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Enhancement of polysaccharides production in Ganoderma lucidum by the addition of ethyl acetate extracts from Eupolyphaga sinensis and Catharsius molossus

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Abstract

To screen stimulators from Chinese medicinal insects for mycelial growth and polysaccharides production of Ganoderma lucidum, G. lucidum was inoculated into the media with and without supplementation of medicinal insect extracts. The ethyl acetate extract of Eupolyphaga sinensis at 55 mg l−1 lead to significant increase in both biomass and intracellular polysaccharides (IPS) concentration from 8.53 ± 0.41 to 14.16 ± 0.43 and 1.28 ± 0.09 to 2.13 ± 0.11 g l−1, respectively. In addition, the ethyl acetate extract of Catharsius molossus at 55 mg l−1 significantly enhanced extracellular polysaccharides (EPS) production; the EPS yield increased from 350.9 ± 14.1 to 475.1 ± 15.3 mg l−1. There were no new components in the two types of polysaccharides obtained by the addition of the insect extracts.

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Acknowledgement

The authors would like to thank the financial support by The 10th Five Years Key Programs for Science and Technology Development of China (200113A501A01). G.-Q. Liu also thanks Mr. Jian Wu for his helpful assistance in cell culture.

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, 170 Huihe Road, Wuxi, 214036, People’s Republic of China

    Gao-Qiang Liu & Ke-Chang Zhang

  2. College of Life Sciences and Technology, Central South University of Forestry and Technology, 498 South Shaoshan Road, Changsha, 410004, People’s Republic of China

    Gao-Qiang Liu

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  1. Gao-Qiang Liu
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  2. Ke-Chang Zhang
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Correspondence to Ke-Chang Zhang.

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Liu, GQ., Zhang, KC. Enhancement of polysaccharides production in Ganoderma lucidum by the addition of ethyl acetate extracts from Eupolyphaga sinensis and Catharsius molossus . Appl Microbiol Biotechnol 74, 572–577 (2007). https://doi.org/10.1007/s00253-006-0709-7

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  • DOI: https://doi.org/10.1007/s00253-006-0709-7

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