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Shallow Water Marine Sediment Bacterial Community Shifts Along a Natural CO2 Gradient in the Mediterranean Sea Off Vulcano, Italy

  • Environmental Microbiology
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Abstract

The effects of increasing atmospheric CO2 on ocean ecosystems are a major environmental concern, as rapid shoaling of the carbonate saturation horizon is exposing vast areas of marine sediments to corrosive waters worldwide. Natural CO2 gradients off Vulcano, Italy, have revealed profound ecosystem changes along rocky shore habitats as carbonate saturation levels decrease, but no investigations have yet been made of the sedimentary habitat. Here, we sampled the upper 2 cm of volcanic sand in three zones, ambient (median pCO2 419 μatm, minimum Ωarag 3.77), moderately CO2-enriched (median pCO2 592 μatm, minimum Ωarag 2.96), and highly CO2-enriched (median pCO2 1611 μatm, minimum Ωarag 0.35). We tested the hypothesis that increasing levels of seawater pCO2 would cause significant shifts in sediment bacterial community composition, as shown recently in epilithic biofilms at the study site. In this study, 454 pyrosequencing of the V1 to V3 region of the 16S rRNA gene revealed a shift in community composition with increasing pCO2. The relative abundances of most of the dominant genera were unaffected by the pCO2 gradient, although there were significant differences for some 5 % of the genera present (viz. Georgenia, Lutibacter, Photobacterium, Acinetobacter, and Paenibacillus), and Shannon Diversity was greatest in sediments subject to long-term acidification (>100 years). Overall, this supports the view that globally increased ocean pCO2 will be associated with changes in sediment bacterial community composition but that most of these organisms are resilient. However, further work is required to assess whether these results apply to other types of coastal sediments and whether the changes in relative abundance of bacterial taxa that we observed can significantly alter the biogeochemical functions of marine sediments.

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Acknowledgments

This work was partly supported by a grant from the National Research Foundation (NRF) grant funded by the Korean government, Ministry of Education, Science and Technology (MEST) (NRF-2013-031400). This work was also partly supported by the Global Frontier Project, Centre of Integrated Smart Sensors funded by Ministry of Education Science and Technology, Korea (2012M3A6A6054201). DK is supported by the Korean Government Scholarship Program, Ministry of Education, Science, and Technology, South Korea. This work contributes to the EU FP7 project “Mediterranean Sea Acidification under a changing climate” (grant agreement no. 265103), with additional funding from Save Our Seas Foundation.

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Authors and Affiliations

  1. Department of Biological Sciences, Seoul National University, Gwanak-Gu, Seoul, 151-747, Republic of Korea

    Dorsaf Kerfahi, Binu M. Tripathi & Jonathan M. Adams

  2. Marine Biology and Ecology Research Centre, Plymouth University, Plymouth, PL4 8AA, UK

    Jason M. Hall-Spencer

  3. Dipartimento di Scienze della Terra e del Mare, University of Palermo, via Archirafi 28, 90123, Palermo, Italy

    Marco Milazzo

  4. School of Chemical and Biological Engineering, Seoul National University, Gwanak-Gu, Seoul, 151-747, Republic of Korea

    Dorsaf Kerfahi

  5. School of Mechanical and Aerospace Engineering, Seoul National University, Gwanak-Gu, Seoul, 151-747, Republic of Korea

    Junghoon Lee

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  1. Dorsaf Kerfahi
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  4. Marco Milazzo
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Correspondence to Junghoon Lee or Jonathan M. Adams.

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Kerfahi, D., Hall-Spencer, J.M., Tripathi, B.M. et al. Shallow Water Marine Sediment Bacterial Community Shifts Along a Natural CO2 Gradient in the Mediterranean Sea Off Vulcano, Italy. Microb Ecol 67, 819–828 (2014). https://doi.org/10.1007/s00248-014-0368-7

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