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Identification of human papillomavirus (HPV) subtype in oral cancer patients through microarray technology

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European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Purpose

Human papilloma virus (HPV) is the main source of cervical cancer. Many recent studies have revealed the prevalence and prognosis of HPV associated with oropharyngeal squamous cell carcinoma, but fewer reports have evaluated HPV in oral squamous cell carcinoma (OSCC). The purpose of this study was to determine the prevalence and prognosis of HPV associated with OSCC according to HPV and tumor types.

Materials and methods

We used a DNA chip kit (MY-HPV chip kit ®, Mygene Co., Korea) to detect high-risk HPV subtypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 54, 56, 58) and low-risk subtypes (6, 11, 34, 40, 42, 43, 44) among 187 patients. The prevalence was determined by Chi-square and Fisher’s exact tests, and the prognosis was calculated by the Kaplan–Meier method and the log-rank test.

Results

The overall prevalence of HPV in OSCC was 7.0% for all HPV positives and 4.3% for high-risk HPV positives. The prevalence of HPV was significantly higher in individuals under 65 years old and in those with tumors in the tongue and gum regions. The prognosis did not differ between the HPV-positive and -negative groups. Although the prevalence of HPV-positive cases in OSCC was low (7.0, 4.3%) and the prognosis did not depend on HPV positivity, HPV-associated OSCC should be considered in the evaluation and treatment of oral cancer patients. In addition, separating high- and low-risk groups based on the HPV status of other body parts might not be appropriate.

Discussion

The DNA microarray method can accurately detect known HPV subtypes simultaneously, but has limitations in detecting new subtypes. Vaccines can also be used to prevent HPV-associated OSCC in patients, so further studies on the prognosis and efficacy of vaccines should be undertaken.

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References

  1. Bosch F, Lorincz A, Munoz N (2002) The causal relation between human papillomavirus and cervical cancer. J Clin Pathol 55:244–265

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Smith EM, Hoffman HT, Summersgill KS et al (1998) Human papillomavirus and risk of oral cancer. Laryngoscope 108:1098–1103

    Article  CAS  PubMed  Google Scholar 

  3. Ndiaye C, Mena M, Alemany L et al (2014) HPV DNA, E6/E7 mRNA, and p16INK4a detection in head and neck cancers: a systematic review and meta-analysis. Lancet Oncol 15:1319–1331

    Article  CAS  PubMed  Google Scholar 

  4. Lingen MW, Xiao W, Schmitt A et al (2013) Low etiologic fraction for high-risk human papillomavirus in oral cavity squamous cell carcinomas. Oral Oncol 49:1–8

    Article  PubMed  Google Scholar 

  5. D’Souza G, Kreimer AR, Viscidi R et al (2007) Case-control study of human papillomavirus and oropharyngeal cancer. N Engl J Med 356:1944–1956

    Article  PubMed  Google Scholar 

  6. Anantharaman D, Abedi-Ardekani B, Beachler DC et al (2017) Geographic heterogeneity in the prevalence of human papillomavirus in head and neck cancer. Int J Cancer 1975:1968–1975

    Article  Google Scholar 

  7. Shin KH, Park KH, Hong HJ et al (2002) Prevalence of microsatellite instability, inactivation of mismatch repair genes, p53 mutation, and human papillomavirus infection in Korean oral cancer patients. Int J Oncol 21:297–302

    CAS  PubMed  Google Scholar 

  8. Kansy K, Thiele O, Freier K (2014) The role of human papillomavirus in oral squamous cell carcinoma: myth and reality. Oral Maxillofac Surg 18:165–172

    Article  PubMed  Google Scholar 

  9. Kim SM (2016) Human papilloma virus in oral cancer. J Korean Assoc Oral Maxillofac Surg 42:327–336

    Article  PubMed  PubMed Central  Google Scholar 

  10. Schwartz SR, Yueh B, McDougall JK et al (2001) Human papillomavirus infection and survival in oral squamous cell cancer: a population-based study. Otolaryngol Head Neck Surg 125:1–9

    Article  CAS  PubMed  Google Scholar 

  11. Westra WH (2014) Detection of human papillomavirus (HPV) in clinical samples: evolving methods and strategies for the accurate determination of HPV status of head and neck carcinomas. Oral Oncol 50:771–779

    Article  PubMed  PubMed Central  Google Scholar 

  12. Lim YK, Choi J-H, Park S et al (2016) Comparison of three different commercial kits for the human papilloma virus genotyping. J Clin Lab Anal 6:1–6

    Google Scholar 

  13. Kim SM (2017) Microarray technology for identification of human papilloma virus subtype in oropharyngeal squamous cell carcinoma. Eur Arch Otorhinolaryngol 274:4255–4257

    Article  PubMed  Google Scholar 

  14. Mork J, Lie AK, Glattre E et al (2001) Human papillomavirus infection as a risk factor for squamous-cell carcinoma of the head and neck. N Engl J Med 345:376 (author reply 377)

    Article  Google Scholar 

  15. Herrero R, Castellsagué X, Pawlita M et al (2003) Human papillomavirus and oral cancer: the international agency for research on cancer multicenter study. J Natl Cancer Inst 95:1772–1783

    Article  PubMed  Google Scholar 

  16. Pintos J, Black MJ, Sadeghi N (2008) Human papillomavirus infection and oral cancer: a case-control study in Montreal, Canada. Oral Oncol 44:242–250

