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Somatic embryogenesis and plant regeneration from different wild diploid cotton (Gossypium) species

  • Cell Biology and Morphogenesis
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Abstract

Calli were successfully induced from hypocotyls of eight wild diploid cotton species (Gossypium) on MSB (MS salts and B5 vitamins) medium supplemented with 0.09 μM 2,4-D (2,4-dichlorophenoxyacetic acid) and 2.32 μM KT (kinetin). Plant growth regulator (PGR) combinations, adding GA3 (Gibberellic acid), high inorganic salt stress, and PGR-free media were used to induce embryogenic calli from nonembryogenic calli. Embryogenic cultures were induced from G. aridum S. (D4 genome), G. davidsonii K. (D3-d genome), G. klotzschianum A. (D3-k genome), G. raimondii U. (D5 genome), and G. stocksii M. (E1 genome). We then observed somatic embryogenesis in the five species while calli of G. africanum V. (A1-2 genome), G. anomalum W. (B1 genome), and G. bickii P. (G genome) remained nonembryogenic. Somatic embryogenesis was adjusted by changing sugar sources, regulating combinations of PGRs, and using cell suspension culture. Embryos at various developmental stages produced mature and germinating embryos when cultured on filter paper placed on the media containing different sugar sources. The utility of different sugar sources promoted globular embryos developing into cotyledonary stage and increased the frequency of cotyledonary embryos developing into normal plants. Normal plantlets were regenerated from G. davidsonii, G. klotzschianum, G. raimondii, and G. stocksii. Only abnormal plantlets were obtained in G. aridum. This work will contribute to broadening the number of regenerable cotton species and provide foundations for somatic hybridization in cotton to create new germplasm.

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References

  • Chia JT, Saunders JW (1999) Evaluation of sole nitrogen sources for shoot and leaf disc cultures of sugarbeet. Plant Cell Tissue Organ Cult 59:47–56

    Article  Google Scholar 

  • Davidonis GH, Hamilton RH (1983) Plant regeneration from callus tissue of Gossypium hirsutum L. Plant Sci Lett 32:89–93

    Article  CAS  Google Scholar 

  • Finer JJ (1988) Plant regeneration from somatic embryogenic suspension cultures of cotton, Gossypium hirsutum L. Plant Cell Rep 8:586–589

    Article  Google Scholar 

  • Finer JJ, Smith RH (1984) Initiation of callus and somatic embryos from explants of mature cotton (Gossypium klotzschianum Anderss). Plant Cell Rep 3:41–43

    Article  Google Scholar 

  • Firoozabady E, DeBoer DL (1993) Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.). In Vitro Cell Dev Biol Plant 29:166–173

    Article  Google Scholar 

  • Firoozabady E, DeBoer DL, Merlo D, Halk E, Amerson L, Raska K, Murry E (1987) Transformation of cotton by Agrobacterium tumefaciens and regeneration of transgenic plants. Plant Mol Biol 19:105–116

    Article  Google Scholar 

  • Fryxell PA (1992) A revised taxonomic interpretation of Gossypium L. (Malvaceae). Rhoedea 2:108–165

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  • Kumria R, Sunnichan VG, Das DK, Gupta SK, Reddy VS, Bhatnagar RK, Leelavathi S (2003) High-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress. Plant Cell Rep 21:635–639

    PubMed  CAS  Google Scholar 

  • Mishra R, Wang HY, Yadav NR, Wilkins TA (2003) Development of highly regenerable elite Acala cotton (Gossypium hirsutum cv. Maxxa)—a step towards genotype-independent regeneration. Plant Cell Tissue Organ Cult 73:21–35

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) Revised media for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nobre J, Keith DJ, Dunwell JM (2001) Morphogenesis and regeneration from stomatal guard cell complexes of cotton (Gossypium hirsutum L.). Plant Cell Rep 20:8–15

    Article  CAS  Google Scholar 

  • Price HJ, Smith RH (1979) Somatic embryogenesis in suspension cultures of Gossypium klotzschianum Anderss. Planta 145:305–307

    Article  CAS  Google Scholar 

  • Rajasekaran K (1996) Regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (Gossypium hirsutum L.). Plant Cell Rep 15:859–864

