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Regulation of conidiation in Botrytis cinerea involves the light-responsive transcriptional regulators BcLTF3 and BcREG1

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Abstract

Botrytis cinerea is a plant pathogenic fungus with a broad host range. Due to its rapid growth and reproduction by asexual spores (conidia), which increases the inoculum pressure, the fungus is a serious problem in different fields of agriculture. The formation of the conidia is promoted by light, whereas the formation of sclerotia as survival structures occurs in its absence. Based on this observation, putative transcription factors (TFs) whose expression is induced upon light exposure have been considered as candidates for activating conidiation and/or repressing sclerotial development. Previous studies reported on the identification of six light-responsive TFs (LTFs), and two of them have been confirmed as crucial developmental regulators: BcLTF2 is the positive regulator of conidiation, whose expression is negatively regulated by BcLTF1. Here, the functional characterization of the four remaining LTFs is reported. BcLTF3 has a dual function, as it represses conidiophore development by repressing bcltf2 in light and darkness, and is moreover essential for conidiogenesis. In bcltf3 deletion mutants conidium initials grow out to hyphae, which develop secondary conidiophores. In contrast, no obvious functions could be assigned to BcLTF4, BcLTF5 and BcLTF6 in these experiments. BcREG1, previously reported to be required for virulence and conidiogenesis, has been re-identified as light-responsive transcriptional regulator. Studies with bcreg1 overexpression strains indicated that BcREG1 differentially affects conidiation by acting as a repressor of BcLTF2-induced conidiation in the light and as an activator of a BcLTF2-independent conidiation program in the dark.

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Acknowledgements

We thank Paul Tudzynski for support and discussion, Kim Cohrs for providing the expression data on the bctlf2 mutants and for help with microscopy, Bettina Richter and Charlotte Kaiser for their contributions to mutant generation, and Dominik Wagner for help with the microarray experiments. This study was supported by the Deutsche Forschungsgemeinschaft (SCHU 2833/4-1).

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  1. Institut für Biologie und Biotechnologie der Pflanzen (IBBP), Westfälische Wilhelms-Universität (WWU) Münster, Schlossplatz 8, 48143, Münster, Germany

    Beate Brandhoff, Anne Dornieden & Julia Schumacher

  2. UMR BIOGER, INRA, AgroParisTech, Université Paris-Saclay, 78850, Thiverval-Grignon, France

    Adeline Simon

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  1. Beate Brandhoff
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Brandhoff, B., Simon, A., Dornieden, A. et al. Regulation of conidiation in Botrytis cinerea involves the light-responsive transcriptional regulators BcLTF3 and BcREG1. Curr Genet 63, 931–949 (2017). https://doi.org/10.1007/s00294-017-0692-9

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