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Bacillus dafuensis sp. Nov., Isolated from a Forest Soil in China

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Abstract

A Gram-staining-positive, motile, rod-shaped, aerobic bacterial strain FJAT-25496 T was isolated from a forest soil sample collected from Dafu County, Chengdu City, Sichuan Province, China. Strain FJAT-25496 T grew at 15–50 °C (optimum 35 °C), pH 6.0–10.0 (optimum 8.0), and NaCl tolerance of 0–4.0% (w/v) (optimum 0%). The strain contained iso-C15:0 and anteiso-C15:0 as the predominant cellular fatty acids as well as MK-7 as the only menaquinone. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), and phosphatidyl glycerol (PG). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FJAT-25496 T was a member of the genus Bacillus, and most closely related to Bacillus solani FJAT-18043 T (97.8%), Bacillus praedii FJAT-25547 T (97.8%), Bacillus depressus BZ1T (97.7%), Bacillus oceanisediminis H2T (97.7%), and Bacillus ciccensis 5L6T (97.6%). Based on complete genome comparisons between strain FJAT-25496 T and the type strains of its closely related species, the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values were 73.4–76.3% and 21.7–23.2%, respectively. The genomic DNA G + C content of strain FJAT-25496 T was 36.9%. On the basis of phenotypic, phylogenetic, chemotaxonomic, and genomic features, strain FJAT-25496 T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus dafuensis sp. nov. is proposed. The type strain is FJAT-25496 T (= CCTCC AB 2019182 T = KCTC 43120 T).

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Abbreviations

ANI:

Average Nucleotide Identity

DPG:

Diphosphatidyl glycerol

PME:

Phosphatidyl methyl ethanolamine

PG:

Phosphatidyl glycerol

PC:

Phosphatidyl choline

UAPL:

Unidentified aminophospholipids

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31701835), the National Key R & D Program of China (2017YFD0201100 and 2016YFD0800605), Science and Technology Innovation Team Program (STIT 2017–1-11), and the Agrobiogenome Program (A2015-3,A2017-4) of Fujian Academy of Agricultural Sciences.

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Authors and Affiliations

  1. Agrobiological Resource Research Institute, Fujian Academy of Agriculture Sciences, Fuzhou, 350003, China

    Xuefang Zheng, Jieping Wang, Haifeng Zhang & Bo Liu

  2. Dean of Department of Biochemistry and Biotechnology, School of Life Sciences, Xiamen University, Xiamen, 361102, China

    Ziran Wang

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  1. Xuefang Zheng
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Correspondence to Bo Liu.

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Zheng, X., Liu, G., Wang, Z. et al. Bacillus dafuensis sp. Nov., Isolated from a Forest Soil in China. Curr Microbiol 77, 2049–2055 (2020). https://doi.org/10.1007/s00284-020-02014-2

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