    Article  CAS  Google Scholar 

  • Rajasekaran K, Sakhanokho HF, Zipf A, Saha S, Sharma GC, Peng WC (2004) Somatic embryo initiation and germination in diploid cotton (Gossypium arboreum L.). In Vitro Cell Dev Biol Plant 40:177–181

    Article  Google Scholar 

  • Sakhanokho HF, Zipt A, Rajasekaran K, Saha S, Sharma GC (2001) Induction of highly embryogenic calli and plant regeneration in upland (Gossypium hirsutum L.) and Pima (Gossypium barbadense L.) cottons. Crop Sci 41:1235–1240

    Article  Google Scholar 

  • Shoemaker RC, Couche LJ, Galbraith DW (1986) Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Rep 3:178–181

    Article  Google Scholar 

  • Smith RH, Price HJ, Thaxton JR (1977) Defined conditions for the initiation and growth of cotton callus in vitro. I. Gossypium arboreum. In Vitro 13:329–334

    Article  PubMed  CAS  Google Scholar 

  • Sun YQ, Zhang XL, Jin SX, Liang SG, Nie YC (2003) Somatic embryogenesis and plant regeneration in wild cotton (Gossypium klotzschianum Anderss). Plant Cell Tissue Organ Cult 75:247–253

    Article  CAS  Google Scholar 

  • Sun YQ, Zhang XL, Jin SX, Liang SG, Nie YC, Guo XP (2004) Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion. Theor Appl Genet 109:472–479

    Article  PubMed  CAS  Google Scholar 

  • Trolinder NL, Goodin JR (1987) Somatic embryogenesis and plant regeneration in Gossypium hirsutum L. Plant Cell Rep 6:231–234

    Article  CAS  Google Scholar 

  • Trolinder NL, Goodin JR (1988a) Somatic embryogenesis in cotton (Gossypium). I. Effects of source of explant and hormone regime. Plant Cell Tissue Organ Cult 12:31–42

    Article  CAS  Google Scholar 

  • Trolinder NL, Goodin JR (1988b) Somatic embryogenesis in cotton (Gossypium). II. Requirements for embryo development and plant regeneration. Plant Cell Tissue Organ Cult 12:43–53

    Article  CAS  Google Scholar 

  • Trolinder NL, Xhixian C (1989) Genotype specificity of the somatic embryogenesis response in cotton. Plant Cell Rep 8:133–136

    Article  Google Scholar 

  • Wilkins TA, Rajasekaran K, Anderson D (2000) Cotton biotechnology. Crit Rev Plant Sci 15:511–550

    Article  Google Scholar 

  • Wilkins TA, Mishra R, Trolinder NL (2004) Agrobacterium-mediated transformation and regeneration of cotton. J Food Agric Environ 2:179–187

    Google Scholar 

  • Wu JH, Zhang XL, Nie YC, Jin SX, Liang SG (2004) Factors affecting somatic embryogenesis and plant regeneration from a range of recalcitrant genotypes of Chinese cottons (Gossypium hirsutum L.). In Vitro Cell Dev Biol Plant 40:371–375

    Article  CAS  Google Scholar 

  • Zhang XL, Sun JZ, Liu JL (1991) Somatic embryogenesis and plant regeneration in upland cotton. Chin J Genet 18(5):461–467

    Google Scholar 

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Acknowledgements

We thank Dr. K. B. Wang, Cotton Research Institute of CAAS, for providing the germplasm used in this study, and Professor J McD Stewart (University of Arkansas, USA) for his fruitful comments. This study was supported by funds provided by the National Basic Research Program of China (2004CB117301).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, P.R. China

    Yuqiang Sun, Xianlong Zhang, Chao Huang, Xiaoping Guo & Yichun Nie

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  1. Yuqiang Sun
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  2. Xianlong Zhang
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  3. Chao Huang
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  4. Xiaoping Guo
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  5. Yichun Nie
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Correspondence to Xianlong Zhang.

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Communicated by Y. Lu

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Sun, Y., Zhang, X., Huang, C. et al. Somatic embryogenesis and plant regeneration from different wild diploid cotton (Gossypium) species. Plant Cell Rep 25, 289–296 (2006). https://doi.org/10.1007/s00299-005-0085-2

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  • DOI: https://doi.org/10.1007/s00299-005-0085-2

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