the common blackbird (Turdus merula). Parasitology 139:1438–1443. https://doi.org/10.1017/S0031182012000819
Matuschka FR (1987) Reptiles as intermediate and/or final hosts of Sarcosporidia. Parasitol Res 73:22–32
Milne I, Wright F, Rowe G, Marshall D, Husmeier D, McGuire G (2004) TOPALi: software for automatic identification of recombinant sequences within DNA multiple alignments. Bioinformatics 20:1806–1807. https://doi.org/10.1093/bioinformatics/bth155
Modrý D, Votýpka J, Svobodová M (2004) Note on the taxonomy of Frenkelia microti (Findlay & Middleton, 1934) (Apicomplexa: Sarcocystidae). Syst Parasitol 58:185–187. https://doi.org/10.1023/b:sypa.0000032924.63708.57
Mugridge NB, Morrison DA, Johnson AM, Luton K, Dubey JP, Votýpka J, Tenter AM (1999) Phylogenetic relationships of the genus Frenkelia: a review of its history and new knowledge gained from comparison of large subunit ribosomal ribonucleic acid gene sequences. Int J Parasitol 29:957–972. https://doi.org/10.1016/S0020-7519(99)00062-4
Mugridge NB, Morrison DA, Jäkel T, Heckeroth AR, Tenter AM, Johnson AM (2000) Effects of sequence alignment and structural domains of ribosomal DNA on phylogeny reconstruction for the protozoan family sarcocystidae. Mol Biol Evol 17:1842–1853. https://doi.org/10.1093/oxfordjournals.molbev.a026285
Munday BL (1977) A species of Sarcocystis using owls as definitive hosts. J Wildl Dis 13:205–207
Munday BL (1983) An isosporan parasite of masked owls producing sarcocysts in rats. J Wildl Dis 19:146–147
Munday BL, Mason RW (1980) Sarcocystis and related organisms in Australian wildlife: III. Sarcocystis murinotechis sp. n. life cycle in rats (Rattus, Pseudomys and Mastocomys spp.) and tiger snakes (Notechis ater). J Wildl Dis 16:83–88
Prakas P, Butkauskas D (2012) Protozoan parasites from genus Sarcocystis and their investigations in Lithuania. Ekologija 58:45–58
Prakas P, Kutkienė L, Butkauskas D, Sruoga A, Žalakevičius M (2014) Description of Sarcocystis lari sp. n. (Apicomplexa: Sarcocystidae) from the great black-backed gull, Larus marinus (Charadriiformes: Laridae), on the basis of cyst morphology and molecular data. Folia Parasitol 61:11–17. https://doi.org/10.14411/fp.2014.002
Prakas P, Butkauskas D, Rudaitytė E, Kutkienė L, Sruoga A, Pūraitė I (2016) Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania. Parasitol Res 115:3021–3032. https://doi.org/10.1007/s00436-016-5057-7
Prakas P, Butkauskas D, Švažas S, Juozaitytė-Ngugu E, Stanevičius V (2018) Morphologic and genetic identification of Sarcocystis fulicae n. sp. (Apicomplexa: Sarcocystidae) from the Eurasian coot (Fulica atra). J Wildl Dis 54:765–771. https://doi.org/10.7589/2017-11-279
Slapeta JR, Modrý D, Koudela B (1999) Sarcocystis atheridis sp. nov., a new sarcosporidian coccidium from Nitsche’s bush viper, Atheris nitschei Tornier, 1902, from Uganda. Parasitol Res 85:758–764
Slapeta JR, Modrý D, Votýpka J, Jurků M, Koudela B, Lukes J (2001) Multiple origin of the dihomoxenous life cycle in sarcosporidia. Int J Parasitol 31:413–417. https://doi.org/10.1016/S0020-7519(01)00127-8
Slapeta JR, Modrý D, Votýpka J, Jurků M, Lukes J, Koudela B (2003) Evolutionary relationships among cyst-forming coccidian Sarcocystis spp. (Alveolata: Apicomplexa: Coccidea) in endemic African tree vipers and perspective for evolution of heteroxenous life cycle. Mol Phylogenet Evol 27:464–475. https://doi.org/10.1016/S1055-7903(03)00018-6
Verma SK, von Dohlen AR, Mowery JD, Cerqueira-Cézar CK, Rosenthal BM, Dubey JP, Lindsay DS (2017) Sarcocystis strixi n. sp. from barred owl (Strix varia) definitive host and interferon gamma gene knockout mice as experimental intermediate host. J Parasitol 103:768–777. https://doi.org/10.1645/16-173
Votýpka J, Hypsa V, Jirků M, Flegr J, Vávra J, Lukes J (1998) Molecular phylogenetic relatedness of Frenkelia spp. (Protozoa, Apicomplexa) to Sarcocystis falcatula Stiles 1893: is the genus Sarcocystis paraphyletic? J Eukaryot Microbiol 45:137–141. https://doi.org/10.1111/j.1550-7408.1998.tb05081.x
Watthanakaiwan V, Sukmak M, Hamarit K, Kaolim N, Wajjwalku W, Muangkram Y (2017) Molecular characterization of the ribosomal DNA unit of Sarcocystis singaporensis, Sarcocystis zamani and Sarcocystis zuoi from rodents in Thailand. J Vet Med Sci 79:1412–1418. https://doi.org/10.1292/jvms.16-0086
Zaman V, Colley FC (1975) Light and electron microscopic observations of the life cycle of Sarcocystis orientalis sp. n. in the rat (Rattus norvegicus) and the Malaysian reticulated python (Python reticulatus). Z Parasitenkd 47:169–185
Zaman V, Colley FC (1976) Replacement of Sarcocystis orientalis Zaman and Colley, 1975, by Sarcocystis singaporensis sp. n. Z Parasitenkd 51:137
Acknowledgements
The authors are grateful to Ms. S. Amšiejienė from the National Centre of Pathology (Vilnius, Lithuania) for her help in carrying out electron microscopy investigations.
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Prakas, P., Kirillova, V., Gavarāne, I. et al. Morphological and molecular description of Sarcocystis ratti n. sp. from the black rat (Rattus rattus) in Latvia. Parasitol Res 118, 2689–2694 (2019). https://doi.org/10.1007/s00436-019-06393-9
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DOI: https://doi.org/10.1007/s00436-019-06393-9