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Performance and mechanism of triclosan removal in simultaneous nitrification and denitrification (SND) process under low-oxygen condition

  • Environmental biotechnology
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Abstract

Wastewater treatment under low dissolved oxygen (DO) conditions is promising for its low energy consumption. However, the removal process of some organic micropollutants, such as triclosan (TCS), could be inhibited under anaerobic conditions. So, it is worth investigating the TCS removal performance at low-oxygen condition. In this study, simultaneous nitrification and denitrification (SND) process, with DO ranging from 0.30 to 0.80 mg L−1, was chosen to investigate. Results showed that the water quality of the effluent was deteriorated after TCS addition at the beginning, with removal efficiency of NH4 +-N dropped from almost 100 ± 0.70 to 88.30 ± 0.98% and COD decreased from 95.15 ± 1.55 to 65.81 ± 2.42 %. However, the performance recovered from the 3rd day and almost stabilized on the 14th day with the removal efficiencies of NH4 +-N were over 98.00 ± 0.60 %, and COD was above 94.00 ± 1.70 % in effluent. Besides, TCS removal efficiencies were more than 93.00 %, and the contributions for TCS removal by the water effluent, sludge sorption, and other effects including biodegradation were 6.46 ± 2.25, 16.27 ± 3.30, and 77.27 ± 4.45 %, respectively. Although the results of absolute abundances of related genes showed no difference (P > 0.05), Illumina MiSeq sequencing analysis presented the variation of microbial community after TCS addition, in which T-45 had the highest Shannon and Simpson diversity index, followed by T-0 and T-2. Relative abundances of alpha and beta-Proteobacteria, which were related to TCS biodegradation, were increased. Compared with Bacteroidetes in T-0, the abundance of Bacteroidetes took up more than 15.6 % in T-45, which should play a more important role under low-oxygen conditions with TCS addition.

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References

  • Ansola G, Arroyo P, de Miera LES (2014) Characterisation of the soil bacterial community structure and composition of natural and constructed wetlands. Sci Total Environ 473:63–71

    Article  PubMed  Google Scholar 

  • Apha (2005) Standard methods for the examination of water and wastewater. American Public Health Association, American, Washington D.C.

  • Balmer ME, Poiger T, Droz C, Romanin K, Bergqvist P-A, Müller MD, Buser H-R (2004) Occurrence of methyl triclosan, a transformation product of the bactericide triclosan, in fish from various lakes in Switzerland. Environ Sci Technol 38:390–395

    Article  CAS  PubMed  Google Scholar 

  • Banihashemi B, Droste RL (2014) Sorption–desorption and biosorption of bisphenol a, triclosan, and 17α-ethinylestradiol to sewage sludge. Sci Total Environ 487:813–821

    Article  CAS  PubMed  Google Scholar 

  • Bauer M, Kube M, Teeling H, Richter M, Lombardot T, Allers E, Würdemann CA, Quast C, Kuhl H, Knaust F (2006) Whole genome analysis of the marine bacteroidetes ‘Gramella forsetii’reveals adaptations to degradation of polymeric organic matter. Environ Microbiol 8:2201–2213

    Article  CAS  PubMed  Google Scholar 

  • Bester K (2003) Triclosan in a sewage treatment process—balances and monitoring data. Water Res 37:3891–3896

    Article  CAS  PubMed  Google Scholar 

  • Bester K (2005) Fate of triclosan and triclosan-methyl in sewage treatment plants and surface waters. Arch Environ Con Tox 49:9–17

    Article  CAS  Google Scholar 

  • Bokare V, Murugesan K, Kim Y-M, Jeon J-R, Kim E-J, Chang YS (2010) Degradation of triclosan by an integrated nano-bio redox process. Bioresour Technol 101:6354–6360

    Article  CAS  PubMed  Google Scholar 

  • Chalew TE, Halden RU (2009) Environmental exposure of aquatic and terrestrial biota to triclosan and triclocarban1. JAWRA J Am Water Resour As 45:4–13

    Article  CAS  Google Scholar 

  • Chen X, Richard J, Liu Y, Dopp E, Tuerk J, Bester K (2012) Ozonation products of triclosan in advanced wastewater treatment. Water Res 46:2247–2256

    Article  CAS  PubMed  Google Scholar 

  • Curtis TP (2010) Low-energy wastewater treatment: strategies and technologies. Environ Microbiol, 2nd edn. Wiley, New York, pp 301–318

  • Drury B, Scott J, Rosi-Marshall EJ, Kelly JJ (2013) Triclosan exposure increases triclosan resistance and influences taxonomic composition of benthic bacterial communities. Environ Sci Technol 47:8923–8930

