Abstract
Amilenus aurantiacus overwinter in diapause, a natural starvation period, in hypogean habitats. The structure of spherites in the midgut diverticula (MD) and Malpighian tubules (MT) has been studied comparatively by light microscopy and TEM to detect eventual differences in mineral consumption in the beginning and at the end of the starvation period in these organs (MD and MT) associated with digestive processes. The chemical composition of spherites was examined by combining energy-dispersive X-ray spectroscopy (EDXS), electron energy-loss spectroscopy (EELS) and energy-filtered TEM (EFTEM). The structure of the spherites changed during overwintering in both organs. At the beginning of overwintering, the spherites were composed of densely packed concentric layers of electron-dense and electron-lucent material. In the middle and at the end of overwintering, the electron-lucent layers between the layers of material indicated the loss of some material. The chemical composition of the spherites changed only in the MD; at the beginning of overwintering, these contained Si, O, C and Fe, while later there was no more Fe. In contrast, spherites in the MT were composed of Si, O, C and Ca throughout overwintering. A less intensive exploitation of the MD spherites was probably due to complete cessation of digestive and other cell activity in this organ during the winter diapause; activity of the MT slowed, but continued removing the cell metabolites.
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Acknowledgements
We would like to thank Elisabeth Bock and Rudi Schmied (Medical University Graz) for their excellent technical assistance. Michelle Gadpaille provided valuable help in improving the English of the manuscript. We are indebted to two anonymous reviewers for insightful comments and helpful suggestions. This study was partly supported by the Slovenian Research Agency within the Biodiversity Research Programme (Grant No. P1-0078) and by the grant “Infrastrukturni program” University of Maribor (IP-0552).
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Lipovšek, S., Novak, T., Dariš, B. et al. Ultrastructure of spherites in the midgut diverticula and Malpighian tubules of the harvestman Amilenus aurantiacus during the winter diapause. Histochem Cell Biol 157, 107–118 (2022). https://doi.org/10.1007/s00418-021-02046-0
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DOI: https://doi.org/10.1007/s00418-021-02046-0