    Article  PubMed  Google Scholar 

  17. Huber MA, Tantiwongkosi B (2014) Oral and oropharyngeal cancer. Med Clin North Am 98:1299–1321

    Article  PubMed  Google Scholar 

  18. Saraiya M, Unger ER, Thompson TD et al (2015) US assessment of HPV types in cancers: implications for current and 9-valent HPV vaccines. J Natl Cancer Inst 107:1–12

  19. Albano PM, Holzinger D, Salvador C (2017) Low prevalence of human papillomavirus in head and neck squamous cell carcinoma in the northwest region of the Philippines. PLoS One 12:e0172240

    Article  PubMed  PubMed Central  Google Scholar 

  20. Lopes V, Murray P, Williams H et al (2011) Squamous cell carcinoma of the oral cavity rarely harbours oncogenic human papillomavirus. Oral Oncol 47:698–701

    Article  CAS  PubMed  Google Scholar 

  21. Marur S, D’Souza G, Westra WH, Forastiere AA (2010) HPV-associated head and neck cancer: a virus-related cancer epidemic. Lancet Oncol 11:781–789

    Article  PubMed  PubMed Central  Google Scholar 

  22. Rettig E, Kiess AP, Fakhey C (2015) The role of sexual behavior in head and neck cancer: implications for prevention and therapy. Expert Rev Anticancer Ther 15:35–49

    Article  CAS  PubMed  Google Scholar 

  23. Kreimer AR, Bhatia RK, Messeguer AL et al (2010) Oral human papillomavirus in healthy individuals: a systematic review of the literature. Sex Transm Dis 37:386–391

    PubMed  Google Scholar 

  24. The World Bank (2017) World development indicators. http://data.worldbank.org/data-catalog/GDP-ranking-table. Accessed 22 May 2017

  25. Ang K, Harris J, Wheeler R et al (2010) Human papillomavirus and survival of patients with oropharyngeal cancer. N Engl J Med 363:24–35

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Squier C (1991) The permeability of oral mucosa. Crit Rev Oral Biol Med 2:13–32

    Article  CAS  PubMed  Google Scholar 

  27. Saulle R, Semyonov L, Mannocci A (2015) Human papillomavirus and cancerous diseases of the head and neck: a systematic review and meta-analysis. Oral Dis 21:417–431

    Article  CAS  PubMed  Google Scholar 

  28. Combes JD, Franceschi S (2014) Role of human papillomavirus in non-oropharyngeal head and neck cancers. Oral Oncol 50:370–379

    Article  CAS  PubMed  Google Scholar 

  29. Rietbergen MM, Leemans CR, Bloemena E et al (2013) Increasing prevalence rates of HPV attributable oropharyngeal squamous cell carcinomas in the Netherlands as assessed by a validated test algorithm. Int J Cancer 132:1565–1571

    Article  CAS  PubMed  Google Scholar 

  30. Smeets SJ, Hesselink AT, Speel EJ et al (2007) A novel algorithm for reliable detection of human papillomavirus in paraffin embedded head and neck cancer specimen. Int J Cancer 121:2465–2472

    Article  CAS  PubMed  Google Scholar 

  31. Dayyani F, Etzel CJ, Liu M et al (2010) Meta-analysis of the impact of human papillomavirus (HPV) on cancer risk and overall survival in head and neck squamous cell carcinomas (HNSCC). Head Neck Oncol 2:15

    Article  PubMed  PubMed Central  Google Scholar 

  32. Ragin CCR, Taioli E (2007) Survival of squamous cell carcinoma of the head and neck in relation to human papillomavirus infection: review and meta-analysis. Int J Cancer 121:1813–1820

    Article  CAS  PubMed  Google Scholar 

  33. Ferlay J, Shin HR, Bray F et al (2010) Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 127:2893–2917

    Article  CAS  PubMed  Google Scholar 

  34. Gillison ML, Chaturvedi AK, Lowy DR (2008) HPV prophylactic vaccines and the potential prevention of noncervical cancers in both men and women. Cancer 113:3036–3046

    Article  PubMed  Google Scholar 

  35. Chaturvedi AK (2010) Beyond cervical cancer: burden of other HPV-related cancers among men and women. J Adolesc Health 46:S20–S26

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

This study was supported by SNUDH Research Fund (03-2014-0033) and the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2015K1A3A9A01028230 and 2017R1D1A1B04029339).

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Author notes

  1. Soung Min Kim and Ik Jae Kwon equal co-first authors.

Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul, 110-768, South Korea

    Soung Min Kim, Ik Jae Kwon, Hoon Myoung & Jong Ho Lee

  2. Department of Oral Pathology, College of Dentistry, Gangneung-Wonju National University, Gangneung, South Korea

    Suk Keun Lee

Authors
  1. Soung Min Kim
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  2. Ik Jae Kwon
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Corresponding authors

Correspondence to Hoon Myoung or Jong Ho Lee.

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Funding

This study had no funding.

Conflict of interest

There are no conflicts of interest in this article.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Institutional Review Board (IRBS-D2017006) at Seoul National University and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Kim, S.M., Kwon, I.J., Myoung, H. et al. Identification of human papillomavirus (HPV) subtype in oral cancer patients through microarray technology. Eur Arch Otorhinolaryngol 275, 535–543 (2018). https://doi.org/10.1007/s00405-017-4828-9

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  • DOI: https://doi.org/10.1007/s00405-017-4828-9

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