    CAS  PubMed  Google Scholar 

  • Fang J-L, Stingley RL, Beland FA, Harrouk W, Lumpkins DL, Howard P (2010) Occurrence, efficacy, metabolism, and toxicity of triclosan. J Environmenta Sci Heal, Part C 28:147–171

    Article  CAS  Google Scholar 

  • Federle TW, Kaiser SK, Nuck BA (2002) Fate and effects of triclosan in activated sludge. Environ Toxicol Chem 21:1330–1337

    Article  CAS  PubMed  Google Scholar 

  • Good IJ (1953) The population frequencies of species and the estimation of population parameters. Biometrika 40:237–264

    Article  Google Scholar 

  • Heidler J, Halden RU (2007) Mass balance assessment of triclosan removal during conventional sewage treatment. Chemosphere 66:362–369

    Article  CAS  PubMed  Google Scholar 

  • Hocaoglu SM, Insel G, Cokgor EU, Orhon D (2011) Effect of low dissolved oxygen on simultaneous nitrification and denitrification in a membrane bioreactor treating black water. Bioresour Technol 102:4333–4340

    Article  CAS  PubMed  Google Scholar 

  • Holman J, Wareham D (2005) COD, ammonia and dissolved oxygen time profiles in the simultaneous nitrification/denitrification process. Biochem Eng J 22:125–133

    Article  CAS  Google Scholar 

  • Huse SM, Dethlefsen L, Huber JA, Welch DM, Relman DA, Sogin ML (2008) Exploring microbial diversity and taxonomy using SSU rRNA hypervariable tag sequencing. PLoS Genet 4:e1000255

    Article  PubMed  PubMed Central  Google Scholar 

  • Jia W, Liang S, Zhang J, Ngo HH, Guo W, Yan Y, Zou Y (2013) Nitrous oxide emission in low-oxygen simultaneous nitrification and denitrification process: sources and mechanisms. Bioresour Technol 136:444–451

    Article  CAS  PubMed  Google Scholar 

  • Kookana R, Ying G, Waller N (2011) Triclosan: its occurrence, fate and effects in the Australian environment. Water Sci Technol 63:598–604

    Article  CAS  PubMed  Google Scholar 

  • Kwon JW, Xia K (2012) Fate of triclosan and triclocarban in soil columns with and without biosolids surface application. Environ Toxicol Chem 31:262–269

    Article  CAS  PubMed  Google Scholar 

  • Lee DG, Cho K-C, Chu K-H (2014) Identification of triclosan-degrading bacteria in a triclosan enrichment culture using stable isotope probing. Biodegradation 25:55–65

    Article  CAS  PubMed  Google Scholar 

  • Lee DG, Chu K-H (2015) Abundances of triclosan-degrading microorganisms in activated sludge systems. Environ Eng Res 20:105–109

    Article  Google Scholar 

  • Li H, Shen T-T, Wang X-L, Lin K-F, Liu Y-D, Lu S-G, Gu J-D, Wang P, Lu Q, Du X-M (2013) Biodegradation of perchloroethylene and chlorophenol co-contamination and toxic effect on activated sludge performance. Bioresour Technol 137:286–293

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Shi H, Xia L, Shi H, Shen T, Wang Z, Wang G, Wang Y (2010) Study of operational conditions of simultaneous nitrification and denitrification in a carrousel oxidation ditch for domestic wastewater treatment. Bioresour Technol 101:901–906

    Article  CAS  PubMed  Google Scholar 

  • Lozano N, Rice CP, Ramirez M, Torrents A (2013) Fate of triclocarban, triclosan and methyltriclosan during wastewater and biosolids treatment processes. Water Res 47:4519–4527

    Article  CAS  PubMed  Google Scholar 

  • McAvoy DC, Schatowitz B, Jacob M, Hauk A, Eckhoff WS (2002) Measurement of triclosan in wastewater treatment systems. Environ Toxicol Chem 21:1323–1329

    Article  CAS  PubMed  Google Scholar 

  • McMurry LM, Oethinger M, Levy SB (1998) Triclosan targets lipid synthesis. Nature 394:531–532

    Article  CAS  PubMed  Google Scholar 

  • McNamara PJ, LaPara TM, Novak PJ (2014) The impacts of triclosan on anaerobic community structures, function, and antimicrobial resistance. Environ Sci Technol 48:7393–7400

    Article  CAS  PubMed  Google Scholar 

  • Meade MJ, Waddell RL, Callahan TM (2001) Soil bacteria Pseudomonas putida and Alcaligenes xylosoxidans subsp. denitrificans inactivate triclosan in liquid and solid substrates. FEMS Microbiol Lett 204:45–48

    Article  CAS  PubMed  Google Scholar 

  • Podosokorskaya OA, Kadnikov VV, Gavrilov SN, Mardanov AV, Merkel AY, Karnachuk OV, Ravin NV, Bonch-Osmolovskaya EA, Kublanov IV (2013) Characterization of Melioribacter roseus gen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria, and a proposal of a novel bacterial phylum Ignavibacteriae. Environ Microbiol 15:1759–1771

    Article  CAS  PubMed  Google Scholar 

  • Roh H, Subramanya N, Zhao F, Yu C-P, Sandt J, Chu K-H (2009) Biodegradation potential of wastewater micropollutants by ammonia-oxidizing bacteria. Chemosphere 77:1084–1089

    Article  CAS  PubMed  Google Scholar 

  • Russell A (2004) Whither triclosan? J Antimicrob Chemother 53:693–695

    Article  CAS  PubMed  Google Scholar 

  • Singer H, Müller S, Tixier C, Pillonel L (2002) Triclosan: occurrence and fate of a widely used biocide in the aquatic environment: field measurements in wastewater treatment plants, surface waters, and lake sediments. Environ Sci Technol 36:4998–5004

    Article  CAS  PubMed  Google Scholar 

  • Stasinakis AS, Mamais D, Thomaidis NS, Danika E, Gatidou G, Lekkas TD (2008) Inhibitory effect of triclosan and nonylphenol on respiration rates and ammonia removal in activated sludge systems. Ecotoxicol Environ Saf 70:199–206

    Article  CAS  PubMed  Google Scholar 

  • Stasinakis AS, Petalas AV, Mamais D, Thomaidis NS, Gatidou G, Lekkas TD (2007) Investigation of triclosan fate and toxicity in continuous-flow activated sludge systems. Chemosphere 68:375–381

    Article  CAS  PubMed  Google Scholar 

  • Thompson A, Griffin P, Stuetz R, Cartmell E (2005) The fate and removal of triclosan during wastewater treatment. Water Environ Res 77:63–67

    Article  CAS  PubMed  Google Scholar 

  • Waltman EL, Venables BJ, Waller WT (2006) Triclosan in a North Texas wastewater treatment plant and the influent and effluent of an experimental constructed wetland. Environ Toxicol Chem 25:367–372

    Article  CAS  PubMed  Google Scholar 

  • Ying G-G, Kookana RS (2007) Triclosan in wastewaters and biosolids from Australian wastewater treatment plants. Environ Int 33:199–205

    Article  CAS  PubMed  Google Scholar 

  • Ying G-G, Yu X-Y, Kookana RS (2007) Biological degradation of triclocarban and triclosan in a soil under aerobic and anaerobic conditions and comparison with environmental fate modelling. Environ Pollut 150:300–305

    Article  CAS  PubMed  Google Scholar 

  • Zeng RJ, Lemaire R, Yuan Z, Keller J (2003) Simultaneous nitrification, denitrification, and phosphorus removal in a lab-scale sequencing batch reactor. Biotechnol Bioeng 84:170–178

    Article  CAS  PubMed  Google Scholar 

  • Zhao C, Xie H, Xu J, Xu X, Zhang J, Hu Z, Liu C, Liang S, Wang Q, Wang J (2015) Bacterial community variation and microbial mechanism of triclosan (TCS) removal by constructed wetlands with different types of plants. Sci Total Environ 505:633–639

    Article  CAS  PubMed  Google Scholar 

  • Zhou Y, Jiang X (2012) Dissecting android malware: characterization and evolution. 2012 I.E. Symposium on Security and Privacy. doi: 10.1109/SP.2012.16

Download references

Acknowledgements

This research was funded by Shandong Provincial Natural Science (ZR2015BM004), the Fundamental Research Funds of Shandong University (2014JC023), the National Science Foundation of China (21007032), and the National Water Special Project (No. 2012ZX07203-004).

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Authors and Affiliations

  1. Environment Research Institute, Shandong University, Jinan, 250100, China

    Junhua Liu, Jingmin Wang, Jianing Liu & Huijun Xie

  2. School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China

    Congcong Zhao, Jian Zhang & Zhen Hu

  3. State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China

    Shuning Wang

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  1. Junhua Liu
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  2. Jingmin Wang
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Correspondence to Huijun Xie.

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Liu, J., Wang, J., Zhao, C. et al. Performance and mechanism of triclosan removal in simultaneous nitrification and denitrification (SND) process under low-oxygen condition. Appl Microbiol Biotechnol 101, 1653–1660 (2017). https://doi.org/10.1007/s00253-016-7952-3

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